Nitrosopumilus maritimus (strain SCM1)

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrosopumilales; Nitrosopumilaceae; Nitrosopumilus; Nitrosopumilus maritimus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9A5B0|A9A5B0_NITMS Pyruvate flavodoxin/ferredoxin oxidoreductase domain protein OS=Nitrosopumilus maritimus (strain SCM1) OX=436308 GN=Nmar_0413 PE=4 SV=1
MM1 pKa = 7.48NKK3 pKa = 10.02YY4 pKa = 10.02LIPIIAIAIVGSVSAFAISPYY25 pKa = 8.94FTEE28 pKa = 4.2SSIDD32 pKa = 3.44EE33 pKa = 4.52ALPTGAVVQPMMEE46 pKa = 4.06EE47 pKa = 3.72TMMEE51 pKa = 3.85KK52 pKa = 7.98MTPVSYY58 pKa = 10.58SGTFIGVGDD67 pKa = 5.46GIHH70 pKa = 7.25DD71 pKa = 4.22AQGDD75 pKa = 4.28AYY77 pKa = 9.51TIPLEE82 pKa = 4.79DD83 pKa = 4.2GSDD86 pKa = 3.64VLRR89 pKa = 11.84LEE91 pKa = 4.84NFQSTNGPDD100 pKa = 3.32LYY102 pKa = 11.37VYY104 pKa = 10.83LATDD108 pKa = 3.77DD109 pKa = 4.51DD110 pKa = 4.65ASEE113 pKa = 4.66FINLGEE119 pKa = 4.1LKK121 pKa = 10.67ANKK124 pKa = 9.73GNQNYY129 pKa = 7.91EE130 pKa = 3.91IPDD133 pKa = 4.05DD134 pKa = 4.91ADD136 pKa = 3.28LTKK139 pKa = 10.88YY140 pKa = 10.8NKK142 pKa = 9.72VLIWCQAFSVLFGSAEE158 pKa = 3.91LSS160 pKa = 3.49

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A9A5J1|RS19_NITMS 30S ribosomal protein S19 OS=Nitrosopumilus maritimus (strain SCM1) OX=436308 GN=rps19 PE=3 SV=1
MM1 pKa = 7.42AMSRR5 pKa = 11.84AKK7 pKa = 10.31RR8 pKa = 11.84AEE10 pKa = 3.93AAKK13 pKa = 10.22KK14 pKa = 9.66AARR17 pKa = 11.84TRR19 pKa = 11.84KK20 pKa = 9.68RR21 pKa = 11.84NAAKK25 pKa = 10.3KK26 pKa = 9.69AAEE29 pKa = 4.03AAKK32 pKa = 10.2AAASRR37 pKa = 11.84KK38 pKa = 9.18RR39 pKa = 11.84KK40 pKa = 9.27AARR43 pKa = 11.84KK44 pKa = 9.38RR45 pKa = 11.84NAAKK49 pKa = 10.21KK50 pKa = 9.2AAPKK54 pKa = 10.32RR55 pKa = 11.84RR56 pKa = 11.84TAAKK60 pKa = 9.47KK61 pKa = 9.45AAPKK65 pKa = 10.27RR66 pKa = 11.84RR67 pKa = 11.84TAAKK71 pKa = 9.88RR72 pKa = 11.84KK73 pKa = 8.72AAPKK77 pKa = 10.04RR78 pKa = 11.84KK79 pKa = 7.37TAAKK83 pKa = 9.84RR84 pKa = 11.84KK85 pKa = 8.7AAPKK89 pKa = 10.02RR90 pKa = 11.84KK91 pKa = 8.69AAKK94 pKa = 9.87RR95 pKa = 11.84KK96 pKa = 8.76AAPKK100 pKa = 10.04RR101 pKa = 11.84KK102 pKa = 7.37TAAKK106 pKa = 9.84RR107 pKa = 11.84KK108 pKa = 8.7AAPKK112 pKa = 10.0RR113 pKa = 11.84KK114 pKa = 9.28AATKK118 pKa = 10.14RR119 pKa = 11.84RR120 pKa = 11.84AAPKK124 pKa = 9.76RR125 pKa = 11.84KK126 pKa = 8.49AAKK129 pKa = 9.71RR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 3.63

Molecular weight: 14.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1795

0

1795

493620

36

9585

275.0

30.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.079 ± 0.068

0.955 ± 0.027

6.147 ± 0.068

7.312 ± 0.072

4.404 ± 0.063

6.471 ± 0.076

1.772 ± 0.031

8.45 ± 0.077

8.352 ± 0.14

8.446 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.061

4.906 ± 0.048

3.902 ± 0.064

3.22 ± 0.044

3.409 ± 0.047

7.204 ± 0.081

5.75 ± 0.151

6.658 ± 0.058

0.892 ± 0.02

3.083 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski