Plantactinospora sp. KBS50

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Plantactinospora; unclassified Plantactinospora

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A248YWG9|A0A248YWG9_9ACTN Uncharacterized protein OS=Plantactinospora sp. KBS50 OX=2024580 GN=CIK06_15420 PE=4 SV=1
MM1 pKa = 7.13VGYY4 pKa = 10.26NVTIMNEE11 pKa = 3.98SALDD15 pKa = 3.58GMNAILFQEE24 pKa = 4.66MPSLPSDD31 pKa = 3.48AVTLAWMSKK40 pKa = 7.68MCHH43 pKa = 6.42PSTSIEE49 pKa = 4.17YY50 pKa = 9.29QWSIDD55 pKa = 3.51YY56 pKa = 11.06SFVWGQVGTLVPGTQFVAGQILAADD81 pKa = 3.8LTEE84 pKa = 4.47NNLVTLSYY92 pKa = 11.24VDD94 pKa = 3.68GGFEE98 pKa = 4.17FGPLGSSGKK107 pKa = 10.31DD108 pKa = 3.04GSLTIKK114 pKa = 10.47EE115 pKa = 4.6DD116 pKa = 3.57GSVPGPGNADD126 pKa = 3.23QGSVGIGMSGAGTFVVPTLTNAGVEE151 pKa = 4.27FDD153 pKa = 4.52PKK155 pKa = 8.02PTYY158 pKa = 9.07WVAFGSFASSEE169 pKa = 4.11VMDD172 pKa = 3.93ISEE175 pKa = 4.82LTTPAQVTYY184 pKa = 10.59PDD186 pKa = 4.23GLTSAKK192 pKa = 10.17AVYY195 pKa = 9.72DD196 pKa = 3.76GKK198 pKa = 10.92EE199 pKa = 3.46WTITYY204 pKa = 10.66GNN206 pKa = 3.72

Molecular weight:
21.73 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A248YLN4|A0A248YLN4_9ACTN LLM class flavin-dependent oxidoreductase OS=Plantactinospora sp. KBS50 OX=2024580 GN=CIK06_11060 PE=4 SV=1
MM1 pKa = 6.74VGLRR5 pKa = 11.84VGHH8 pKa = 6.87LRR10 pKa = 11.84LTRR13 pKa = 11.84PGRR16 pKa = 11.84GRR18 pKa = 11.84SAHH21 pKa = 6.65PGRR24 pKa = 11.84TVRR27 pKa = 11.84ATTGLGARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84PVTRR43 pKa = 11.84PAGAAAIGPRR53 pKa = 11.84PVVVAAA59 pKa = 4.74

Molecular weight:
6.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5179

0

5179

1768842

30

4222

341.5

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.263 ± 0.051

0.791 ± 0.01

6.087 ± 0.03

4.842 ± 0.035

2.62 ± 0.019

9.73 ± 0.031

2.083 ± 0.016

3.227 ± 0.024

1.379 ± 0.021

10.539 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.574 ± 0.013

1.735 ± 0.02

6.595 ± 0.04

2.673 ± 0.017

8.815 ± 0.042

4.756 ± 0.027

5.916 ± 0.032

8.734 ± 0.029

1.551 ± 0.016

2.089 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski