Erwinia phage vB_EamM_Alexandra

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Alexandravirus; Erwinia virus Alexandra

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QFP7|A0A2Z4QFP7_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Alexandra OX=2201424 GN=Alexandra_290 PE=4 SV=1
MM1 pKa = 7.52SEE3 pKa = 4.7PYY5 pKa = 10.16SKK7 pKa = 11.2CEE9 pKa = 4.21DD10 pKa = 3.3CRR12 pKa = 11.84TPLKK16 pKa = 10.34EE17 pKa = 3.94DD18 pKa = 3.21EE19 pKa = 4.81VYY21 pKa = 11.28GEE23 pKa = 4.34TVDD26 pKa = 5.19LCEE29 pKa = 4.24NCCADD34 pKa = 4.92RR35 pKa = 11.84EE36 pKa = 4.45DD37 pKa = 4.49EE38 pKa = 4.65GEE40 pKa = 4.28STT42 pKa = 4.56

Molecular weight: 4.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QF75|A0A2Z4QF75_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Alexandra OX=2201424 GN=Alexandra_323 PE=4 SV=1
MM1 pKa = 8.07AYY3 pKa = 10.05RR4 pKa = 11.84PRR6 pKa = 11.84GGIAARR12 pKa = 11.84TQGGRR17 pKa = 11.84GRR19 pKa = 11.84SLSTSQRR26 pKa = 11.84LAGVTGFEE34 pKa = 4.07RR35 pKa = 11.84ARR37 pKa = 11.84AGTHH41 pKa = 4.59VRR43 pKa = 11.84MDD45 pKa = 3.33RR46 pKa = 11.84ADD48 pKa = 3.25WTKK51 pKa = 11.29LKK53 pKa = 10.4MSIIDD58 pKa = 3.37EE59 pKa = 4.1RR60 pKa = 11.84GYY62 pKa = 10.17YY63 pKa = 9.48CEE65 pKa = 4.79RR66 pKa = 11.84CGKK69 pKa = 7.09PTRR72 pKa = 11.84EE73 pKa = 4.42LILNHH78 pKa = 6.9KK79 pKa = 8.8IAHH82 pKa = 6.34ANGGSNMKK90 pKa = 10.41HH91 pKa = 5.72NLEE94 pKa = 4.27LLCYY98 pKa = 9.61TCDD101 pKa = 3.35NNQIGTANRR110 pKa = 11.84RR111 pKa = 11.84GSRR114 pKa = 11.84LLHH117 pKa = 6.57GGRR120 pKa = 11.84KK121 pKa = 8.82

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

345

0

345

85358

35

1948

247.4

27.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.476 ± 0.167

1.183 ± 0.062

6.784 ± 0.161

6.024 ± 0.176

3.897 ± 0.087

6.47 ± 0.141

2.069 ± 0.085

5.34 ± 0.081

6.146 ± 0.234

8.285 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.912 ± 0.083

4.556 ± 0.092

4.38 ± 0.086

3.796 ± 0.09

5.386 ± 0.109

6.274 ± 0.129

6.204 ± 0.177

7.235 ± 0.135

1.152 ± 0.046

3.43 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski