Pseudorhizobium banfieldiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Pseudorhizobium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0NCR7|L0NCR7_9RHIZ 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Pseudorhizobium banfieldiae OX=1125847 GN=fabF PE=3 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAQAADD24 pKa = 4.11AIIAAEE30 pKa = 4.42PEE32 pKa = 3.69PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.83TCLKK58 pKa = 9.33IGGYY62 pKa = 9.13VRR64 pKa = 11.84FQVDD68 pKa = 4.02FDD70 pKa = 4.27SRR72 pKa = 11.84DD73 pKa = 3.04GAYY76 pKa = 10.31ADD78 pKa = 5.15DD79 pKa = 5.51NWDD82 pKa = 2.43AWTRR86 pKa = 11.84GRR88 pKa = 11.84VDD90 pKa = 4.03FTAKK94 pKa = 10.33SDD96 pKa = 3.87TEE98 pKa = 4.27LGEE101 pKa = 3.97LTGFIAIQAEE111 pKa = 4.18ASDD114 pKa = 4.41YY115 pKa = 10.6PAATGSTGDD124 pKa = 3.48FYY126 pKa = 11.5FDD128 pKa = 3.34EE129 pKa = 5.54VYY131 pKa = 10.82LQLGGFKK138 pKa = 10.6AGTYY142 pKa = 9.78LNWWDD147 pKa = 3.62KK148 pKa = 11.15GINGEE153 pKa = 4.23TDD155 pKa = 3.07SLGATTRR162 pKa = 11.84MTSIAYY168 pKa = 9.31IYY170 pKa = 10.37TGDD173 pKa = 3.76AFTAGLQLDD182 pKa = 3.93EE183 pKa = 4.56LTNVEE188 pKa = 4.36LGGGGGQDD196 pKa = 3.5FGLEE200 pKa = 4.28AIVTASLGAASVDD213 pKa = 3.71LLGSYY218 pKa = 10.86DD219 pKa = 3.6FANEE223 pKa = 3.94DD224 pKa = 3.45GAVRR228 pKa = 11.84ALVSAGIGPGTLQLAGVWASGYY250 pKa = 9.52NVYY253 pKa = 11.02YY254 pKa = 10.43NASEE258 pKa = 3.9WTIAASYY265 pKa = 10.26SFKK268 pKa = 10.73ATDD271 pKa = 3.99KK272 pKa = 10.21FTITPGVQYY281 pKa = 10.06FSNVDD286 pKa = 3.52LDD288 pKa = 4.83GDD290 pKa = 4.74DD291 pKa = 5.38FDD293 pKa = 7.21GDD295 pKa = 4.15DD296 pKa = 3.36AWRR299 pKa = 11.84AGLTVDD305 pKa = 3.69YY306 pKa = 10.64AITSGLAMKK315 pKa = 10.56VSAQWNDD322 pKa = 2.76GNRR325 pKa = 11.84RR326 pKa = 11.84GAGDD330 pKa = 3.63EE331 pKa = 4.54DD332 pKa = 4.28YY333 pKa = 10.92WDD335 pKa = 3.61GFVRR339 pKa = 11.84LQRR342 pKa = 11.84SFF344 pKa = 3.42

Molecular weight: 36.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0NLI9|L0NLI9_9RHIZ N-acetyltransferase domain-containing protein OS=Pseudorhizobium banfieldiae OX=1125847 GN=NT26_4047 PE=4 SV=1
MM1 pKa = 6.75PTCGRR6 pKa = 11.84CLATRR11 pKa = 11.84LRR13 pKa = 11.84CRR15 pKa = 11.84RR16 pKa = 11.84LKK18 pKa = 10.22PLPPRR23 pKa = 11.84VKK25 pKa = 10.38PLLPLLKK32 pKa = 10.56VLLLRR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.59LLLRR43 pKa = 11.84RR44 pKa = 11.84TLLPLAKK51 pKa = 9.9VKK53 pKa = 10.35PLLRR57 pKa = 11.84PRR59 pKa = 11.84LLPLLLAKK67 pKa = 10.34RR68 pKa = 11.84PQPKK72 pKa = 9.36VQHH75 pKa = 5.93LQPKK79 pKa = 9.46RR80 pKa = 11.84RR81 pKa = 11.84PLLPRR86 pKa = 11.84KK87 pKa = 9.06SQHH90 pKa = 5.32RR91 pKa = 3.73

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4603

0

4603

1374660

12

2835

298.6

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.794 ± 0.045

0.836 ± 0.01

5.622 ± 0.027

6.162 ± 0.038

3.792 ± 0.025

8.389 ± 0.032

2.047 ± 0.018

5.478 ± 0.025

3.375 ± 0.036

10.083 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.016

2.662 ± 0.022

4.975 ± 0.026

3.165 ± 0.02

7.068 ± 0.033

5.695 ± 0.024

5.253 ± 0.023

7.411 ± 0.028

1.267 ± 0.015

2.264 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski