bacteria symbiont BFo2 of Frankliniella occidentalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; unclassified Enterobacterales

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2909 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J8YRJ1|A0A0J8YRJ1_9GAMM N-acetylmuramoyl-L-alanine amidase OS=bacteria symbiont BFo2 of Frankliniella occidentalis OX=1628856 GN=AI29_03280 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.19IGLFYY7 pKa = 10.96GSSTCYY13 pKa = 10.49TEE15 pKa = 3.81MAAEE19 pKa = 5.21KK20 pKa = 9.11IQEE23 pKa = 4.38FIGQEE28 pKa = 4.04MVTLHH33 pKa = 5.56NVKK36 pKa = 10.53DD37 pKa = 4.05EE38 pKa = 4.55PLSTMHH44 pKa = 7.08EE45 pKa = 4.24YY46 pKa = 11.19DD47 pKa = 4.37LLILGMPTWDD57 pKa = 3.35FGEE60 pKa = 4.31LQEE63 pKa = 5.13DD64 pKa = 5.06WEE66 pKa = 4.6AQWQTLADD74 pKa = 4.26LDD76 pKa = 4.19LSGKK80 pKa = 10.08IIALYY85 pKa = 10.91GMGDD89 pKa = 3.29QAGYY93 pKa = 9.76SEE95 pKa = 4.66WFLDD99 pKa = 4.0ALGMLHH105 pKa = 7.62DD106 pKa = 5.08LLLPKK111 pKa = 10.44GVSFVGYY118 pKa = 9.29WPITGYY124 pKa = 10.76EE125 pKa = 4.46FISQKK130 pKa = 10.61PLTADD135 pKa = 3.36GQHH138 pKa = 5.72FVGLALDD145 pKa = 4.34DD146 pKa = 4.75ANQFEE151 pKa = 4.7EE152 pKa = 4.13TDD154 pKa = 3.25EE155 pKa = 6.06RR156 pKa = 11.84IANWCEE162 pKa = 3.75QILGEE167 pKa = 4.53VAEE170 pKa = 4.57LLL172 pKa = 4.03

Molecular weight: 19.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J8YL66|A0A0J8YL66_9GAMM Uncharacterized protein (Fragment) OS=bacteria symbiont BFo2 of Frankliniella occidentalis OX=1628856 GN=AI29_06615 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2909

0

2909

883206

46

2676

303.6

33.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.445 ± 0.048

1.038 ± 0.016

5.058 ± 0.036

5.52 ± 0.052

3.778 ± 0.034

7.159 ± 0.04

2.434 ± 0.024

6.062 ± 0.032

4.227 ± 0.036

10.877 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.023

3.623 ± 0.034

4.401 ± 0.028

5.372 ± 0.045

5.31 ± 0.043

6.454 ± 0.04

5.697 ± 0.043

6.792 ± 0.035

1.433 ± 0.018

2.846 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski