Cleome leaf crumple virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9CLW2|G9CLW2_9GEMI Nuclear shuttle protein OS=Cleome leaf crumple virus OX=666144 GN=BV1 PE=3 SV=1
MM1 pKa = 7.67PSAPKK6 pKa = 10.26RR7 pKa = 11.84FLINARR13 pKa = 11.84NYY15 pKa = 9.17FLTYY19 pKa = 9.16PKK21 pKa = 10.53CSLTKK26 pKa = 10.69EE27 pKa = 4.09EE28 pKa = 5.46ALSQLQALATPTNKK42 pKa = 9.94KK43 pKa = 9.17FIKK46 pKa = 9.73VARR49 pKa = 11.84EE50 pKa = 3.55LHH52 pKa = 7.09DD53 pKa = 3.79NGEE56 pKa = 4.18PHH58 pKa = 6.94LHH60 pKa = 6.13VLLQFEE66 pKa = 5.47GKK68 pKa = 10.02FKK70 pKa = 10.81CQNQRR75 pKa = 11.84FFDD78 pKa = 3.97LVSPSRR84 pKa = 11.84SAHH87 pKa = 5.01FHH89 pKa = 6.64PNVQGAKK96 pKa = 9.76SSSDD100 pKa = 3.1VKK102 pKa = 11.22SYY104 pKa = 11.11VDD106 pKa = 3.65KK107 pKa = 11.62DD108 pKa = 3.31GDD110 pKa = 3.81TLEE113 pKa = 4.43WGTFQIDD120 pKa = 3.03GRR122 pKa = 11.84SARR125 pKa = 11.84GGCQSANDD133 pKa = 4.0TYY135 pKa = 11.84AKK137 pKa = 10.37VLNAEE142 pKa = 4.28SAAQALQILRR152 pKa = 11.84EE153 pKa = 3.98EE154 pKa = 4.19QPRR157 pKa = 11.84DD158 pKa = 3.57FVLHH162 pKa = 6.37LDD164 pKa = 3.37KK165 pKa = 11.46VQAHH169 pKa = 4.77VQKK172 pKa = 10.56IFAKK176 pKa = 10.48APEE179 pKa = 4.24PWVPPFPLSSFTNVPDD195 pKa = 4.91EE196 pKa = 4.21MQSWADD202 pKa = 3.48EE203 pKa = 4.3YY204 pKa = 11.24FALSAEE210 pKa = 4.12ARR212 pKa = 11.84PNRR215 pKa = 11.84PISLIVEE222 pKa = 4.55GDD224 pKa = 3.35SRR226 pKa = 11.84TGKK229 pKa = 8.55TMWARR234 pKa = 11.84ALGPHH239 pKa = 6.63NYY241 pKa = 10.14LSGHH245 pKa = 6.9LDD247 pKa = 3.48FNSRR251 pKa = 11.84VYY253 pKa = 11.14SNEE256 pKa = 3.07VAYY259 pKa = 10.9NVIDD263 pKa = 4.3DD264 pKa = 4.17VAPHH268 pKa = 5.29YY269 pKa = 10.97LKK271 pKa = 10.78LKK273 pKa = 9.04HH274 pKa = 5.8WKK276 pKa = 9.61EE277 pKa = 3.74LVGAQRR283 pKa = 11.84DD284 pKa = 3.66WQSNCKK290 pKa = 9.41YY291 pKa = 10.21GKK293 pKa = 9.18PVQIKK298 pKa = 10.19GGIPSIVLCNPGEE311 pKa = 4.21GSSYY315 pKa = 11.31KK316 pKa = 10.75DD317 pKa = 3.23FLEE320 pKa = 5.14KK321 pKa = 10.95EE322 pKa = 4.28EE323 pKa = 4.19NSALRR328 pKa = 11.84SWTLHH333 pKa = 4.72NARR336 pKa = 11.84FVFLEE341 pKa = 4.03SPLYY345 pKa = 10.6QSATQSGEE353 pKa = 4.35TEE355 pKa = 4.08SHH357 pKa = 6.32SPP359 pKa = 3.4

Molecular weight: 40.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8G2J3|G8G2J3_9GEMI Replication enhancer OS=Cleome leaf crumple virus OX=666144 GN=AC3 PE=3 SV=1
MM1 pKa = 7.59GSLISTCFSNSRR13 pKa = 11.84GSSNARR19 pKa = 11.84IKK21 pKa = 10.88DD22 pKa = 3.23SSTWYY27 pKa = 9.18PRR29 pKa = 11.84PGQHH33 pKa = 6.05ISIRR37 pKa = 11.84TYY39 pKa = 10.73RR40 pKa = 11.84EE41 pKa = 3.4LSRR44 pKa = 11.84APTSSPTSTKK54 pKa = 10.38TGILWNGEE62 pKa = 3.97HH63 pKa = 6.36SRR65 pKa = 11.84STGEE69 pKa = 3.82VLEE72 pKa = 4.56AVANRR77 pKa = 11.84LTTHH81 pKa = 5.93TPKK84 pKa = 10.8SLTPSLQRR92 pKa = 11.84KK93 pKa = 7.52HH94 pKa = 6.62CRR96 pKa = 11.84FF97 pKa = 3.62

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1517

97

359

216.7

24.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.933 ± 0.675

1.846 ± 0.252

5.01 ± 0.55

4.483 ± 0.475

4.153 ± 0.326

5.405 ± 0.61

3.691 ± 0.121

4.68 ± 0.562

5.471 ± 0.403

7.581 ± 0.569

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.912 ± 0.392

5.142 ± 0.615

5.339 ± 0.548

3.955 ± 0.503

8.174 ± 1.032

9.558 ± 0.977

5.339 ± 0.807

6.922 ± 0.664

1.582 ± 0.18

3.823 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski