Reinekea marinisedimentorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Saccharospirillaceae; Reinekea

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3HU06|A0A4R3HU06_9GAMM Peptide chain release factor 3 OS=Reinekea marinisedimentorum OX=230495 GN=prfC PE=3 SV=1
MM1 pKa = 8.05PLTQFKK7 pKa = 10.49RR8 pKa = 11.84KK9 pKa = 9.24ALVVALVSQAGLTATNVAVANDD31 pKa = 3.58STEE34 pKa = 4.02IEE36 pKa = 4.11TDD38 pKa = 3.52TIVISGEE45 pKa = 3.86KK46 pKa = 9.38IEE48 pKa = 5.03RR49 pKa = 11.84SLKK52 pKa = 9.75EE53 pKa = 3.42ATTAVTVITEE63 pKa = 4.36DD64 pKa = 3.31QLQTTEE70 pKa = 4.01TQTINEE76 pKa = 4.38IAASTPNVINGGFGAVSIRR95 pKa = 11.84GVDD98 pKa = 3.48GTGAATGGYY107 pKa = 9.96AFYY110 pKa = 10.53SGARR114 pKa = 11.84ARR116 pKa = 11.84VSTIVDD122 pKa = 3.64GVSQSWSGYY131 pKa = 9.2NFTPSKK137 pKa = 10.44SWDD140 pKa = 3.41VKK142 pKa = 9.5QVEE145 pKa = 5.28VYY147 pKa = 10.44RR148 pKa = 11.84GPQSTTQGTNSIGGAMVVEE167 pKa = 4.96SNDD170 pKa = 3.03PTYY173 pKa = 10.57YY174 pKa = 10.2QEE176 pKa = 3.76AAIRR180 pKa = 11.84LGTEE184 pKa = 3.92VYY186 pKa = 10.79EE187 pKa = 4.69NGNTLSNLAVMASGPLIEE205 pKa = 5.86DD206 pKa = 3.21EE207 pKa = 4.39LAFRR211 pKa = 11.84LAVDD215 pKa = 4.33GSTGEE220 pKa = 4.12SYY222 pKa = 8.67ITYY225 pKa = 9.0EE226 pKa = 4.01QAATEE231 pKa = 4.59LDD233 pKa = 4.01DD234 pKa = 6.71SPDD237 pKa = 3.62LDD239 pKa = 3.46NTTNLNLRR247 pKa = 11.84SKK249 pKa = 10.49LLWEE253 pKa = 4.77PGFLPEE259 pKa = 5.01LSAKK263 pKa = 9.37ATVNYY268 pKa = 10.59SSFDD272 pKa = 3.29GSYY275 pKa = 11.06LNWANDD281 pKa = 3.19TDD283 pKa = 4.24EE284 pKa = 5.95DD285 pKa = 5.17YY286 pKa = 10.78STQTFTVSSADD297 pKa = 3.45RR298 pKa = 11.84DD299 pKa = 3.67YY300 pKa = 11.81TRR302 pKa = 11.84IQDD305 pKa = 3.72STVKK309 pKa = 10.51SVAGDD314 pKa = 3.46ADD316 pKa = 3.91YY317 pKa = 11.52QLTDD321 pKa = 3.38QISNSFQIVYY331 pKa = 10.81AEE333 pKa = 3.87TDD335 pKa = 3.56VEE337 pKa = 4.45FNEE340 pKa = 4.54YY341 pKa = 9.42TSSRR345 pKa = 11.84TVISDD350 pKa = 3.15VDD352 pKa = 3.58NVTVEE357 pKa = 3.51NRR359 pKa = 11.84LNFRR363 pKa = 11.84ASDD366 pKa = 3.7DD367 pKa = 3.65SATAFVGVYY376 pKa = 10.33LSNTEE381 pKa = 3.87TTQDD385 pKa = 3.12IFSVIDD391 pKa = 3.44VEE393 pKa = 5.21SDD395 pKa = 3.28VTTAAVFGEE404 pKa = 4.34ASYY407 pKa = 11.37QLLDD411 pKa = 4.07EE412 pKa = 5.12LSLIAGLRR420 pKa = 11.84YY421 pKa = 10.0EE422 pKa = 4.5NEE424 pKa = 4.01STDD427 pKa = 3.67RR428 pKa = 11.84MLDD431 pKa = 3.2HH432 pKa = 7.6ASNTNYY438 pKa = 10.33NFDD441 pKa = 3.62EE442 pKa = 4.52SVSEE446 pKa = 4.95DD447 pKa = 3.38VLLPKK452 pKa = 10.16IAAIYY457 pKa = 9.65EE458 pKa = 4.28LTDD461 pKa = 3.32NTVVSASVRR470 pKa = 11.84KK471 pKa = 9.91GYY473 pKa = 9.88NAGGGAVNWASDD485 pKa = 3.56TDD487 pKa = 3.93NIGYY491 pKa = 7.58YY492 pKa = 9.68TYY494 pKa = 11.1DD495 pKa = 3.53SEE497 pKa = 5.01TVWAYY502 pKa = 9.62EE503 pKa = 3.95AALKK507 pKa = 10.1TSIAGANVAVSAFINDD523 pKa = 3.51YY524 pKa = 11.33ADD526 pKa = 3.31YY527 pKa = 10.49QAFVSEE533 pKa = 4.57YY534 pKa = 10.77DD535 pKa = 3.35SSLDD539 pKa = 3.42DD540 pKa = 3.32TLQYY544 pKa = 10.44IEE546 pKa = 4.59NVEE549 pKa = 4.1DD550 pKa = 3.63ALTYY554 pKa = 10.36GVEE557 pKa = 4.1LEE559 pKa = 4.02GDD561 pKa = 3.09KK562 pKa = 10.67WLTDD566 pKa = 3.63STSLRR571 pKa = 11.84ASVGYY576 pKa = 7.78TQTEE580 pKa = 4.65VISDD584 pKa = 4.14DD585 pKa = 3.6DD586 pKa = 4.48TIDD589 pKa = 4.25GNEE592 pKa = 4.61LPNAPAFNTSVGVTQYY608 pKa = 10.78FSDD611 pKa = 4.01RR612 pKa = 11.84FSVGADD618 pKa = 2.97VVYY621 pKa = 10.84VGEE624 pKa = 4.51YY625 pKa = 10.92YY626 pKa = 10.81SDD628 pKa = 4.17LDD630 pKa = 3.53NTSDD634 pKa = 3.86YY635 pKa = 11.22KK636 pKa = 11.35AGDD639 pKa = 3.77YY640 pKa = 8.55VTADD644 pKa = 2.6IRR646 pKa = 11.84ANYY649 pKa = 9.6VIGDD653 pKa = 4.22FTLDD657 pKa = 3.25AYY659 pKa = 11.03VKK661 pKa = 10.58NLTNEE666 pKa = 4.79DD667 pKa = 2.66IVYY670 pKa = 9.06WDD672 pKa = 3.21NGAIEE677 pKa = 4.35RR678 pKa = 11.84VSVGQTRR685 pKa = 11.84TIGFNATFRR694 pKa = 11.84MM695 pKa = 4.24

Molecular weight: 75.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3HX17|A0A4R3HX17_9GAMM Transposase IS166 family protein OS=Reinekea marinisedimentorum OX=230495 GN=BCF53_12329 PE=4 SV=1
MM1 pKa = 7.19NRR3 pKa = 11.84ASNATIPGRR12 pKa = 11.84TWVKK16 pKa = 9.23RR17 pKa = 11.84TFYY20 pKa = 11.06LNRR23 pKa = 11.84SSLNDD28 pKa = 3.62MNHH31 pKa = 4.45VTQTLDD37 pKa = 3.08SALGIHH43 pKa = 6.4SVTFMPIQNSIEE55 pKa = 4.21VVFDD59 pKa = 3.58ARR61 pKa = 11.84LTCSQQIVAIFQQHH75 pKa = 6.37HH76 pKa = 6.06LPLRR80 pKa = 11.84AGLKK84 pKa = 10.16DD85 pKa = 3.26RR86 pKa = 11.84VRR88 pKa = 11.84FAWYY92 pKa = 10.09RR93 pKa = 11.84FIEE96 pKa = 4.18QNIKK100 pKa = 10.92DD101 pKa = 4.12NEE103 pKa = 4.26SSSRR107 pKa = 11.84WKK109 pKa = 10.25PLTPARR115 pKa = 4.25

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3800

0

3800

1253563

29

2328

329.9

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.194 ± 0.04

1.031 ± 0.015

5.659 ± 0.046

6.583 ± 0.038

4.102 ± 0.027

6.89 ± 0.033

2.122 ± 0.019

6.105 ± 0.028

4.757 ± 0.032

10.376 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.018

4.108 ± 0.028

3.971 ± 0.027

4.386 ± 0.033

4.699 ± 0.032

6.795 ± 0.036

5.345 ± 0.029

6.979 ± 0.031

1.334 ± 0.016

3.088 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski