Microbacterium phage Shee

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ilzatvirus; unclassified Ilzatvirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514A3F5|A0A514A3F5_9CAUD Uncharacterized protein OS=Microbacterium phage Shee OX=2591072 GN=61 PE=4 SV=1
MM1 pKa = 7.81ACTDD5 pKa = 4.43CDD7 pKa = 4.55DD8 pKa = 4.15KK9 pKa = 11.46QQEE12 pKa = 4.4PFSEE16 pKa = 4.6TEE18 pKa = 3.94GEE20 pKa = 4.5GTVDD24 pKa = 3.4TQEE27 pKa = 4.49TPSEE31 pKa = 4.49VYY33 pKa = 10.59DD34 pKa = 3.71HH35 pKa = 7.36AEE37 pKa = 3.76HH38 pKa = 6.96FGPPEE43 pKa = 3.55AA44 pKa = 5.69

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514A3K8|A0A514A3K8_9CAUD Uncharacterized protein OS=Microbacterium phage Shee OX=2591072 GN=63 PE=4 SV=1
MM1 pKa = 7.21PRR3 pKa = 11.84SMRR6 pKa = 11.84LTSLEE11 pKa = 3.86TRR13 pKa = 11.84IVGVPSAGRR22 pKa = 11.84SGVVPGLEE30 pKa = 4.31LGNGSGVSVGLAPEE44 pKa = 3.94PDD46 pKa = 3.48FRR48 pKa = 11.84QGCSQGHH55 pKa = 5.62SALVPQLKK63 pKa = 8.94EE64 pKa = 3.88NKK66 pKa = 9.26MSGQVLEE73 pKa = 4.9APVSARR79 pKa = 11.84FHH81 pKa = 7.35ASGQQDD87 pKa = 3.08RR88 pKa = 11.84RR89 pKa = 11.84TARR92 pKa = 11.84QDD94 pKa = 3.13GVVCVNRR101 pKa = 11.84LRR103 pKa = 11.84VRR105 pKa = 11.84ATVQNIDD112 pKa = 3.79GQWAEE117 pKa = 4.03VKK119 pKa = 10.69LSVKK123 pKa = 10.38VGEE126 pKa = 4.26GWFPLEE132 pKa = 4.2GGKK135 pKa = 10.12LRR137 pKa = 11.84VARR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84SGQRR146 pKa = 11.84EE147 pKa = 3.92AQEE150 pKa = 4.56LYY152 pKa = 10.49VVSALRR158 pKa = 11.84RR159 pKa = 11.84SLSAHH164 pKa = 5.34GVAVLSLDD172 pKa = 3.34GSEE175 pKa = 3.88QRR177 pKa = 11.84VGDD180 pKa = 3.59GVTDD184 pKa = 3.43YY185 pKa = 10.92RR186 pKa = 11.84YY187 pKa = 7.37WASMGSRR194 pKa = 11.84LTYY197 pKa = 10.53ALEE200 pKa = 3.91VSEE203 pKa = 4.91AGVVSVVNLPP213 pKa = 3.41

Molecular weight: 22.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13207

44

808

209.6

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.752 ± 0.459

0.651 ± 0.095

6.353 ± 0.255

6.58 ± 0.401

3.089 ± 0.19

7.935 ± 0.332

1.878 ± 0.191

5.05 ± 0.369

4.74 ± 0.325

8.526 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.154

3.135 ± 0.153

4.922 ± 0.258

3.99 ± 0.179

5.709 ± 0.358

6.088 ± 0.263

6.398 ± 0.284

7.481 ± 0.274

2.135 ± 0.249

3.082 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski