Hoeflea olei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Hoeflea

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C1YU20|A0A1C1YU20_9RHIZ Glyoxalase OS=Hoeflea olei OX=1480615 GN=AWJ14_06975 PE=4 SV=1
MM1 pKa = 6.93EE2 pKa = 6.04HH3 pKa = 6.53IAAFMILVACNGGYY17 pKa = 10.11DD18 pKa = 3.57SCSEE22 pKa = 4.01QPAPAVAYY30 pKa = 7.3EE31 pKa = 4.17TVQQCEE37 pKa = 4.47AEE39 pKa = 4.27LSPAIRR45 pKa = 11.84MMAAKK50 pKa = 9.61QDD52 pKa = 3.68HH53 pKa = 6.79ALGKK57 pKa = 10.32CVEE60 pKa = 4.44IDD62 pKa = 3.37PALLYY67 pKa = 10.72EE68 pKa = 4.54DD69 pKa = 5.72AEE71 pKa = 4.66IVWDD75 pKa = 3.85VSKK78 pKa = 11.23DD79 pKa = 3.59GEE81 pKa = 4.22IEE83 pKa = 4.17VAIEE87 pKa = 5.06LIDD90 pKa = 4.43PEE92 pKa = 5.07INLQTLAQSDD102 pKa = 4.15SNRR105 pKa = 11.84DD106 pKa = 2.97EE107 pKa = 4.07TRR109 pKa = 11.84QMNN112 pKa = 3.63

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C1YQB1|A0A1C1YQB1_9RHIZ Methyltransferase OS=Hoeflea olei OX=1480615 GN=AWJ14_06200 PE=4 SV=1
MM1 pKa = 7.13ARR3 pKa = 11.84KK4 pKa = 9.18RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84WRR9 pKa = 11.84SFQASLDD16 pKa = 3.42PRR18 pKa = 11.84RR19 pKa = 11.84LVFIDD24 pKa = 3.49EE25 pKa = 4.37TWIKK29 pKa = 10.39TNMAPLRR36 pKa = 11.84GWEE39 pKa = 4.1QKK41 pKa = 10.02GKK43 pKa = 9.95RR44 pKa = 11.84LRR46 pKa = 11.84GFAPHH51 pKa = 5.85GHH53 pKa = 6.02WRR55 pKa = 11.84TLTFLGALRR64 pKa = 11.84CDD66 pKa = 3.95RR67 pKa = 11.84LTAPCVFDD75 pKa = 4.65GPINAQCFRR84 pKa = 11.84AYY86 pKa = 10.48VEE88 pKa = 4.13QQLVPVLRR96 pKa = 11.84PGDD99 pKa = 3.82IVIMDD104 pKa = 3.97NLGSHH109 pKa = 6.76KK110 pKa = 10.6SVAIRR115 pKa = 11.84QAITAAGARR124 pKa = 11.84LWFLPPYY131 pKa = 10.6SPDD134 pKa = 3.5LNPIEE139 pKa = 4.12QAFAKK144 pKa = 9.36IKK146 pKa = 9.94HH147 pKa = 5.52WMRR150 pKa = 11.84QAQKK154 pKa = 9.8RR155 pKa = 11.84TIEE158 pKa = 4.19DD159 pKa = 3.14TWRR162 pKa = 11.84HH163 pKa = 5.2IGHH166 pKa = 8.28LITSIQPTEE175 pKa = 3.82CRR177 pKa = 11.84NYY179 pKa = 10.8LEE181 pKa = 4.29NVGYY185 pKa = 11.01ASVKK189 pKa = 8.01TT190 pKa = 3.87

Molecular weight: 22.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4182

0

4182

1338745

41

3908

320.1

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.904 ± 0.053

0.826 ± 0.011

5.854 ± 0.033

5.78 ± 0.041

3.801 ± 0.02

8.819 ± 0.051

2.024 ± 0.021

5.311 ± 0.024

3.12 ± 0.034

9.943 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.021

2.531 ± 0.022

5.039 ± 0.029

2.841 ± 0.02

6.949 ± 0.045

5.515 ± 0.034

5.324 ± 0.041

7.323 ± 0.029

1.275 ± 0.017

2.189 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski