Apis mellifera associated microvirus 20

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTI4|A0A3S8UTI4_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 20 OX=2494748 PE=4 SV=1
MM1 pKa = 7.63LLHH4 pKa = 7.14AFTVFDD10 pKa = 4.58SKK12 pKa = 11.28SKK14 pKa = 10.64SYY16 pKa = 8.21NTPWFARR23 pKa = 11.84SKK25 pKa = 10.95PEE27 pKa = 3.55AEE29 pKa = 4.0RR30 pKa = 11.84NFVQLVKK37 pKa = 10.74DD38 pKa = 3.98EE39 pKa = 4.54KK40 pKa = 11.61SMVNQFPEE48 pKa = 4.65DD49 pKa = 3.35FDD51 pKa = 4.24LFYY54 pKa = 10.78IGTYY58 pKa = 10.58DD59 pKa = 4.57DD60 pKa = 3.64NTAKK64 pKa = 10.3FDD66 pKa = 4.05LLEE69 pKa = 4.52TPEE72 pKa = 4.51HH73 pKa = 5.84MFKK76 pKa = 11.01ALTLKK81 pKa = 10.46NQSS84 pKa = 3.08

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTH3|A0A3S8UTH3_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 20 OX=2494748 PE=3 SV=1
MM1 pKa = 7.86EE2 pKa = 4.75STLLTPSTHH11 pKa = 6.34ALCVVEE17 pKa = 4.57SASNKK22 pKa = 9.83KK23 pKa = 9.77KK24 pKa = 10.29GHH26 pKa = 4.89TMRR29 pKa = 11.84CTSPRR34 pKa = 11.84TVGYY38 pKa = 9.86QADD41 pKa = 4.27GSSLSWSPKK50 pKa = 8.54TFSKK54 pKa = 10.61EE55 pKa = 3.62YY56 pKa = 9.86ATFQLPCGKK65 pKa = 9.87CLSCRR70 pKa = 11.84LEE72 pKa = 4.05YY73 pKa = 10.58ARR75 pKa = 11.84QWAVRR80 pKa = 11.84CVHH83 pKa = 6.75EE84 pKa = 5.05SMCHH88 pKa = 5.12QEE90 pKa = 3.8NCFITLTYY98 pKa = 10.58SDD100 pKa = 5.2EE101 pKa = 4.11NLKK104 pKa = 10.41SDD106 pKa = 3.29KK107 pKa = 10.69LQYY110 pKa = 10.51RR111 pKa = 11.84DD112 pKa = 3.54FQLFMKK118 pKa = 10.18RR119 pKa = 11.84LRR121 pKa = 11.84KK122 pKa = 9.65AFPNKK127 pKa = 10.07EE128 pKa = 3.61IGFNAVGEE136 pKa = 4.43YY137 pKa = 10.7GDD139 pKa = 3.71KK140 pKa = 10.34TKK142 pKa = 10.65RR143 pKa = 11.84PHH145 pKa = 4.14WHH147 pKa = 6.91AIIFNWQPSDD157 pKa = 3.44SQYY160 pKa = 10.71RR161 pKa = 11.84RR162 pKa = 11.84SNEE165 pKa = 3.44RR166 pKa = 11.84GDD168 pKa = 4.52RR169 pKa = 11.84IFSSKK174 pKa = 9.53TLDD177 pKa = 3.64KK178 pKa = 11.07LWGLGITEE186 pKa = 4.58FGSVTFHH193 pKa = 6.15SAGYY197 pKa = 8.55VSRR200 pKa = 11.84YY201 pKa = 7.14NAKK204 pKa = 10.33KK205 pKa = 10.24LIHH208 pKa = 6.69GKK210 pKa = 8.84DD211 pKa = 3.55QEE213 pKa = 4.64HH214 pKa = 7.3DD215 pKa = 3.42LHH217 pKa = 6.78PISKK221 pKa = 9.98KK222 pKa = 9.09SNKK225 pKa = 9.02HH226 pKa = 5.82AIGKK230 pKa = 9.0KK231 pKa = 7.81WLEE234 pKa = 4.58KK235 pKa = 9.76YY236 pKa = 8.98WQDD239 pKa = 3.28AFNHH243 pKa = 6.06GEE245 pKa = 4.14IILADD250 pKa = 3.94GTSTAIPRR258 pKa = 11.84YY259 pKa = 7.05YY260 pKa = 10.54EE261 pKa = 3.61KK262 pKa = 10.07WLKK265 pKa = 10.14EE266 pKa = 4.18NQPEE270 pKa = 4.02AYY272 pKa = 9.59LRR274 pKa = 11.84YY275 pKa = 8.68VTQTKK280 pKa = 9.32IRR282 pKa = 11.84KK283 pKa = 8.91IEE285 pKa = 3.99AAQEE289 pKa = 4.03KK290 pKa = 9.29AAKK293 pKa = 8.97EE294 pKa = 3.85ATRR297 pKa = 11.84AEE299 pKa = 4.26LANHH303 pKa = 6.29KK304 pKa = 10.3RR305 pKa = 11.84RR306 pKa = 11.84TASLLDD312 pKa = 4.41GYY314 pKa = 11.1HH315 pKa = 6.82RR316 pKa = 11.84GHH318 pKa = 6.7EE319 pKa = 3.91ISRR322 pKa = 11.84NEE324 pKa = 3.78INKK327 pKa = 9.87KK328 pKa = 8.45IQQARR333 pKa = 11.84FKK335 pKa = 10.73QLQDD339 pKa = 3.34NLKK342 pKa = 10.42LL343 pKa = 3.74

Molecular weight: 39.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1320

84

520

264.0

29.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.561 ± 1.729

0.758 ± 0.49

5.0 ± 0.809

6.061 ± 0.842

4.924 ± 1.205

5.985 ± 0.965

2.576 ± 0.653

5.455 ± 0.629

6.97 ± 1.963

7.424 ± 0.804

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.333 ± 1.045

5.833 ± 0.79

4.848 ± 0.616

5.0 ± 0.818

5.455 ± 0.856

6.439 ± 0.524

6.667 ± 0.888

4.47 ± 0.579

1.288 ± 0.474

2.955 ± 0.667
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski