Microbacterium phage ChickenKing

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Schubertvirus; unclassified Schubertvirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T8S3|A0A5J6T8S3_9CAUD Scaffolding protein OS=Microbacterium phage ChickenKing OX=2652406 GN=5 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.12NNYY5 pKa = 8.3PTFQEE10 pKa = 3.85VRR12 pKa = 11.84NYY14 pKa = 10.72LINDD18 pKa = 3.81NDD20 pKa = 3.48WTAEE24 pKa = 4.04EE25 pKa = 4.27VDD27 pKa = 3.72QLAGYY32 pKa = 9.83AFRR35 pKa = 11.84HH36 pKa = 5.65INQDD40 pKa = 2.44GTTTMTRR47 pKa = 11.84DD48 pKa = 2.52QWTIYY53 pKa = 10.34IMDD56 pKa = 4.08YY57 pKa = 10.82CNEE60 pKa = 4.55HH61 pKa = 6.81IDD63 pKa = 3.8LL64 pKa = 4.85

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T5S0|A0A5J6T5S0_9CAUD Uncharacterized protein OS=Microbacterium phage ChickenKing OX=2652406 GN=50 PE=4 SV=1
MM1 pKa = 7.18TLVLRR6 pKa = 11.84DD7 pKa = 3.77AQRR10 pKa = 11.84VALEE14 pKa = 4.05RR15 pKa = 11.84LTEE18 pKa = 4.35PGRR21 pKa = 11.84HH22 pKa = 5.61FAALWAEE29 pKa = 4.02PRR31 pKa = 11.84SGKK34 pKa = 8.81TGVALSWIQHH44 pKa = 4.36VKK46 pKa = 9.75PRR48 pKa = 11.84VAVVVGPKK56 pKa = 9.68VAEE59 pKa = 4.0ATWRR63 pKa = 11.84SEE65 pKa = 3.64AAKK68 pKa = 9.28WLTMEE73 pKa = 4.08YY74 pKa = 10.5RR75 pKa = 11.84FFPLTTGNEE84 pKa = 4.26YY85 pKa = 10.33PDD87 pKa = 3.4TRR89 pKa = 11.84SFKK92 pKa = 9.77GTTILFVNYY101 pKa = 10.0DD102 pKa = 3.82QFAKK106 pKa = 10.78APWKK110 pKa = 10.55RR111 pKa = 11.84LGPFLKK117 pKa = 10.54KK118 pKa = 9.94MSEE121 pKa = 4.28YY122 pKa = 10.74CQGQGMMLLDD132 pKa = 4.07EE133 pKa = 4.33SHH135 pKa = 7.05IIKK138 pKa = 9.26TPSSVIGRR146 pKa = 11.84NIRR149 pKa = 11.84PLADD153 pKa = 2.73KK154 pKa = 9.38WHH156 pKa = 6.15YY157 pKa = 9.9RR158 pKa = 11.84LLMTGTPVTNPNQIDD173 pKa = 3.49AVYY176 pKa = 9.75GQWTFLDD183 pKa = 4.07PSIRR187 pKa = 11.84DD188 pKa = 3.45HH189 pKa = 6.51WPSARR194 pKa = 11.84DD195 pKa = 3.26FRR197 pKa = 11.84EE198 pKa = 4.06YY199 pKa = 10.4FGEE202 pKa = 3.92WTNVKK207 pKa = 10.04GFPEE211 pKa = 5.13LIKK214 pKa = 10.3PRR216 pKa = 11.84HH217 pKa = 4.5QAEE220 pKa = 4.21LSLYY224 pKa = 8.41LQPNVITMVGPNDD237 pKa = 3.63PVPIRR242 pKa = 11.84KK243 pKa = 8.51VRR245 pKa = 11.84YY246 pKa = 7.52PVPEE250 pKa = 4.54EE251 pKa = 3.47IRR253 pKa = 11.84LQLKK257 pKa = 9.51EE258 pKa = 4.05LFKK261 pKa = 11.07EE262 pKa = 4.74GVVEE266 pKa = 4.29VQGHH270 pKa = 5.97TIAALNPLTRR280 pKa = 11.84LLRR283 pKa = 11.84MRR285 pKa = 11.84SLVAGWAKK293 pKa = 10.88DD294 pKa = 3.67DD295 pKa = 4.09EE296 pKa = 4.92GTSFTVPEE304 pKa = 3.99AARR307 pKa = 11.84ARR309 pKa = 11.84LRR311 pKa = 11.84ALSHH315 pKa = 5.59IVKK318 pKa = 10.04KK319 pKa = 9.61RR320 pKa = 11.84TEE322 pKa = 4.03GKK324 pKa = 10.51VIIACTHH331 pKa = 5.21LWEE334 pKa = 4.19VRR336 pKa = 11.84LVKK339 pKa = 10.41GWMRR343 pKa = 11.84RR344 pKa = 11.84QGIGYY349 pKa = 9.56RR350 pKa = 11.84IITGDD355 pKa = 3.52TKK357 pKa = 11.02EE358 pKa = 4.16KK359 pKa = 10.42NHH361 pKa = 6.58VIEE364 pKa = 4.96DD365 pKa = 3.72FQYY368 pKa = 11.46DD369 pKa = 3.77RR370 pKa = 11.84DD371 pKa = 4.26CKK373 pKa = 10.92VLLVQPRR380 pKa = 11.84TVAMAVDD387 pKa = 3.63ISVANDD393 pKa = 4.38LIWYY397 pKa = 7.42TSDD400 pKa = 3.05FNYY403 pKa = 9.15VTFKK407 pKa = 10.53QASDD411 pKa = 3.99RR412 pKa = 11.84IKK414 pKa = 10.76LSPASPTVWFLCGKK428 pKa = 8.52GTVDD432 pKa = 3.16EE433 pKa = 5.37DD434 pKa = 3.51VWKK437 pKa = 8.91TLQEE441 pKa = 4.24DD442 pKa = 3.71HH443 pKa = 7.37DD444 pKa = 4.54HH445 pKa = 6.72LNKK448 pKa = 9.69TVAKK452 pKa = 9.87IKK454 pKa = 9.35RR455 pKa = 11.84QKK457 pKa = 8.36RR458 pKa = 11.84HH459 pKa = 5.43PYY461 pKa = 9.58RR462 pKa = 11.84RR463 pKa = 3.54

Molecular weight: 53.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12601

50

808

221.1

24.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.015 ± 0.424

0.651 ± 0.097

6.468 ± 0.307

6.484 ± 0.4

3.024 ± 0.226

8.039 ± 0.38

1.873 ± 0.196

4.857 ± 0.409

4.881 ± 0.398

8.46 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.124

3.531 ± 0.185

4.539 ± 0.225

3.952 ± 0.167

5.754 ± 0.358

5.928 ± 0.327

6.706 ± 0.299

7.428 ± 0.32

2.016 ± 0.205

2.944 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski