Ezakiella coagulans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellia incertae sedis; Ezakiella

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1E2J3|A0A2U1E2J3_9FIRM Branched-chain amino acid aminotransferase OS=Ezakiella coagulans OX=46507 GN=C7381_10854 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 3.54EE3 pKa = 4.68HH4 pKa = 6.93KK5 pKa = 10.56KK6 pKa = 9.39HH7 pKa = 6.82HH8 pKa = 6.78NDD10 pKa = 4.04DD11 pKa = 3.77EE12 pKa = 4.79HH13 pKa = 7.06EE14 pKa = 4.44CTCGCGCGCHH24 pKa = 6.98DD25 pKa = 4.34EE26 pKa = 5.46DD27 pKa = 5.86EE28 pKa = 4.39MVEE31 pKa = 4.01YY32 pKa = 8.9DD33 pKa = 3.62TVNITFEE40 pKa = 4.35DD41 pKa = 4.05DD42 pKa = 3.91KK43 pKa = 11.53EE44 pKa = 4.51SEE46 pKa = 4.47CAVLDD51 pKa = 3.52QFKK54 pKa = 10.45IGEE57 pKa = 4.23KK58 pKa = 10.12EE59 pKa = 4.16YY60 pKa = 10.56MVLLPLEE67 pKa = 4.82DD68 pKa = 3.91NEE70 pKa = 4.41EE71 pKa = 4.07EE72 pKa = 3.77LVYY75 pKa = 10.19IYY77 pKa = 10.43RR78 pKa = 11.84YY79 pKa = 8.4TDD81 pKa = 3.47DD82 pKa = 3.9EE83 pKa = 4.66NGSFTLDD90 pKa = 3.54VIEE93 pKa = 6.36DD94 pKa = 3.81EE95 pKa = 5.33DD96 pKa = 4.64EE97 pKa = 4.59LDD99 pKa = 3.78EE100 pKa = 4.62VCDD103 pKa = 3.79KK104 pKa = 11.3FEE106 pKa = 4.34EE107 pKa = 4.25RR108 pKa = 11.84AEE110 pKa = 4.14LNEE113 pKa = 4.13EE114 pKa = 4.07

Molecular weight: 13.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1E5J1|A0A2U1E5J1_9FIRM Phosphoglycerol transferase MdoB-like AlkP superfamily enzyme OS=Ezakiella coagulans OX=46507 GN=C7381_102105 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.68QPKK8 pKa = 8.57NRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 7.76KK14 pKa = 7.74VHH16 pKa = 5.67GFRR19 pKa = 11.84EE20 pKa = 3.96RR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.51NGRR28 pKa = 11.84NVLKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.85VLSAA44 pKa = 4.11

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1626

0

1626

533091

27

4123

327.9

37.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.801 ± 0.063

0.811 ± 0.027

6.426 ± 0.054

7.88 ± 0.061

4.802 ± 0.045

6.421 ± 0.057

1.358 ± 0.024

8.8 ± 0.078

9.093 ± 0.066

8.839 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.038

5.645 ± 0.058

2.996 ± 0.048

2.075 ± 0.032

3.916 ± 0.045

5.983 ± 0.053

5.092 ± 0.064

6.62 ± 0.053

0.606 ± 0.018

4.084 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski