Isoptericola dokdonensis DS-3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Isoptericola; Isoptericola dokdonensis

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3403 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161III5|A0A161III5_9MICO Uncharacterized protein OS=Isoptericola dokdonensis DS-3 OX=1300344 GN=I598_2183 PE=4 SV=1
MM1 pKa = 6.89PTLRR5 pKa = 11.84RR6 pKa = 11.84ALAATAALALPAVALTACSTDD27 pKa = 3.47GEE29 pKa = 4.97GTGSDD34 pKa = 3.96DD35 pKa = 3.81GTLQVLASFYY45 pKa = 9.4PLQYY49 pKa = 10.07VAEE52 pKa = 4.2QVGGDD57 pKa = 3.96LVSVDD62 pKa = 3.61NLTPPSAEE70 pKa = 3.84PHH72 pKa = 6.29DD73 pKa = 5.34LEE75 pKa = 5.13LAPAQVRR82 pKa = 11.84AVGDD86 pKa = 3.67ADD88 pKa = 3.76LVVYY92 pKa = 10.08QSGFQAAVDD101 pKa = 3.78EE102 pKa = 4.89AVDD105 pKa = 3.5ARR107 pKa = 11.84QPEE110 pKa = 4.55HH111 pKa = 7.28VVDD114 pKa = 3.83AADD117 pKa = 3.72VVEE120 pKa = 5.44LEE122 pKa = 4.32ASPGTVEE129 pKa = 5.45HH130 pKa = 6.91MDD132 pKa = 4.24DD133 pKa = 3.34EE134 pKa = 5.87HH135 pKa = 8.17EE136 pKa = 4.74GEE138 pKa = 4.3TAEE141 pKa = 4.23EE142 pKa = 4.23HH143 pKa = 6.97AEE145 pKa = 4.1HH146 pKa = 7.17ADD148 pKa = 3.55EE149 pKa = 5.47EE150 pKa = 4.8DD151 pKa = 3.86GHH153 pKa = 7.6DD154 pKa = 4.29HH155 pKa = 7.02GALDD159 pKa = 3.54PHH161 pKa = 6.79FWLDD165 pKa = 3.49PTLLEE170 pKa = 4.26PVADD174 pKa = 3.89AVADD178 pKa = 3.92EE179 pKa = 4.98LSAVDD184 pKa = 4.16PGNAATYY191 pKa = 9.59AANAEE196 pKa = 4.29ALTATLDD203 pKa = 4.19DD204 pKa = 4.27LDD206 pKa = 4.22ARR208 pKa = 11.84YY209 pKa = 7.96ATALEE214 pKa = 4.42PCAGQTLVTSHH225 pKa = 6.1TAFGYY230 pKa = 9.82LAEE233 pKa = 5.31RR234 pKa = 11.84YY235 pKa = 9.78DD236 pKa = 3.81LVQIGIAAIDD246 pKa = 4.1PEE248 pKa = 4.9AEE250 pKa = 4.02PSPARR255 pKa = 11.84LRR257 pKa = 11.84EE258 pKa = 3.97IGDD261 pKa = 3.51VVEE264 pKa = 5.01ANDD267 pKa = 3.32VQTIFTEE274 pKa = 4.54TLTSPKK280 pKa = 10.08VAEE283 pKa = 4.42TLASDD288 pKa = 4.34LGVATAVLDD297 pKa = 4.07PLEE300 pKa = 4.46GLSTEE305 pKa = 4.37AADD308 pKa = 4.51AGADD312 pKa = 3.68YY313 pKa = 10.81VAVMDD318 pKa = 5.58DD319 pKa = 3.99NLDD322 pKa = 3.75SLVTGLGCDD331 pKa = 3.22AA332 pKa = 5.43

Molecular weight: 34.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161II59|A0A161II59_9MICO HTH cro/C1-type domain-containing protein OS=Isoptericola dokdonensis DS-3 OX=1300344 GN=I598_0107 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3403

0

3403

1153230

29

2802

338.9

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.449 ± 0.067

0.542 ± 0.009

6.947 ± 0.039

5.265 ± 0.04

2.528 ± 0.022

9.5 ± 0.04

2.137 ± 0.022

2.633 ± 0.031

1.427 ± 0.028

10.124 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.537 ± 0.016

1.466 ± 0.025

5.948 ± 0.039

2.515 ± 0.02

7.776 ± 0.061

4.754 ± 0.035

6.554 ± 0.044

10.525 ± 0.053

1.576 ± 0.019

1.797 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski