Bacillus phage vB_BsuM-Goe3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Grisebachstrassevirus; Bacillus virus vBBsuMGoe3

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A217EQY5|A0A217EQY5_9CAUD Uncharacterized protein OS=Bacillus phage vB_BsuM-Goe3 OX=1933063 GN=Goe3_c01400 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.18KK3 pKa = 10.21VKK5 pKa = 10.32AYY7 pKa = 10.86LLDD10 pKa = 3.78NMEE13 pKa = 4.14EE14 pKa = 4.05LKK16 pKa = 11.15DD17 pKa = 3.65VVRR20 pKa = 11.84EE21 pKa = 4.0INSWNGDD28 pKa = 3.83LGHH31 pKa = 7.62LDD33 pKa = 4.04YY34 pKa = 11.4EE35 pKa = 5.15DD36 pKa = 4.12NDD38 pKa = 3.24EE39 pKa = 5.63DD40 pKa = 4.38FFNTYY45 pKa = 10.33FEE47 pKa = 4.65GRR49 pKa = 11.84PHH51 pKa = 5.75EE52 pKa = 4.35AVRR55 pKa = 11.84AVCYY59 pKa = 10.56GDD61 pKa = 3.54YY62 pKa = 10.91GYY64 pKa = 10.29MDD66 pKa = 5.88DD67 pKa = 4.01YY68 pKa = 11.89VKK70 pKa = 10.91FDD72 pKa = 3.77GYY74 pKa = 11.44DD75 pKa = 3.35NLVSISEE82 pKa = 4.11YY83 pKa = 10.96DD84 pKa = 3.4LEE86 pKa = 4.69EE87 pKa = 3.98EE88 pKa = 3.92LKK90 pKa = 11.09EE91 pKa = 4.15NIEE94 pKa = 4.55EE95 pKa = 4.59IIQSLDD101 pKa = 3.41RR102 pKa = 11.84NHH104 pKa = 6.99TNLCLSDD111 pKa = 4.18EE112 pKa = 4.23LTALINEE119 pKa = 4.13EE120 pKa = 4.34DD121 pKa = 4.05EE122 pKa = 4.44EE123 pKa = 4.61EE124 pKa = 4.2EE125 pKa = 4.19

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z1DF10|A0A1Z1DF10_9CAUD Tail lysin OS=Bacillus phage vB_BsuM-Goe3 OX=1933063 GN=Goe3_c08500 PE=3 SV=1
MM1 pKa = 7.49PRR3 pKa = 11.84KK4 pKa = 9.96KK5 pKa = 10.21SVRR8 pKa = 11.84PKK10 pKa = 10.46LFKK13 pKa = 11.01SNSATKK19 pKa = 9.51STMKK23 pKa = 10.68RR24 pKa = 11.84LGDD27 pKa = 3.54TLVQKK32 pKa = 9.45TLDD35 pKa = 3.5AGMDD39 pKa = 3.68AARR42 pKa = 11.84DD43 pKa = 3.84ALPSDD48 pKa = 3.81TNVVRR53 pKa = 11.84KK54 pKa = 9.07PKK56 pKa = 10.01YY57 pKa = 9.58LQVTEE62 pKa = 3.98KK63 pKa = 10.69RR64 pKa = 11.84MNKK67 pKa = 10.15LGIIDD72 pKa = 4.31LKK74 pKa = 11.04PYY76 pKa = 9.97FKK78 pKa = 10.69KK79 pKa = 10.71SPKK82 pKa = 8.81TKK84 pKa = 8.87RR85 pKa = 11.84TKK87 pKa = 10.41SGGWYY92 pKa = 8.21LTVPISIKK100 pKa = 10.46KK101 pKa = 9.49KK102 pKa = 9.11DD103 pKa = 3.56MSRR106 pKa = 11.84RR107 pKa = 11.84MYY109 pKa = 10.67DD110 pKa = 2.97QLRR113 pKa = 11.84LEE115 pKa = 5.06RR116 pKa = 11.84IAPSTQKK123 pKa = 9.5TVISDD128 pKa = 3.37YY129 pKa = 10.98LYY131 pKa = 10.55DD132 pKa = 3.6RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84TSEE138 pKa = 4.03SSALNYY144 pKa = 9.67KK145 pKa = 9.94PKK147 pKa = 10.71SNNVTKK153 pKa = 10.36TRR155 pKa = 11.84TGKK158 pKa = 9.49NRR160 pKa = 11.84HH161 pKa = 6.09SYY163 pKa = 8.22VAYY166 pKa = 8.91RR167 pKa = 11.84TVTDD171 pKa = 4.35KK172 pKa = 11.48SPASSWVINRR182 pKa = 11.84GKK184 pKa = 10.51VNKK187 pKa = 9.44DD188 pKa = 3.06DD189 pKa = 3.86HH190 pKa = 7.22SKK192 pKa = 9.39TFVKK196 pKa = 10.71NVDD199 pKa = 3.82RR200 pKa = 11.84LMKK203 pKa = 9.95WKK205 pKa = 9.36MKK207 pKa = 10.66NGWSS211 pKa = 3.45

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

44817

35

1274

189.9

21.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.241 ± 0.197

0.794 ± 0.069

6.663 ± 0.146

8.191 ± 0.27

3.758 ± 0.102

6.542 ± 0.245

1.789 ± 0.096

6.176 ± 0.135

7.448 ± 0.203

8.421 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.092

4.989 ± 0.138

3.18 ± 0.132

3.59 ± 0.144

4.675 ± 0.136

6.558 ± 0.214

6.047 ± 0.205

7.147 ± 0.176

1.006 ± 0.069

4.228 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski