Butyrivibrio fibrisolvens 16/4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; Butyrivibrio fibrisolvens

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4IYZ5|D4IYZ5_BUTFI Serine/threonine protein phosphatase OS=Butyrivibrio fibrisolvens 16/4 OX=657324 GN=CIY_00990 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.43KK3 pKa = 10.23KK4 pKa = 10.39LLSVLLTGAMAASMFVGCGTSTEE27 pKa = 4.45TTDD30 pKa = 3.4NAAPAAEE37 pKa = 4.54GEE39 pKa = 4.47ATEE42 pKa = 4.44STTTAGGTKK51 pKa = 10.07VGVAMPTKK59 pKa = 10.47DD60 pKa = 3.5LQRR63 pKa = 11.84WNQDD67 pKa = 2.73GANMQKK73 pKa = 9.92EE74 pKa = 4.31LEE76 pKa = 4.18AAGYY80 pKa = 9.76EE81 pKa = 4.1VDD83 pKa = 5.01LQYY86 pKa = 11.69ASNDD90 pKa = 3.26TQTQVSQIEE99 pKa = 3.94NMINSGCNVLVVAAIEE115 pKa = 4.18ASSLGEE121 pKa = 4.21AMDD124 pKa = 3.85MAASANIPVIAYY136 pKa = 8.53DD137 pKa = 3.85RR138 pKa = 11.84LIMNTDD144 pKa = 2.95AVSYY148 pKa = 10.6YY149 pKa = 10.63ATFDD153 pKa = 3.26NYY155 pKa = 11.18KK156 pKa = 9.84VGTVQGQYY164 pKa = 10.43IIDD167 pKa = 3.77QLDD170 pKa = 3.59LEE172 pKa = 4.61NTDD175 pKa = 3.16KK176 pKa = 10.97TFTMEE181 pKa = 3.89ITAGDD186 pKa = 4.11PGDD189 pKa = 3.74NNAGFFYY196 pKa = 10.62NGAMDD201 pKa = 4.66LLKK204 pKa = 10.47PYY206 pKa = 9.74IEE208 pKa = 5.09KK209 pKa = 9.2GTIVVKK215 pKa = 10.64SGQTEE220 pKa = 4.19FQDD223 pKa = 3.49VATAAWATEE232 pKa = 4.23TAQSRR237 pKa = 11.84MEE239 pKa = 4.88NILSSYY245 pKa = 9.5YY246 pKa = 10.97ADD248 pKa = 3.83GTDD251 pKa = 6.16LDD253 pKa = 4.18ICLCSNDD260 pKa = 3.31STALGVEE267 pKa = 4.04NALAANYY274 pKa = 8.95NGKK277 pKa = 9.89YY278 pKa = 10.07PIVTGQDD285 pKa = 2.95CDD287 pKa = 3.59IEE289 pKa = 4.41NTKK292 pKa = 11.06NMIAGKK298 pKa = 10.19QSMSVFKK305 pKa = 9.92DD306 pKa = 3.03TRR308 pKa = 11.84TLASQVVKK316 pKa = 10.31MVGQILNGEE325 pKa = 4.21EE326 pKa = 3.89VDD328 pKa = 4.3VNDD331 pKa = 3.5TTTYY335 pKa = 11.16DD336 pKa = 3.56NNNGVVPSFLCDD348 pKa = 3.21PVFADD353 pKa = 3.2VNNYY357 pKa = 9.93KK358 pKa = 10.5EE359 pKa = 3.9ILIDD363 pKa = 3.33SGYY366 pKa = 8.45YY367 pKa = 9.62TEE369 pKa = 6.04DD370 pKa = 3.15QLQQ373 pKa = 3.2

Molecular weight: 40.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4IYT5|D4IYT5_BUTFI Xylose-binding protein OS=Butyrivibrio fibrisolvens 16/4 OX=657324 GN=CIY_00260 PE=4 SV=1
MM1 pKa = 7.47VGVNGLGNMLNRR13 pKa = 11.84YY14 pKa = 6.38GTTVQKK20 pKa = 10.66LKK22 pKa = 10.51RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84LQIQRR31 pKa = 11.84RR32 pKa = 11.84LQRR35 pKa = 11.84LLKK38 pKa = 10.42RR39 pKa = 11.84RR40 pKa = 3.66

Molecular weight: 4.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2904

0

2904

797346

15

2445

274.6

30.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.494 ± 0.049

1.383 ± 0.021

6.386 ± 0.045

7.367 ± 0.053

4.211 ± 0.038

6.9 ± 0.044

1.611 ± 0.022

7.764 ± 0.049

6.97 ± 0.041

8.373 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.025 ± 0.029

5.055 ± 0.035

3.083 ± 0.029

2.817 ± 0.024

3.64 ± 0.033

6.128 ± 0.083

5.546 ± 0.047

7.083 ± 0.04

0.874 ± 0.018

4.289 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski