Roseobacter phage RDJL Phi 2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Xiamenvirus; Roseobacter virus RDJL2

Average proteome isoelectric point is 5.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0PVF9|A0A0K0PVF9_9CAUD Uncharacterized protein OS=Roseobacter phage RDJL Phi 2 OX=1682380 GN=RDJLphi2_gp21 PE=4 SV=1
MM1 pKa = 7.6TDD3 pKa = 3.17NIGTHH8 pKa = 5.9ARR10 pKa = 11.84AILDD14 pKa = 3.77TVDD17 pKa = 5.2AFIDD21 pKa = 3.65TCPDD25 pKa = 3.33LEE27 pKa = 4.32GLGIEE32 pKa = 4.12VGEE35 pKa = 4.12PFEE38 pKa = 5.0FDD40 pKa = 3.64TVPHH44 pKa = 6.64GLRR47 pKa = 11.84SEE49 pKa = 4.14VTIDD53 pKa = 3.21GVLYY57 pKa = 10.47AVTVEE62 pKa = 4.26PCHH65 pKa = 6.85DD66 pKa = 3.4

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0PVI6|A0A0K0PVI6_9CAUD Membrane spanning protein TolA OS=Roseobacter phage RDJL Phi 2 OX=1682380 GN=RDJLphi2_gp40 PE=4 SV=1
MM1 pKa = 6.5TTPIRR6 pKa = 11.84MTSSQIRR13 pKa = 11.84KK14 pKa = 8.44LQRR17 pKa = 11.84QLNRR21 pKa = 11.84LGYY24 pKa = 10.64GPLDD28 pKa = 3.25VDD30 pKa = 3.95GVYY33 pKa = 10.82GPKK36 pKa = 9.44TKK38 pKa = 10.02RR39 pKa = 11.84AHH41 pKa = 5.71TKK43 pKa = 8.66ATNDD47 pKa = 3.24RR48 pKa = 11.84NGSDD52 pKa = 3.23RR53 pKa = 11.84QFQRR57 pKa = 11.84PRR59 pKa = 11.84THH61 pKa = 7.12VIHH64 pKa = 6.15ATDD67 pKa = 3.4WPINSQQDD75 pKa = 3.96LIAFYY80 pKa = 10.21GSPGGPQCTAGKK92 pKa = 10.24VILPFPFKK100 pKa = 10.55IAWDD104 pKa = 3.72LDD106 pKa = 3.51KK107 pKa = 11.31SVSRR111 pKa = 11.84FSCHH115 pKa = 6.8DD116 pKa = 3.39KK117 pKa = 10.78VVQPMTGIFRR127 pKa = 11.84DD128 pKa = 3.69AAAHH132 pKa = 5.28YY133 pKa = 10.66GEE135 pKa = 4.94AEE137 pKa = 4.04FRR139 pKa = 11.84RR140 pKa = 11.84LGLDD144 pKa = 4.01LFGGCYY150 pKa = 9.27NNRR153 pKa = 11.84AIRR156 pKa = 11.84GGSRR160 pKa = 11.84KK161 pKa = 8.24STHH164 pKa = 5.35AWGIAVDD171 pKa = 4.96LDD173 pKa = 3.65PARR176 pKa = 11.84NRR178 pKa = 11.84LRR180 pKa = 11.84WGRR183 pKa = 11.84DD184 pKa = 2.56RR185 pKa = 11.84AVFARR190 pKa = 11.84PEE192 pKa = 4.11YY193 pKa = 10.37EE194 pKa = 4.1PFWQIVEE201 pKa = 4.62AYY203 pKa = 9.13GATSLGRR210 pKa = 11.84AANFDD215 pKa = 3.12WMHH218 pKa = 6.03FQFADD223 pKa = 3.66QII225 pKa = 3.98

Molecular weight: 25.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

18117

51

1360

238.4

26.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.14 ± 0.491

0.971 ± 0.133

7.22 ± 0.239

7.424 ± 0.32

3.649 ± 0.172

7.992 ± 0.291

1.855 ± 0.172

4.565 ± 0.155

4.763 ± 0.355

8.368 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.561 ± 0.196

3.483 ± 0.193

4.515 ± 0.215

3.411 ± 0.172

6.182 ± 0.282

5.492 ± 0.378

6.557 ± 0.337

6.403 ± 0.204

1.91 ± 0.179

2.539 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski