Lake Sarah-associated circular virus-47

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 9.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GAF5|A0A126GAF5_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-47 OX=1685776 PE=4 SV=1
MM1 pKa = 7.29RR2 pKa = 11.84QSKK5 pKa = 9.83GRR7 pKa = 11.84AQGGVKK13 pKa = 10.09NRR15 pKa = 11.84TSGATQSRR23 pKa = 11.84KK24 pKa = 9.87GKK26 pKa = 10.51AIDD29 pKa = 3.54AVVSLRR35 pKa = 11.84QASRR39 pKa = 11.84RR40 pKa = 11.84QNQVIDD46 pKa = 3.5ATSAYY51 pKa = 8.61MLYY54 pKa = 9.54SAPMRR59 pKa = 11.84RR60 pKa = 11.84TDD62 pKa = 4.6NKK64 pKa = 10.38FFDD67 pKa = 3.49PWVANGLKK75 pKa = 10.08PIIYY79 pKa = 8.21STEE82 pKa = 4.01GAVSNSAGGYY92 pKa = 8.42VLNTAATPSACVINQIPQGTTTNSRR117 pKa = 11.84LGRR120 pKa = 11.84KK121 pKa = 8.94ARR123 pKa = 11.84ITGVYY128 pKa = 9.31IKK130 pKa = 10.76GVVQSPGAVAAAAKK144 pKa = 7.87CTLALVHH151 pKa = 6.32QLPPNNATQMPAFTDD166 pKa = 2.45IWTAQHH172 pKa = 6.84CSAQRR177 pKa = 11.84NVDD180 pKa = 3.53NNDD183 pKa = 2.8KK184 pKa = 10.77FKK186 pKa = 10.86VIRR189 pKa = 11.84AITVDD194 pKa = 3.54CMGNAAAPATGKK206 pKa = 10.27EE207 pKa = 4.37LINLDD212 pKa = 4.01EE213 pKa = 4.9MIDD216 pKa = 3.73LKK218 pKa = 11.04GKK220 pKa = 10.06EE221 pKa = 4.93IITEE225 pKa = 3.94WTAADD230 pKa = 3.58TTGVYY235 pKa = 11.25GNMEE239 pKa = 4.36RR240 pKa = 11.84GALHH244 pKa = 7.36LYY246 pKa = 10.61ALANVPFATGYY257 pKa = 9.8VFQGTVRR264 pKa = 11.84VYY266 pKa = 10.87FEE268 pKa = 5.88DD269 pKa = 4.18YY270 pKa = 11.22

Molecular weight: 29.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GAF5|A0A126GAF5_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-47 OX=1685776 PE=4 SV=1
MM1 pKa = 7.56IFGAFLTGRR10 pKa = 11.84GPQSTHH16 pKa = 3.75WCFTVHH22 pKa = 6.81RR23 pKa = 11.84PQRR26 pKa = 11.84RR27 pKa = 11.84PAVLPEE33 pKa = 3.85SCQYY37 pKa = 11.4LVFQIEE43 pKa = 4.0KK44 pKa = 7.77TAQGKK49 pKa = 8.45RR50 pKa = 11.84HH51 pKa = 4.55VQAYY55 pKa = 7.15VQFPVRR61 pKa = 11.84TRR63 pKa = 11.84GTTVSSLAKK72 pKa = 9.85RR73 pKa = 11.84IFGGEE78 pKa = 3.85PSHH81 pKa = 6.81NEE83 pKa = 3.53PAKK86 pKa = 10.98GSDD89 pKa = 3.4QEE91 pKa = 4.41NEE93 pKa = 4.38VYY95 pKa = 8.86CTKK98 pKa = 10.31EE99 pKa = 3.69ASRR102 pKa = 11.84LEE104 pKa = 4.32GPWRR108 pKa = 11.84YY109 pKa = 10.11GEE111 pKa = 4.26RR112 pKa = 11.84VPHH115 pKa = 6.44AGKK118 pKa = 10.52KK119 pKa = 9.9GGRR122 pKa = 11.84SDD124 pKa = 4.68LLKK127 pKa = 11.18LKK129 pKa = 11.03DD130 pKa = 4.03NVDD133 pKa = 3.34KK134 pKa = 11.24GATEE138 pKa = 3.92LALFEE143 pKa = 4.18QQFGDD148 pKa = 3.59MVRR151 pKa = 11.84HH152 pKa = 5.82HH153 pKa = 6.83RR154 pKa = 11.84AIGHH158 pKa = 4.42YY159 pKa = 9.65RR160 pKa = 11.84YY161 pKa = 10.41LKK163 pKa = 10.72FNVEE167 pKa = 4.31RR168 pKa = 11.84HH169 pKa = 4.98WEE171 pKa = 4.26TIVILIVGPTNTQKK185 pKa = 9.48TFLSEE190 pKa = 3.64VLARR194 pKa = 11.84SGYY197 pKa = 9.4FGKK200 pKa = 8.53TVWRR204 pKa = 11.84APEE207 pKa = 4.17SKK209 pKa = 10.6GSGMYY214 pKa = 9.57FDD216 pKa = 5.57GYY218 pKa = 10.93NGHH221 pKa = 6.63EE222 pKa = 4.26CVLWQDD228 pKa = 3.96FDD230 pKa = 4.09GGSCKK235 pKa = 10.49FGTFKK240 pKa = 11.16NLIQGTPTNVPVHH253 pKa = 6.12GAASIEE259 pKa = 3.89WAPRR263 pKa = 11.84VMILTSNYY271 pKa = 9.34LPKK274 pKa = 10.49YY275 pKa = 6.67WWKK278 pKa = 10.05SHH280 pKa = 5.88QGANDD285 pKa = 3.56LAAIYY290 pKa = 10.22KK291 pKa = 9.34RR292 pKa = 11.84VHH294 pKa = 6.46VIFKK298 pKa = 10.2RR299 pKa = 11.84LKK301 pKa = 9.18PVEE304 pKa = 3.97NMPRR308 pKa = 11.84VAPIFHH314 pKa = 7.3PASLYY319 pKa = 10.08PPRR322 pKa = 11.84PDD324 pKa = 3.27PPLAVKK330 pKa = 9.86PLAISKK336 pKa = 10.11SKK338 pKa = 10.99KK339 pKa = 10.17LFNN342 pKa = 4.36

Molecular weight: 38.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

612

270

342

306.0

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.641 ± 1.97

1.471 ± 0.006

3.595 ± 0.453

4.085 ± 0.796

4.085 ± 0.796

8.333 ± 0.099

2.778 ± 0.889

4.739 ± 0.436

6.373 ± 0.633

6.209 ± 0.546

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.961 ± 0.337

4.739 ± 0.831

5.882 ± 0.965

4.575 ± 0.325

6.373 ± 0.238

5.392 ± 0.11

6.699 ± 0.971

7.516 ± 0.139

1.797 ± 0.366

3.758 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski