Senegalimassilia anaerobia

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369LCJ5|A0A369LCJ5_9ACTN Undecaprenyl-diphosphatase OS=Senegalimassilia anaerobia OX=1473216 GN=uppP PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.37SYY4 pKa = 10.8IEE6 pKa = 4.32PLNAADD12 pKa = 5.21ALDD15 pKa = 3.7VLDD18 pKa = 5.74LYY20 pKa = 11.56GIDD23 pKa = 3.43TPGAFFSSEE32 pKa = 3.96HH33 pKa = 5.9EE34 pKa = 4.33GCVEE38 pKa = 4.03VEE40 pKa = 4.27LSGHH44 pKa = 5.04TALLEE49 pKa = 4.36TPEE52 pKa = 4.17SVAMLFGHH60 pKa = 7.42DD61 pKa = 4.21AATALCQHH69 pKa = 6.27SNFRR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.01AAEE78 pKa = 3.8IMSRR82 pKa = 11.84ALDD85 pKa = 4.23LDD87 pKa = 3.96DD88 pKa = 5.35FLYY91 pKa = 10.93NVYY94 pKa = 10.48SGTAFIPDD102 pKa = 3.83KK103 pKa = 11.31APDD106 pKa = 3.65GEE108 pKa = 4.42FFGFYY113 pKa = 9.34VAHH116 pKa = 6.91IPLSAILEE124 pKa = 4.3DD125 pKa = 6.15DD126 pKa = 4.37IDD128 pKa = 4.31PLSPDD133 pKa = 3.06ACDD136 pKa = 3.65YY137 pKa = 11.37GLDD140 pKa = 4.19SISEE144 pKa = 4.34TTEE147 pKa = 3.86DD148 pKa = 3.39AAVGYY153 pKa = 8.96FDD155 pKa = 4.84PAGAVDD161 pKa = 4.72DD162 pKa = 4.89EE163 pKa = 4.85KK164 pKa = 11.33FLTSADD170 pKa = 3.22WALCDD175 pKa = 3.99IEE177 pKa = 5.94SSVLTAFGAGCSPEE191 pKa = 4.0LAGWTLGNAEE201 pKa = 4.75SVAEE205 pKa = 4.37TCASLGVDD213 pKa = 3.37IATAPAPDD221 pKa = 3.5NPARR225 pKa = 11.84VAAAAKK231 pKa = 9.87AATKK235 pKa = 10.37AATHH239 pKa = 6.17GVAAAAGNRR248 pKa = 11.84LL249 pKa = 3.39

Molecular weight: 25.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369L275|A0A369L275_9ACTN Energy-coupling factor transporter transmembrane protein EcfT OS=Senegalimassilia anaerobia OX=1473216 GN=C1880_09410 PE=4 SV=1
MM1 pKa = 7.36SVNGDD6 pKa = 3.37AARR9 pKa = 11.84RR10 pKa = 11.84DD11 pKa = 3.33MWAGRR16 pKa = 11.84IEE18 pKa = 4.21RR19 pKa = 11.84CLAADD24 pKa = 3.44MTVKK28 pKa = 10.17EE29 pKa = 4.26WCALNKK35 pKa = 9.9VAEE38 pKa = 4.18SSLYY42 pKa = 10.22KK43 pKa = 9.62WMARR47 pKa = 11.84FRR49 pKa = 11.84EE50 pKa = 4.32EE51 pKa = 3.75EE52 pKa = 4.13PRR54 pKa = 11.84PFPCRR59 pKa = 11.84SSEE62 pKa = 3.77ASSWMRR68 pKa = 11.84VARR71 pKa = 11.84RR72 pKa = 11.84GPGRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84CKK80 pKa = 9.11PVLRR84 pKa = 11.84SRR86 pKa = 11.84YY87 pKa = 8.79GG88 pKa = 3.06

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1843

0

1843

646842

37

4846

351.0

38.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.086 ± 0.085

1.692 ± 0.032

6.18 ± 0.057

6.311 ± 0.062

3.723 ± 0.04

8.356 ± 0.051

1.853 ± 0.028

4.872 ± 0.051

4.004 ± 0.052

8.803 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.036

3.016 ± 0.038

4.35 ± 0.039

3.378 ± 0.036

5.76 ± 0.065

5.833 ± 0.058

5.23 ± 0.058

8.023 ± 0.053

1.059 ± 0.023

2.751 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski