Vibrio phage Marilyn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9YZD6|A0A6M9YZD6_9CAUD Uncharacterized protein OS=Vibrio phage Marilyn OX=2736287 GN=MARILYN_24 PE=4 SV=1
MM1 pKa = 7.41SKK3 pKa = 8.1TWIAKK8 pKa = 9.13CVSFKK13 pKa = 10.88GKK15 pKa = 9.67HH16 pKa = 4.75PQGEE20 pKa = 4.34VTHH23 pKa = 6.14RR24 pKa = 11.84VVVKK28 pKa = 10.46AQYY31 pKa = 10.54KK32 pKa = 9.85KK33 pKa = 10.92DD34 pKa = 3.82GIEE37 pKa = 3.92AAQEE41 pKa = 3.82EE42 pKa = 5.0LKK44 pKa = 11.22LFDD47 pKa = 4.31VEE49 pKa = 5.25HH50 pKa = 6.6HH51 pKa = 7.13DD52 pKa = 4.01KK53 pKa = 11.07YY54 pKa = 11.13KK55 pKa = 10.76KK56 pKa = 10.08PEE58 pKa = 3.94LTEE61 pKa = 4.1VMDD64 pKa = 3.71EE65 pKa = 4.4SEE67 pKa = 4.37AEE69 pKa = 4.1RR70 pKa = 11.84IYY72 pKa = 10.51IEE74 pKa = 4.08YY75 pKa = 9.01MAVRR79 pKa = 11.84EE80 pKa = 3.83QLEE83 pKa = 4.12QIDD86 pKa = 4.29LCDD89 pKa = 4.95DD90 pKa = 3.47EE91 pKa = 6.59SNFNDD96 pKa = 3.52VEE98 pKa = 4.2QEE100 pKa = 3.84KK101 pKa = 10.05TDD103 pKa = 3.5AYY105 pKa = 10.63MEE107 pKa = 4.26ATQTLLARR115 pKa = 11.84YY116 pKa = 9.29DD117 pKa = 3.44IIDD120 pKa = 3.74RR121 pKa = 11.84VTVNHH126 pKa = 6.31IMEE129 pKa = 4.53IRR131 pKa = 11.84NKK133 pKa = 10.0LEE135 pKa = 3.73EE136 pKa = 4.23VIKK139 pKa = 10.67EE140 pKa = 4.07NEE142 pKa = 4.06EE143 pKa = 3.91CDD145 pKa = 4.52PIATLEE151 pKa = 4.52NIASYY156 pKa = 10.05NWKK159 pKa = 9.44QRR161 pKa = 11.84FPMGTDD167 pKa = 2.7EE168 pKa = 6.53KK169 pKa = 10.43MLQVLLNRR177 pKa = 11.84RR178 pKa = 11.84EE179 pKa = 3.67MRR181 pKa = 11.84AALDD185 pKa = 3.17GMYY188 pKa = 9.74IEE190 pKa = 4.81KK191 pKa = 8.09TTTEE195 pKa = 4.25EE196 pKa = 3.95PSEE199 pKa = 3.98EE200 pKa = 4.01LVDD203 pKa = 3.83SGEE206 pKa = 4.11KK207 pKa = 8.89FTAEE211 pKa = 3.78KK212 pKa = 9.74ATIAEE217 pKa = 4.16YY218 pKa = 9.42PIIEE222 pKa = 4.78PYY224 pKa = 9.67SVRR227 pKa = 11.84IAVAKK232 pKa = 9.61QGEE235 pKa = 4.72SFVHH239 pKa = 6.42SMAVVNRR246 pKa = 11.84EE247 pKa = 3.55KK248 pKa = 10.97GGNVYY253 pKa = 10.42GQLDD257 pKa = 3.76VFNPKK262 pKa = 10.06EE263 pKa = 3.9FTNVEE268 pKa = 4.06NAVVYY273 pKa = 10.23ALAMSIEE280 pKa = 4.35AVNKK284 pKa = 8.72YY285 pKa = 9.96LPHH288 pKa = 6.77FLEE291 pKa = 4.29EE292 pKa = 4.23RR293 pKa = 11.84QVNTCVNILSSYY305 pKa = 9.87AQDD308 pKa = 3.36AEE310 pKa = 4.65GNFIEE315 pKa = 6.26RR316 pKa = 11.84YY317 pKa = 9.21MGDD320 pKa = 3.51HH321 pKa = 7.26DD322 pKa = 5.27LSNATFMAVSQPKK335 pKa = 9.96EE336 pKa = 3.74EE337 pKa = 4.59SKK339 pKa = 10.74QSSGKK344 pKa = 10.03EE345 pKa = 3.83VTSDD349 pKa = 3.27VARR352 pKa = 11.84ITAAAVAHH360 pKa = 6.34PSIAPHH366 pKa = 6.2MEE368 pKa = 3.55TDD370 pKa = 3.22NRR372 pKa = 11.84AKK374 pKa = 10.13EE375 pKa = 3.99VYY377 pKa = 10.13YY378 pKa = 10.0QIQEE382 pKa = 4.15NFTALHH388 pKa = 5.83NKK390 pKa = 10.02YY391 pKa = 10.26GFLPAAQMVEE401 pKa = 4.74HH402 pKa = 7.47IISMSDD408 pKa = 3.13VSDD411 pKa = 5.28LITIEE416 pKa = 4.4GCDD419 pKa = 3.92EE420 pKa = 4.12LASHH424 pKa = 5.93YY425 pKa = 10.99VEE427 pKa = 4.24NTTTQTMQAITEE439 pKa = 4.2SSEE442 pKa = 3.82EE443 pKa = 3.97EE444 pKa = 3.99NDD446 pKa = 3.3VKK448 pKa = 10.84FYY450 pKa = 11.13AGQVWRR456 pKa = 11.84CYY458 pKa = 10.01IEE460 pKa = 5.41EE461 pKa = 4.02ILAQFKK467 pKa = 8.98TEE469 pKa = 3.99KK470 pKa = 10.3QISSEE475 pKa = 4.76DD476 pKa = 2.95IDD478 pKa = 4.22AAAKK482 pKa = 10.35NISRR486 pKa = 11.84LFNDD490 pKa = 3.32WKK492 pKa = 11.1LSLFKK497 pKa = 10.95EE498 pKa = 4.05KK499 pKa = 10.7GKK501 pKa = 8.92YY502 pKa = 8.72TIDD505 pKa = 3.27ADD507 pKa = 3.78MFKK510 pKa = 11.19DD511 pKa = 3.99EE512 pKa = 5.43IYY514 pKa = 10.92LIFNNSEE521 pKa = 3.88YY522 pKa = 10.84QKK524 pKa = 11.35ALEE527 pKa = 4.05LLKK530 pKa = 10.72DD531 pKa = 3.67YY532 pKa = 10.81FGSKK536 pKa = 10.01DD537 pKa = 3.28QLVNVLACGFDD548 pKa = 4.02DD549 pKa = 5.36YY550 pKa = 12.11VNEE553 pKa = 4.38LNPKK557 pKa = 9.47VKK559 pKa = 10.59EE560 pKa = 3.94EE561 pKa = 3.91LVNLVNRR568 pKa = 11.84LAVDD572 pKa = 3.71SSLGCTHH579 pKa = 6.64TSEE582 pKa = 4.43VVKK585 pKa = 10.86ALIYY589 pKa = 10.65SEE591 pKa = 5.35LSAEE595 pKa = 4.39SVPDD599 pKa = 3.6MVILKK604 pKa = 9.75PNLKK608 pKa = 9.22MDD610 pKa = 4.04FWNKK614 pKa = 9.87LNHH617 pKa = 7.13DD618 pKa = 3.87YY619 pKa = 11.12CSNQRR624 pKa = 11.84QRR626 pKa = 11.84QEE628 pKa = 4.06EE629 pKa = 4.55TVGDD633 pKa = 3.7YY634 pKa = 11.59VMAYY638 pKa = 10.02GSSDD642 pKa = 3.56EE643 pKa = 4.76LRR645 pKa = 11.84AKK647 pKa = 10.71ANEE650 pKa = 3.61IDD652 pKa = 4.08AKK654 pKa = 10.35AKK656 pKa = 9.97EE657 pKa = 4.5YY658 pKa = 10.54INEE661 pKa = 3.94VCEE664 pKa = 4.28PLAQALNEE672 pKa = 4.14KK673 pKa = 9.21VQEE676 pKa = 4.24VNAKK680 pKa = 10.11AEE682 pKa = 4.23EE683 pKa = 4.39YY684 pKa = 10.61EE685 pKa = 4.13NEE687 pKa = 3.87ACVRR691 pKa = 11.84GDD693 pKa = 3.16GVTPEE698 pKa = 4.35SEE700 pKa = 4.44RR701 pKa = 11.84FTIDD705 pKa = 3.4APKK708 pKa = 9.42TLPDD712 pKa = 3.67APEE715 pKa = 4.02LRR717 pKa = 11.84EE718 pKa = 3.85GATIGNSVEE727 pKa = 4.17ITDD730 pKa = 4.11EE731 pKa = 4.36EE732 pKa = 4.89YY733 pKa = 10.89SALLNPEE740 pKa = 4.83DD741 pKa = 3.87VPEE744 pKa = 4.05PQILPDD750 pKa = 3.89DD751 pKa = 3.79MEE753 pKa = 4.34VRR755 pKa = 11.84MEE757 pKa = 3.81EE758 pKa = 3.95TDD760 pKa = 2.97ASKK763 pKa = 11.39YY764 pKa = 10.8YY765 pKa = 9.49GTALLNGDD773 pKa = 3.21IDD775 pKa = 4.03SPYY778 pKa = 10.04EE779 pKa = 3.6LAIAYY784 pKa = 7.5MMKK787 pKa = 10.62DD788 pKa = 3.48LGAEE792 pKa = 4.14NYY794 pKa = 10.01HH795 pKa = 6.8DD796 pKa = 4.61AGWYY800 pKa = 9.28QYY802 pKa = 11.32AAAKK806 pKa = 10.24LRR808 pKa = 11.84GAAEE812 pKa = 4.08RR813 pKa = 11.84LKK815 pKa = 10.87CDD817 pKa = 4.45LPALMTGLTVEE828 pKa = 4.05KK829 pKa = 10.32LVRR832 pKa = 11.84EE833 pKa = 4.07KK834 pKa = 10.6SWANVEE840 pKa = 4.13EE841 pKa = 4.57LEE843 pKa = 4.54QALEE847 pKa = 3.97SHH849 pKa = 7.18EE850 pKa = 4.32ILDD853 pKa = 3.86TALYY857 pKa = 8.43EE858 pKa = 4.49HH859 pKa = 6.94GVFDD863 pKa = 4.04YY864 pKa = 11.32DD865 pKa = 3.85EE866 pKa = 4.51EE867 pKa = 4.77VLFGTDD873 pKa = 3.32HH874 pKa = 5.53QQEE877 pKa = 4.3APADD881 pKa = 3.9EE882 pKa = 4.84PEE884 pKa = 4.35EE885 pKa = 3.96ARR887 pKa = 11.84QDD889 pKa = 3.58IEE891 pKa = 4.73PDD893 pKa = 2.95NDD895 pKa = 4.1DD896 pKa = 4.48PVTSADD902 pKa = 3.82NGDD905 pKa = 4.25SVATDD910 pKa = 3.27NEE912 pKa = 4.23LSVRR916 pKa = 11.84EE917 pKa = 3.93LFKK920 pKa = 11.06RR921 pKa = 11.84QMAAANQKK929 pKa = 10.32LGLVGFAWGYY939 pKa = 6.51EE940 pKa = 4.38VKK942 pKa = 10.69RR943 pKa = 11.84QWTEE947 pKa = 3.53DD948 pKa = 3.5KK949 pKa = 10.95KK950 pKa = 11.04DD951 pKa = 3.26GRR953 pKa = 11.84TDD955 pKa = 3.11CHH957 pKa = 7.99VEE959 pKa = 3.59VGIWFKK965 pKa = 11.36DD966 pKa = 3.33KK967 pKa = 11.27NGTKK971 pKa = 10.43GEE973 pKa = 4.51PISFYY978 pKa = 11.04GVSNANFGNYY988 pKa = 9.18VNDD991 pKa = 3.32AMMLAVVNGLEE1002 pKa = 3.83ITLISLGVEE1011 pKa = 4.23VEE1013 pKa = 4.37GG1014 pKa = 5.11

Molecular weight: 114.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9YZF3|A0A6M9YZF3_9CAUD Uncharacterized protein OS=Vibrio phage Marilyn OX=2736287 GN=MARILYN_54 PE=4 SV=1
MM1 pKa = 7.17FLIGAGNAVLRR12 pKa = 11.84KK13 pKa = 10.09NIFTTSVAKK22 pKa = 10.44LHH24 pKa = 5.86FKK26 pKa = 10.5KK27 pKa = 10.76VLSNFGGQGAPVGNHH42 pKa = 4.87GRR44 pKa = 11.84LKK46 pKa = 10.37RR47 pKa = 11.84NYY49 pKa = 8.36EE50 pKa = 4.13FPHH53 pKa = 6.56PAHH56 pKa = 6.72HH57 pKa = 7.17PFAIPHH63 pKa = 5.77IWW65 pKa = 3.02

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13179

31

1375

227.2

25.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.992 ± 0.453

0.865 ± 0.122

6.237 ± 0.254

7.398 ± 0.458

3.65 ± 0.19

6.844 ± 0.372

1.874 ± 0.194

5.911 ± 0.246

6.806 ± 0.276

7.542 ± 0.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.198

5.145 ± 0.214

3.703 ± 0.211

3.991 ± 0.202

4.796 ± 0.312

6.571 ± 0.339

6.047 ± 0.263

6.252 ± 0.274

1.404 ± 0.115

3.392 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski