Poseidonocella pacifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Poseidonocella

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0X4R5|A0A1I0X4R5_9RHOB 50S ribosomal protein L27 OS=Poseidonocella pacifica OX=871651 GN=rpmA PE=3 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84PYY4 pKa = 10.66LALFLPPLLALAPLAQASSIDD25 pKa = 3.82QLKK28 pKa = 8.7LTASDD33 pKa = 3.79AAASDD38 pKa = 3.73TFGWSASISGNTAIVGAYY56 pKa = 10.16ADD58 pKa = 5.13DD59 pKa = 5.41DD60 pKa = 4.85DD61 pKa = 6.44GSGSGSSYY69 pKa = 10.9LFNATTGAQIAKK81 pKa = 8.73LTASDD86 pKa = 3.73AAASDD91 pKa = 3.61RR92 pKa = 11.84FGWSVAISGNTAIVGAIGDD111 pKa = 4.16DD112 pKa = 3.78DD113 pKa = 4.56GGNLSGSSYY122 pKa = 10.9LFNATTGAQIAKK134 pKa = 8.73LTASDD139 pKa = 3.86AAAVDD144 pKa = 3.44QFGYY148 pKa = 10.46SVAISGNTAIVGAYY162 pKa = 10.16ADD164 pKa = 5.26DD165 pKa = 5.74DD166 pKa = 4.88DD167 pKa = 6.87GSDD170 pKa = 3.41SGSAYY175 pKa = 10.59LFNATTGAQIAKK187 pKa = 8.72LTASDD192 pKa = 4.08GAAFDD197 pKa = 4.36NFGQSVAISGNTAIVGAYY215 pKa = 10.24LDD217 pKa = 5.2DD218 pKa = 5.88DD219 pKa = 4.64GGTNSGSAYY228 pKa = 10.42LFNATTGAQIAKK240 pKa = 8.73LTASDD245 pKa = 3.93AAAVDD250 pKa = 3.63YY251 pKa = 10.64FGYY254 pKa = 10.33SVAISGNTAVVGAFADD270 pKa = 4.05DD271 pKa = 4.61DD272 pKa = 4.56GGSTSGSAYY281 pKa = 10.51LFNATTGAQIAKK293 pKa = 8.73LTASDD298 pKa = 3.93AAAVDD303 pKa = 4.02YY304 pKa = 10.55FGSSVAISGNTAIVGAYY321 pKa = 10.05ADD323 pKa = 4.71DD324 pKa = 5.38DD325 pKa = 4.77GGSSSGSAYY334 pKa = 10.61LFNATTGAQITKK346 pKa = 8.95LTASDD351 pKa = 3.91AAAGDD356 pKa = 3.77YY357 pKa = 10.46FGYY360 pKa = 10.26SVAISGTSAIVGAFGDD376 pKa = 4.12DD377 pKa = 3.72DD378 pKa = 4.76GGGGSGSAYY387 pKa = 10.19VFASADD393 pKa = 3.52PSVVPLPAGIWLLGSAAGLLAFGRR417 pKa = 11.84RR418 pKa = 11.84RR419 pKa = 11.84KK420 pKa = 9.9ARR422 pKa = 11.84AAA424 pKa = 3.48

Molecular weight: 41.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0XNM4|A0A1I0XNM4_9RHOB Cell shape-determining protein MreB OS=Poseidonocella pacifica OX=871651 GN=mreB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3383

0

3383

1091612

30

17824

322.7

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.027 ± 0.057

0.875 ± 0.018

6.057 ± 0.046

6.308 ± 0.042

3.757 ± 0.033

8.801 ± 0.066

2.044 ± 0.034

5.279 ± 0.03

2.934 ± 0.042

10.119 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.032

2.503 ± 0.022

5.06 ± 0.054

3.024 ± 0.018

6.96 ± 0.07

5.415 ± 0.06

5.491 ± 0.076

7.199 ± 0.036

1.333 ± 0.02

2.174 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski