Echinicola vietnamensis (strain DSM 17526 / LMG 23754 / KMM 6221)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Echinicola; Echinicola vietnamensis

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0FUQ2|L0FUQ2_ECHVK Uncharacterized protein OS=Echinicola vietnamensis (strain DSM 17526 / LMG 23754 / KMM 6221) OX=926556 GN=Echvi_1350 PE=4 SV=1
MM1 pKa = 7.48HH2 pKa = 7.69KK3 pKa = 10.05ISYY6 pKa = 10.1ISILVVCAILVGYY19 pKa = 8.41VPKK22 pKa = 10.79DD23 pKa = 3.47VVSNSGVKK31 pKa = 10.1VEE33 pKa = 4.52SVRR36 pKa = 11.84KK37 pKa = 9.85NINSDD42 pKa = 2.66TDD44 pKa = 3.62YY45 pKa = 11.86DD46 pKa = 4.05EE47 pKa = 6.96DD48 pKa = 5.34GVLNDD53 pKa = 5.25NDD55 pKa = 5.49LDD57 pKa = 4.43DD58 pKa = 6.26DD59 pKa = 4.41NDD61 pKa = 4.89GILDD65 pKa = 3.44IDD67 pKa = 3.85EE68 pKa = 5.61GFVCTTNSQKK78 pKa = 9.51TVLKK82 pKa = 10.39AISNNIPAEE91 pKa = 4.18GTSYY95 pKa = 11.14CGGLKK100 pKa = 10.28NSYY103 pKa = 7.4THH105 pKa = 7.2FSDD108 pKa = 6.02GYY110 pKa = 11.1LDD112 pKa = 3.73TQNDD116 pKa = 3.07ITGTYY121 pKa = 7.87TLNYY125 pKa = 9.1SYY127 pKa = 11.37SGLDD131 pKa = 3.29VSKK134 pKa = 10.75EE135 pKa = 3.87AIFQHH140 pKa = 6.17RR141 pKa = 11.84LVYY144 pKa = 10.83SNVNGSHH151 pKa = 6.5GVDD154 pKa = 2.97QNTSNVRR161 pKa = 11.84ITSKK165 pKa = 10.35IYY167 pKa = 10.73VNNTLVRR174 pKa = 11.84TASVTGWNYY183 pKa = 10.09HH184 pKa = 4.5SQPRR188 pKa = 11.84EE189 pKa = 3.67KK190 pKa = 10.7DD191 pKa = 2.89NKK193 pKa = 10.02YY194 pKa = 11.09YY195 pKa = 11.05YY196 pKa = 7.28FTPSTASGTVRR207 pKa = 11.84IDD209 pKa = 3.06ITFEE213 pKa = 3.96RR214 pKa = 11.84LNGYY218 pKa = 9.89CDD220 pKa = 3.98VIPEE224 pKa = 4.18VFFGGNLSQTSDD236 pKa = 4.79PICTSIDD243 pKa = 3.37TDD245 pKa = 3.69GDD247 pKa = 4.4GIPDD251 pKa = 3.97HH252 pKa = 7.11QDD254 pKa = 2.93LDD256 pKa = 4.2SDD258 pKa = 4.88GDD260 pKa = 3.95GCPDD264 pKa = 3.36ALEE267 pKa = 4.51GQGGFTAADD276 pKa = 3.81LVSSALAGGNSGANYY291 pKa = 8.97TGVAGSVVSNLGATVGSNGVPTKK314 pKa = 10.41AASGQGRR321 pKa = 11.84GTSRR325 pKa = 11.84EE326 pKa = 3.96IQQVASACIGCSTLAGDD343 pKa = 3.97VTDD346 pKa = 3.92TGGDD350 pKa = 3.53PVGNAMIRR358 pKa = 11.84VYY360 pKa = 11.41ADD362 pKa = 3.61FDD364 pKa = 4.14QNGNISSGDD373 pKa = 3.52EE374 pKa = 3.75LLKK377 pKa = 10.93VVNADD382 pKa = 3.09AQGAYY387 pKa = 10.14SINKK391 pKa = 9.24GYY393 pKa = 10.69YY394 pKa = 8.11ITVKK398 pKa = 10.67DD399 pKa = 4.45DD400 pKa = 3.65FSGDD404 pKa = 3.47VFSGNNNSSGDD415 pKa = 3.59YY416 pKa = 10.27NAWLDD421 pKa = 4.39ASWTQSNSANSGISSNTNGDD441 pKa = 3.73DD442 pKa = 3.73NLEE445 pKa = 3.98SRR447 pKa = 11.84HH448 pKa = 6.6IYY450 pKa = 8.77IHH452 pKa = 6.74NNTQVYY458 pKa = 9.58RR459 pKa = 11.84AVNLSKK465 pKa = 10.91FKK467 pKa = 9.86TANLRR472 pKa = 11.84FDD474 pKa = 4.25YY475 pKa = 9.39DD476 pKa = 3.77TEE478 pKa = 4.59NDD480 pKa = 3.58LDD482 pKa = 3.93ATDD485 pKa = 4.0KK486 pKa = 11.19FHH488 pKa = 8.01VEE490 pKa = 3.84LSKK493 pKa = 11.44DD494 pKa = 3.12GGATYY499 pKa = 7.68TTVYY503 pKa = 9.93TYY505 pKa = 11.24TDD507 pKa = 3.81GNVDD511 pKa = 3.54NQPARR516 pKa = 11.84SQNINLNGYY525 pKa = 9.3AGEE528 pKa = 4.29SNVIIRR534 pKa = 11.84FRR536 pKa = 11.84AEE538 pKa = 3.96SSSGEE543 pKa = 4.37YY544 pKa = 9.61IWLDD548 pKa = 3.01NVTIYY553 pKa = 11.15NAFPTVLLKK562 pKa = 10.24PVYY565 pKa = 9.43NEE567 pKa = 4.58PYY569 pKa = 9.83RR570 pKa = 11.84SSNPATIAVNPSTTTCSGLDD590 pKa = 3.52FTLLDD595 pKa = 4.26ACDD598 pKa = 3.99VDD600 pKa = 4.16PTDD603 pKa = 3.32TDD605 pKa = 3.72GDD607 pKa = 4.72GVCDD611 pKa = 6.08AIDD614 pKa = 4.72IDD616 pKa = 5.27DD617 pKa = 5.49DD618 pKa = 4.87DD619 pKa = 7.17DD620 pKa = 4.5GVLDD624 pKa = 3.58EE625 pKa = 5.13TEE627 pKa = 4.05GHH629 pKa = 6.4FCGEE633 pKa = 4.27LDD635 pKa = 3.51RR636 pKa = 11.84NIIIGYY642 pKa = 9.19LDD644 pKa = 3.55SGVGNDD650 pKa = 4.51GLATDD655 pKa = 4.21MLLNLKK661 pKa = 10.4NYY663 pKa = 10.18GEE665 pKa = 4.45YY666 pKa = 9.54GTYY669 pKa = 10.54DD670 pKa = 3.13KK671 pKa = 11.44VRR673 pKa = 11.84GVVLVPFSSSAEE685 pKa = 3.67ITEE688 pKa = 4.71ANLEE692 pKa = 4.17ANNIDD697 pKa = 3.65VFFAGSSTANSEE709 pKa = 3.98GSSDD713 pKa = 3.79KK714 pKa = 11.12LSSSTNAVLMSWAEE728 pKa = 4.04NNDD731 pKa = 2.96KK732 pKa = 11.27GLFVLQNNAVDD743 pKa = 3.55YY744 pKa = 10.85GYY746 pKa = 8.48TLTNNDD752 pKa = 4.8LPPDD756 pKa = 3.83VPNGEE761 pKa = 4.71LGYY764 pKa = 10.22QVYY767 pKa = 9.63TNGYY771 pKa = 8.24WPVSSITQTGTVKK784 pKa = 8.23MTISSSTRR792 pKa = 11.84SFEE795 pKa = 4.27ALMVDD800 pKa = 3.84SKK802 pKa = 11.61SRR804 pKa = 11.84PTLVKK809 pKa = 10.73DD810 pKa = 3.64NMMKK814 pKa = 10.64LVIFPDD820 pKa = 3.13ATIYY824 pKa = 10.87NDD826 pKa = 3.38EE827 pKa = 4.89ANTVDD832 pKa = 4.21PNKK835 pKa = 10.66NDD837 pKa = 3.37ATKK840 pKa = 10.84VIADD844 pKa = 3.79TWAFVMDD851 pKa = 3.88TFIGDD856 pKa = 3.72GCSEE860 pKa = 5.02LDD862 pKa = 3.67TNGDD866 pKa = 3.54GTPNHH871 pKa = 7.18LDD873 pKa = 3.69LDD875 pKa = 4.25SDD877 pKa = 4.55GDD879 pKa = 3.99GCSDD883 pKa = 3.48AFEE886 pKa = 5.01ANATEE891 pKa = 5.7DD892 pKa = 3.3ITPNFQFDD900 pKa = 3.97VQKK903 pKa = 10.22GTSSDD908 pKa = 3.62TNGDD912 pKa = 3.62GLADD916 pKa = 3.93AVDD919 pKa = 3.97EE920 pKa = 4.43NLNGIPDD927 pKa = 4.14YY928 pKa = 11.42VSAYY932 pKa = 10.65YY933 pKa = 9.89PDD935 pKa = 5.11ALDD938 pKa = 3.75NTTCTDD944 pKa = 3.12SDD946 pKa = 3.74GDD948 pKa = 4.34TIVDD952 pKa = 4.73AVDD955 pKa = 5.13LDD957 pKa = 4.27DD958 pKa = 6.61DD959 pKa = 4.32NDD961 pKa = 4.93GILDD965 pKa = 3.53VDD967 pKa = 4.03EE968 pKa = 5.67GLVCTGANQGEE979 pKa = 4.37FKK981 pKa = 10.32IGYY984 pKa = 9.58VDD986 pKa = 3.43TTVGKK991 pKa = 8.43TGLMEE996 pKa = 4.78NMLTNTDD1003 pKa = 3.15NFGPDD1008 pKa = 3.1GVYY1011 pKa = 10.96DD1012 pKa = 4.6KK1013 pKa = 10.84IPNVTLVPYY1022 pKa = 10.41AINDD1026 pKa = 3.35VSEE1029 pKa = 4.08TRR1031 pKa = 11.84LIEE1034 pKa = 4.1DD1035 pKa = 3.82QIDD1038 pKa = 3.61VFYY1041 pKa = 10.85LGSSTEE1047 pKa = 4.17GDD1049 pKa = 3.91FLNQTGSNSQKK1060 pKa = 10.63LPSTLNSTVRR1070 pKa = 11.84AWGEE1074 pKa = 4.01NNNKK1078 pKa = 9.84SVLVVQNNAIDD1089 pKa = 3.76FGYY1092 pKa = 10.72RR1093 pKa = 11.84LVNNSVNPNTPYY1105 pKa = 10.8DD1106 pKa = 3.72ALGEE1110 pKa = 4.31SVMTNGYY1117 pKa = 8.94WPVSTFDD1124 pKa = 3.27QTGSVQLTASSATEE1138 pKa = 4.01RR1139 pKa = 11.84YY1140 pKa = 9.76DD1141 pKa = 4.73VVMTDD1146 pKa = 3.52KK1147 pKa = 11.15NGKK1150 pKa = 8.35ATLLSSRR1157 pKa = 11.84TSNMVLLPDD1166 pKa = 3.57ATIMKK1171 pKa = 10.25DD1172 pKa = 2.89NSTQSTVSTDD1182 pKa = 2.84ILKK1185 pKa = 10.5VVANIHH1191 pKa = 6.74AYY1193 pKa = 10.41LFDD1196 pKa = 4.06MFLATPQCKK1205 pKa = 10.47GIDD1208 pKa = 3.57TDD1210 pKa = 4.12GDD1212 pKa = 3.87GVANHH1217 pKa = 7.28LDD1219 pKa = 3.86LDD1221 pKa = 4.23SDD1223 pKa = 4.4GDD1225 pKa = 3.87GCPDD1229 pKa = 3.09IKK1231 pKa = 10.86EE1232 pKa = 4.29AGISNYY1238 pKa = 9.7LFKK1241 pKa = 10.92NDD1243 pKa = 3.75QEE1245 pKa = 4.71SLMVSGEE1252 pKa = 4.19VVNLSEE1258 pKa = 4.73TDD1260 pKa = 3.25STQNTTTTIGMSRR1273 pKa = 11.84MNPSNGGDD1281 pKa = 3.04NVGPFNGFHH1290 pKa = 7.78DD1291 pKa = 4.61YY1292 pKa = 11.41LEE1294 pKa = 4.19QTSPGVYY1301 pKa = 10.0EE1302 pKa = 3.86NAPYY1306 pKa = 10.27QLEE1309 pKa = 4.62PYY1311 pKa = 10.4LSNEE1315 pKa = 3.78VNACRR1320 pKa = 11.84IRR1322 pKa = 11.84KK1323 pKa = 9.26IYY1325 pKa = 9.2TNPAMHH1331 pKa = 7.23TPRR1334 pKa = 11.84KK1335 pKa = 9.1AQQ1337 pKa = 3.08

Molecular weight: 143.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0FW70|L0FW70_ECHVK Small-conductance mechanosensitive channel OS=Echinicola vietnamensis (strain DSM 17526 / LMG 23754 / KMM 6221) OX=926556 GN=Echvi_1017 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.5HH16 pKa = 3.99GFRR19 pKa = 11.84EE20 pKa = 4.44RR21 pKa = 11.84MASPNGRR28 pKa = 11.84RR29 pKa = 11.84VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.48GRR39 pKa = 11.84HH40 pKa = 5.05KK41 pKa = 10.93LSVSSEE47 pKa = 3.97KK48 pKa = 9.91TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4509

0

4509

1596854

29

6228

354.1

39.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.066 ± 0.034

0.72 ± 0.012

5.728 ± 0.029

6.852 ± 0.037

4.866 ± 0.028

7.246 ± 0.038

2.094 ± 0.021

6.633 ± 0.032

6.556 ± 0.05

9.429 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.019

4.912 ± 0.033

3.969 ± 0.016

3.759 ± 0.02

4.125 ± 0.027

6.25 ± 0.027

5.359 ± 0.047

6.503 ± 0.031

1.303 ± 0.014

4.063 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski