Lake Sarah-associated circular virus-19

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9N6|A0A126G9N6_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-19 OX=1685745 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 6.29DD3 pKa = 3.89YY4 pKa = 11.69LLKK7 pKa = 10.68FCEE10 pKa = 4.2VLGKK14 pKa = 10.45KK15 pKa = 9.27SSKK18 pKa = 9.85SSKK21 pKa = 10.1EE22 pKa = 3.46ILGNTDD28 pKa = 3.13TKK30 pKa = 10.65ISKK33 pKa = 9.73QPSPAKK39 pKa = 10.05HH40 pKa = 5.6YY41 pKa = 11.1CFTLNNYY48 pKa = 9.78SNNEE52 pKa = 3.93LNIVPTSLGSVGHH65 pKa = 6.07YY66 pKa = 9.93IYY68 pKa = 10.79GFEE71 pKa = 4.24VGAEE75 pKa = 4.28GTPHH79 pKa = 5.53LQGYY83 pKa = 9.44IEE85 pKa = 4.73LHH87 pKa = 5.22HH88 pKa = 6.88KK89 pKa = 9.55MRR91 pKa = 11.84ITEE94 pKa = 4.15FKK96 pKa = 10.4KK97 pKa = 10.32IEE99 pKa = 4.22GLGRR103 pKa = 11.84THH105 pKa = 7.59FEE107 pKa = 3.91KK108 pKa = 10.96CKK110 pKa = 10.58GSQADD115 pKa = 3.47NLKK118 pKa = 9.77YY119 pKa = 10.09CRR121 pKa = 11.84KK122 pKa = 8.59GGNYY126 pKa = 9.09ISNYY130 pKa = 8.58WEE132 pKa = 4.25DD133 pKa = 3.38VQLIVPTYY141 pKa = 8.99YY142 pKa = 9.43WQLDD146 pKa = 3.62ILQVIASPAHH156 pKa = 5.84GRR158 pKa = 11.84HH159 pKa = 5.38IHH161 pKa = 5.75WFWEE165 pKa = 4.56SIGGVGKK172 pKa = 10.81SSFAKK177 pKa = 9.99YY178 pKa = 10.21LCSVHH183 pKa = 5.6NAIYY187 pKa = 9.96IDD189 pKa = 3.82EE190 pKa = 4.67GKK192 pKa = 10.64KK193 pKa = 10.09SDD195 pKa = 5.45LINIVYY201 pKa = 9.59NIPNITCRR209 pKa = 11.84SIIVIDD215 pKa = 3.71VPRR218 pKa = 11.84DD219 pKa = 3.34NGNTVSYY226 pKa = 10.62KK227 pKa = 10.61AIEE230 pKa = 4.02QIKK233 pKa = 10.84NGMICNTKK241 pKa = 10.19YY242 pKa = 8.43EE243 pKa = 4.14TGMRR247 pKa = 11.84LFNPPHH253 pKa = 7.55IIIFANMPPEE263 pKa = 4.0EE264 pKa = 4.86HH265 pKa = 7.11KK266 pKa = 11.12LSADD270 pKa = 2.89RR271 pKa = 11.84WKK273 pKa = 9.23ITEE276 pKa = 3.83ISSPP280 pKa = 3.5

Molecular weight: 31.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9N6|A0A126G9N6_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-19 OX=1685745 PE=4 SV=1
MM1 pKa = 8.02PLRR4 pKa = 11.84KK5 pKa = 9.37KK6 pKa = 10.14VFKK9 pKa = 10.2KK10 pKa = 10.43KK11 pKa = 10.31RR12 pKa = 11.84MPKK15 pKa = 9.68RR16 pKa = 11.84KK17 pKa = 8.75PRR19 pKa = 11.84IPKK22 pKa = 9.79GIKK25 pKa = 9.65SYY27 pKa = 10.38ISKK30 pKa = 9.15TIRR33 pKa = 11.84SNEE36 pKa = 3.94EE37 pKa = 3.57TKK39 pKa = 10.07LTSVTAGFTGYY50 pKa = 10.25NSSISSVGDD59 pKa = 3.36YY60 pKa = 7.3VTCLPPVPQGTGQSQRR76 pKa = 11.84IGQAIRR82 pKa = 11.84PIKK85 pKa = 10.22LVIRR89 pKa = 11.84GYY91 pKa = 10.72VIYY94 pKa = 10.87SADD97 pKa = 3.48SQLNARR103 pKa = 11.84MIGGRR108 pKa = 11.84MFCFSDD114 pKa = 3.43KK115 pKa = 10.43TVSNYY120 pKa = 9.62GVATSAGANYY130 pKa = 10.56NLLDD134 pKa = 3.9VGGTSQTFDD143 pKa = 3.31GTVARR148 pKa = 11.84YY149 pKa = 7.49EE150 pKa = 4.15YY151 pKa = 10.18PHH153 pKa = 7.12NNDD156 pKa = 2.52QFKK159 pKa = 10.58FYY161 pKa = 10.12IDD163 pKa = 3.03KK164 pKa = 10.19KK165 pKa = 10.0FRR167 pKa = 11.84MMKK170 pKa = 8.9PWGYY174 pKa = 8.65TNVGSTSTQAIVSMDD189 pKa = 2.72KK190 pKa = 11.07SMYY193 pKa = 10.17RR194 pKa = 11.84PFTITLTQKK203 pKa = 10.18QLPAILKK210 pKa = 9.76YY211 pKa = 10.44DD212 pKa = 3.27QQLNVNFPTNFAPYY226 pKa = 9.66VALGYY231 pKa = 10.29CDD233 pKa = 4.61LLSYY237 pKa = 10.19PADD240 pKa = 3.49TVVTQLNMTFTSTLYY255 pKa = 10.86FKK257 pKa = 10.85DD258 pKa = 3.3AA259 pKa = 4.05

Molecular weight: 29.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

539

259

280

269.5

30.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.453 ± 0.654

1.855 ± 0.478

4.267 ± 0.251

3.711 ± 1.751

4.082 ± 0.643

7.236 ± 0.196

2.412 ± 1.39

8.349 ± 1.755

8.349 ± 0.165

6.308 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.602

5.937 ± 0.63

5.195 ± 0.409

3.711 ± 0.898

3.896 ± 0.771

7.978 ± 0.089

6.865 ± 1.648

5.566 ± 0.685

1.113 ± 0.499

6.122 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski