Drosophila virilis (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4LSM7|B4LSM7_DROVI Uncharacterized protein isoform A OS=Drosophila virilis OX=7244 GN=Dvir\GJ16490 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84LISLVIFCLCVCAAIWPANALQCYY26 pKa = 8.15QCIGSEE32 pKa = 4.5CDD34 pKa = 4.64DD35 pKa = 4.82LDD37 pKa = 3.94DD38 pKa = 5.46SKK40 pKa = 11.8VVSCNLDD47 pKa = 3.39EE48 pKa = 4.46TTSTLTTEE56 pKa = 4.24DD57 pKa = 3.63TTTEE61 pKa = 4.18SLPDD65 pKa = 3.49TSTTSSGSSTGGSTTSPGPTVDD87 pKa = 3.59TPATEE92 pKa = 4.21SSPPSTTDD100 pKa = 3.08QSDD103 pKa = 3.63TTTEE107 pKa = 4.08ATSEE111 pKa = 4.19TTTTGEE117 pKa = 4.02PSEE120 pKa = 4.33STEE123 pKa = 4.12TTEE126 pKa = 4.24TTTGEE131 pKa = 4.13PSGSTEE137 pKa = 4.2TTDD140 pKa = 3.14STTEE144 pKa = 3.95EE145 pKa = 4.29PSGSTEE151 pKa = 4.06TTEE154 pKa = 4.12STTEE158 pKa = 3.87STTDD162 pKa = 3.19STSDD166 pKa = 3.41STSDD170 pKa = 3.44STSDD174 pKa = 3.49STSDD178 pKa = 3.37SSPGSTASSTSEE190 pKa = 3.94STSEE194 pKa = 4.09STSDD198 pKa = 3.43STSDD202 pKa = 3.48STSDD206 pKa = 3.27SSSGSTAGSTSDD218 pKa = 3.41STSEE222 pKa = 4.09STSDD226 pKa = 3.42STSDD230 pKa = 3.19STTGSTSDD238 pKa = 3.48STSDD242 pKa = 3.44STSDD246 pKa = 3.38STSSPEE252 pKa = 3.7SSIPSSSGSTEE263 pKa = 3.86SSTAASTGSSEE274 pKa = 4.4TGEE277 pKa = 4.39STTSGSTEE285 pKa = 3.98SSTSSSASVVNPGNLEE301 pKa = 4.26NIQLALQQAALPGGIRR317 pKa = 11.84SGRR320 pKa = 11.84ARR322 pKa = 11.84RR323 pKa = 11.84SLAAQAADD331 pKa = 3.3QLIRR335 pKa = 11.84NYY337 pKa = 10.52RR338 pKa = 11.84SYY340 pKa = 11.16EE341 pKa = 3.86FNGRR345 pKa = 11.84ATNYY349 pKa = 7.59RR350 pKa = 11.84TTACYY355 pKa = 10.34SIEE358 pKa = 4.12KK359 pKa = 10.25DD360 pKa = 3.43GEE362 pKa = 4.13IQRR365 pKa = 11.84GCVWIPEE372 pKa = 4.39GQSGCQAVRR381 pKa = 11.84EE382 pKa = 4.3KK383 pKa = 11.25VDD385 pKa = 3.57LQQNSVNDD393 pKa = 3.96LCDD396 pKa = 3.03VCQTDD401 pKa = 3.3NCNGSASLRR410 pKa = 11.84MSLAALLLLLGLGLRR425 pKa = 11.84SLFF428 pKa = 3.96

Molecular weight: 43.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4LZV7|B4LZV7_DROVI Uncharacterized protein OS=Drosophila virilis OX=7244 GN=Dvir\GJ23227 PE=4 SV=2
MM1 pKa = 6.69VQTGISLNEE10 pKa = 4.84RR11 pKa = 11.84FTQMQSRR18 pKa = 11.84QQQPPAAARR27 pKa = 11.84GRR29 pKa = 11.84RR30 pKa = 11.84SRR32 pKa = 11.84SRR34 pKa = 11.84SRR36 pKa = 11.84SRR38 pKa = 11.84RR39 pKa = 11.84IVDD42 pKa = 3.85AAPSVANSRR51 pKa = 11.84LLQEE55 pKa = 5.38FKK57 pKa = 10.53RR58 pKa = 11.84KK59 pKa = 8.51HH60 pKa = 4.68TVQTALKK67 pKa = 9.27LKK69 pKa = 10.03RR70 pKa = 11.84RR71 pKa = 11.84SLRR74 pKa = 11.84TTAGGASARR83 pKa = 11.84LGGIKK88 pKa = 10.39ALRR91 pKa = 11.84LGANGKK97 pKa = 7.97PVRR100 pKa = 11.84SNNGTRR106 pKa = 11.84LATMRR111 pKa = 11.84ADD113 pKa = 5.64LIANNNNGRR122 pKa = 11.84ARR124 pKa = 11.84RR125 pKa = 11.84SSSSARR131 pKa = 11.84RR132 pKa = 11.84SSAAAGGLGQRR143 pKa = 11.84LGLRR147 pKa = 11.84RR148 pKa = 11.84PVVGGAAEE156 pKa = 3.94RR157 pKa = 11.84VAQRR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84GLSRR167 pKa = 11.84QRR169 pKa = 11.84QQQQPLPQLQSRR181 pKa = 11.84GRR183 pKa = 11.84SRR185 pKa = 11.84SRR187 pKa = 11.84GRR189 pKa = 11.84ARR191 pKa = 11.84SLSRR195 pKa = 11.84GRR197 pKa = 11.84DD198 pKa = 3.32QSRR201 pKa = 11.84GRR203 pKa = 11.84SQSVGRR209 pKa = 11.84AQSRR213 pKa = 11.84GRR215 pKa = 11.84RR216 pKa = 11.84QLSANGGRR224 pKa = 11.84GVSAGGGGGRR234 pKa = 11.84APGRR238 pKa = 11.84VSVKK242 pKa = 9.43QRR244 pKa = 11.84LGVRR248 pKa = 11.84PGVAAAAAAAAKK260 pKa = 9.98GAANKK265 pKa = 9.95RR266 pKa = 11.84RR267 pKa = 11.84GNRR270 pKa = 11.84RR271 pKa = 11.84GNSQMRR277 pKa = 11.84GVTAGRR283 pKa = 11.84IDD285 pKa = 3.36KK286 pKa = 10.44RR287 pKa = 11.84RR288 pKa = 11.84NSSNTAQKK296 pKa = 8.76TVKK299 pKa = 10.07AAAGGRR305 pKa = 11.84GRR307 pKa = 11.84KK308 pKa = 8.93GRR310 pKa = 11.84ARR312 pKa = 11.84GRR314 pKa = 11.84SAVRR318 pKa = 11.84AAAAAVDD325 pKa = 3.7AASAATHH332 pKa = 6.76RR333 pKa = 11.84SRR335 pKa = 11.84SRR337 pKa = 11.84SRR339 pKa = 11.84SRR341 pKa = 11.84SRR343 pKa = 11.84ARR345 pKa = 11.84QGKK348 pKa = 8.32VAAGAAASSNGRR360 pKa = 11.84QKK362 pKa = 11.1GAGPVGARR370 pKa = 11.84RR371 pKa = 11.84RR372 pKa = 11.84GRR374 pKa = 11.84SLQRR378 pKa = 11.84TGVTVARR385 pKa = 11.84GANRR389 pKa = 11.84NGNKK393 pKa = 9.32KK394 pKa = 10.46KK395 pKa = 10.73NIITEE400 pKa = 4.35GVKK403 pKa = 10.27PNQLQQGRR411 pKa = 11.84RR412 pKa = 11.84GRR414 pKa = 11.84SRR416 pKa = 11.84GRR418 pKa = 11.84GGRR421 pKa = 11.84TVAGKK426 pKa = 7.53PQRR429 pKa = 11.84RR430 pKa = 11.84EE431 pKa = 3.91VKK433 pKa = 10.54RR434 pKa = 11.84EE435 pKa = 3.86DD436 pKa = 4.12LDD438 pKa = 4.45NEE440 pKa = 4.2LDD442 pKa = 3.56QYY444 pKa = 11.42MSTTKK449 pKa = 10.68SEE451 pKa = 3.92MDD453 pKa = 3.72FLLKK457 pKa = 10.68

Molecular weight: 48.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13562

4754

18316

11705031

17

17765

639.1

71.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.904 ± 0.024

1.891 ± 0.024

5.178 ± 0.016

6.384 ± 0.032

3.381 ± 0.013

5.802 ± 0.026

2.621 ± 0.011

4.941 ± 0.017

5.518 ± 0.028

9.121 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.011

4.893 ± 0.015

5.395 ± 0.024

5.55 ± 0.028

5.476 ± 0.014

8.093 ± 0.029

5.862 ± 0.016

5.713 ± 0.016

0.982 ± 0.006

2.998 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski