Salmonella phage P22 (Bacteriophage P22)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P14110|RPC3_BPP22 Regulatory protein C3 OS=Salmonella phage P22 OX=10754 GN=C3 PE=3 SV=1
MM1 pKa = 7.81KK2 pKa = 10.33LRR4 pKa = 11.84VWHH7 pKa = 6.62IPQVPMKK14 pKa = 9.66PFIVEE19 pKa = 3.89VGSVEE24 pKa = 3.92EE25 pKa = 4.47GVRR28 pKa = 11.84MMDD31 pKa = 4.09ALADD35 pKa = 3.83YY36 pKa = 10.94DD37 pKa = 4.43AFQYY41 pKa = 11.41DD42 pKa = 4.1NNIKK46 pKa = 10.06PDD48 pKa = 3.47YY49 pKa = 10.48CNANGLQMFDD59 pKa = 4.55EE60 pKa = 4.74SLTDD64 pKa = 3.69QDD66 pKa = 5.0LEE68 pKa = 5.09DD69 pKa = 4.43MEE71 pKa = 6.96LDD73 pKa = 4.23DD74 pKa = 6.45RR75 pKa = 11.84WIDD78 pKa = 3.34WYY80 pKa = 10.77SEE82 pKa = 4.16CQCYY86 pKa = 10.36DD87 pKa = 3.26DD88 pKa = 3.95PRR90 pKa = 11.84EE91 pKa = 3.93YY92 pKa = 11.13LEE94 pKa = 4.09SLKK97 pKa = 11.11EE98 pKa = 3.88EE99 pKa = 4.04TTAAA103 pKa = 3.74

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q38665|NINX_BPP22 Putative protein ninX OS=Salmonella phage P22 OX=10754 GN=ninX PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84SITDD10 pKa = 4.1IICEE14 pKa = 3.96NCKK17 pKa = 10.21YY18 pKa = 10.77LPTKK22 pKa = 10.4RR23 pKa = 11.84SRR25 pKa = 11.84NKK27 pKa = 9.9RR28 pKa = 11.84KK29 pKa = 9.44PIPKK33 pKa = 9.7EE34 pKa = 3.78SDD36 pKa = 3.19VKK38 pKa = 9.6TFNYY42 pKa = 7.51TAHH45 pKa = 6.73LWDD48 pKa = 3.94IRR50 pKa = 11.84WLRR53 pKa = 11.84HH54 pKa = 5.19RR55 pKa = 11.84ARR57 pKa = 11.84KK58 pKa = 8.96

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

1

73

13073

25

725

179.1

20.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.462 ± 0.56

1.3 ± 0.172

5.913 ± 0.279

6.563 ± 0.378

3.526 ± 0.259

6.869 ± 0.411

1.645 ± 0.158

6.563 ± 0.356

6.349 ± 0.313

7.932 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.014 ± 0.149

4.743 ± 0.252

3.97 ± 0.207

4.406 ± 0.347

5.798 ± 0.264

6.081 ± 0.23

5.133 ± 0.254

5.829 ± 0.266

1.438 ± 0.139

3.465 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski