Beihai sobemo-like virus 19

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE63|A0A1L3KE63_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 19 OX=1922690 PE=4 SV=1
MM1 pKa = 7.99AIPSFIASMYY11 pKa = 10.93DD12 pKa = 3.25LASEE16 pKa = 4.11QFGVIGMLKK25 pKa = 10.51PNLTLNWDD33 pKa = 3.9YY34 pKa = 10.8FQSKK38 pKa = 10.55APTSPPEE45 pKa = 3.85VQTFAGQLLASIMVSVYY62 pKa = 10.96SLYY65 pKa = 10.94KK66 pKa = 8.42STDD69 pKa = 3.62MIWAFLVGIVTSLNPNIRR87 pKa = 11.84TSNLVRR93 pKa = 11.84PFTIDD98 pKa = 4.03KK99 pKa = 9.25ILSYY103 pKa = 11.13AGLCTCAYY111 pKa = 9.04WSYY114 pKa = 9.24TAEE117 pKa = 4.05NVIMAFFAVVGMCQSAKK134 pKa = 10.59LVIGHH139 pKa = 7.23LWWCKK144 pKa = 8.96NAWSASEE151 pKa = 4.71HH152 pKa = 6.31SDD154 pKa = 3.38QIAGNSYY161 pKa = 9.89FEE163 pKa = 5.22SMTADD168 pKa = 3.28SSIKK172 pKa = 10.48EE173 pKa = 4.08DD174 pKa = 3.5TKK176 pKa = 11.58KK177 pKa = 10.22MKK179 pKa = 9.89CQAMACAGPYY189 pKa = 8.94TGSPLVGQVIRR200 pKa = 11.84LPSFMDD206 pKa = 4.74DD207 pKa = 3.34GEE209 pKa = 4.8LYY211 pKa = 7.89CTPVHH216 pKa = 6.24VLNDD220 pKa = 4.07DD221 pKa = 3.39VTHH224 pKa = 6.63IVTHH228 pKa = 6.36KK229 pKa = 10.44SSVNIEE235 pKa = 4.31SVEE238 pKa = 3.95PLPPARR244 pKa = 11.84GFPDD248 pKa = 3.7LAFLKK253 pKa = 10.59LPNGTGAQLGMSEE266 pKa = 4.59AVVSSIMGGEE276 pKa = 3.91TGMAHH281 pKa = 6.85AMGVGSNFYY290 pKa = 9.63EE291 pKa = 4.29RR292 pKa = 11.84SSGRR296 pKa = 11.84IIEE299 pKa = 4.15LEE301 pKa = 4.02RR302 pKa = 11.84GGLAYY307 pKa = 10.59LGSTLPGWSGAPYY320 pKa = 9.57EE321 pKa = 4.29VGGKK325 pKa = 9.39VVGVHH330 pKa = 5.65HH331 pKa = 6.46VGIPSEE337 pKa = 4.29GNLGYY342 pKa = 10.72DD343 pKa = 3.23ILLAMKK349 pKa = 10.07ILEE352 pKa = 4.29KK353 pKa = 10.72QMQEE357 pKa = 3.54PEE359 pKa = 3.89YY360 pKa = 10.22EE361 pKa = 3.91KK362 pKa = 10.7RR363 pKa = 11.84KK364 pKa = 10.4RR365 pKa = 11.84GWKK368 pKa = 9.58KK369 pKa = 9.01KK370 pKa = 8.75FRR372 pKa = 11.84RR373 pKa = 11.84EE374 pKa = 3.9KK375 pKa = 11.06YY376 pKa = 8.49PVADD380 pKa = 3.2IRR382 pKa = 11.84ASNGYY387 pKa = 9.83AISEE391 pKa = 4.07HH392 pKa = 6.82RR393 pKa = 11.84IGGARR398 pKa = 11.84TDD400 pKa = 3.52IMWKK404 pKa = 9.72IKK406 pKa = 10.19NPKK409 pKa = 9.06GKK411 pKa = 9.55IVMLYY416 pKa = 10.4YY417 pKa = 10.71DD418 pKa = 5.03DD419 pKa = 3.98LTEE422 pKa = 4.23EE423 pKa = 4.35EE424 pKa = 4.39RR425 pKa = 11.84SHH427 pKa = 7.78IRR429 pKa = 11.84GEE431 pKa = 4.11MDD433 pKa = 2.56WQGYY437 pKa = 7.32SFEE440 pKa = 4.98SGPKK444 pKa = 8.17PTTPVNAGLIEE455 pKa = 3.96QVRR458 pKa = 11.84NNLRR462 pKa = 11.84AAQNAKK468 pKa = 10.08RR469 pKa = 11.84DD470 pKa = 3.96LQTMSDD476 pKa = 4.46LYY478 pKa = 11.06QLANIRR484 pKa = 11.84TRR486 pKa = 11.84NGPLEE491 pKa = 3.9TAAQVGVDD499 pKa = 3.67TQAINAAISEE509 pKa = 4.32LHH511 pKa = 5.89LQMEE515 pKa = 5.03RR516 pKa = 11.84FAEE519 pKa = 4.67LIEE522 pKa = 4.31YY523 pKa = 10.28YY524 pKa = 9.74SAKK527 pKa = 10.04SAEE530 pKa = 4.82DD531 pKa = 3.18IQNQKK536 pKa = 10.48RR537 pKa = 11.84MIRR540 pKa = 11.84KK541 pKa = 8.82KK542 pKa = 10.35RR543 pKa = 11.84NKK545 pKa = 9.45EE546 pKa = 3.77KK547 pKa = 10.45PLVSQEE553 pKa = 3.76VAAEE557 pKa = 4.07IQRR560 pKa = 11.84KK561 pKa = 7.49QADD564 pKa = 3.16INAALSRR571 pKa = 11.84AAHH574 pKa = 6.27LPPRR578 pKa = 11.84EE579 pKa = 3.92QGGQIDD585 pKa = 4.55DD586 pKa = 4.27EE587 pKa = 5.35NDD589 pKa = 3.13VEE591 pKa = 4.39MPAYY595 pKa = 9.24EE596 pKa = 4.26KK597 pKa = 10.86LSSHH601 pKa = 5.92FVGRR605 pKa = 11.84FKK607 pKa = 11.06DD608 pKa = 3.28KK609 pKa = 10.71YY610 pKa = 9.49GAIPRR615 pKa = 11.84EE616 pKa = 3.92FLDD619 pKa = 4.07DD620 pKa = 3.9CSSGEE625 pKa = 3.96EE626 pKa = 4.12SGEE629 pKa = 4.21EE630 pKa = 3.77EE631 pKa = 4.82DD632 pKa = 5.55SPTFSPTPSMEE643 pKa = 3.96KK644 pKa = 10.27SSLNVDD650 pKa = 3.04QDD652 pKa = 3.72ASSTSCQTTKK662 pKa = 10.8TDD664 pKa = 3.56VEE666 pKa = 4.4RR667 pKa = 11.84LKK669 pKa = 10.82HH670 pKa = 6.48LYY672 pKa = 8.96MSKK675 pKa = 9.92LHH677 pKa = 6.21SGQMEE682 pKa = 4.38ATSGPQMINQTSLNRR697 pKa = 11.84LKK699 pKa = 10.22NTFNEE704 pKa = 4.18DD705 pKa = 2.86MRR707 pKa = 11.84QLEE710 pKa = 4.59VASLPMTPKK719 pKa = 10.71LGRR722 pKa = 11.84LQSAFDD728 pKa = 3.66TSLGGSKK735 pKa = 8.07TQFNYY740 pKa = 9.87EE741 pKa = 3.61QSGKK745 pKa = 10.21KK746 pKa = 9.29KK747 pKa = 10.03RR748 pKa = 11.84KK749 pKa = 7.64SVHH752 pKa = 5.11WKK754 pKa = 8.1TSKK757 pKa = 10.26KK758 pKa = 10.25RR759 pKa = 11.84NSQTSSKK766 pKa = 10.59RR767 pKa = 11.84SLTRR771 pKa = 11.84NPVQEE776 pKa = 4.37SHH778 pKa = 6.68TSTKK782 pKa = 10.37DD783 pKa = 3.12KK784 pKa = 11.13QMQKK788 pKa = 10.37SLDD791 pKa = 3.61MSSSKK796 pKa = 9.53DD797 pKa = 3.44TQMSMASDD805 pKa = 3.89RR806 pKa = 11.84MQSTEE811 pKa = 3.83SGNN814 pKa = 3.38

Molecular weight: 90.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE63|A0A1L3KE63_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 19 OX=1922690 PE=4 SV=1
MM1 pKa = 7.32WRR3 pKa = 11.84LISGVSVRR11 pKa = 11.84DD12 pKa = 3.34QVVAHH17 pKa = 5.81YY18 pKa = 10.74LFGDD22 pKa = 3.47MMEE25 pKa = 4.47VLIEE29 pKa = 4.14HH30 pKa = 7.28PGLYY34 pKa = 8.59HH35 pKa = 7.12TMIGWSPFQRR45 pKa = 11.84AGQRR49 pKa = 11.84YY50 pKa = 6.85FHH52 pKa = 6.91HH53 pKa = 6.59LVKK56 pKa = 10.58FPAQSSDD63 pKa = 2.86KK64 pKa = 11.28SSWDD68 pKa = 2.88WTLQPWAVDD77 pKa = 3.14IFKK80 pKa = 10.97EE81 pKa = 4.4LMLWLHH87 pKa = 6.49DD88 pKa = 3.91HH89 pKa = 6.85RR90 pKa = 11.84KK91 pKa = 8.63PWTQKK96 pKa = 9.79VMEE99 pKa = 4.06NHH101 pKa = 6.07IEE103 pKa = 3.9AMLGQKK109 pKa = 8.31TFFLRR114 pKa = 11.84GEE116 pKa = 3.97VRR118 pKa = 11.84KK119 pKa = 9.21QQDD122 pKa = 3.28RR123 pKa = 11.84GLMPSGWKK131 pKa = 7.27MTLAGNSIFQIILHH145 pKa = 5.58VLAALRR151 pKa = 11.84AGVTVSLPMTMGDD164 pKa = 3.32DD165 pKa = 3.99TVGEE169 pKa = 4.37EE170 pKa = 4.69CPPEE174 pKa = 3.71YY175 pKa = 9.83WEE177 pKa = 3.92QYY179 pKa = 9.09KK180 pKa = 10.78RR181 pKa = 11.84LGAIVKK187 pKa = 9.05EE188 pKa = 4.52VSQPSNTEE196 pKa = 3.0IDD198 pKa = 3.56FCGMIFHH205 pKa = 7.7KK206 pKa = 10.84DD207 pKa = 3.15GTFVPAYY214 pKa = 9.16KK215 pKa = 9.9EE216 pKa = 3.54KK217 pKa = 10.6HH218 pKa = 5.34AFLVRR223 pKa = 11.84RR224 pKa = 11.84IKK226 pKa = 10.87SDD228 pKa = 3.47VLKK231 pKa = 8.88EE232 pKa = 4.09TLMSYY237 pKa = 10.48QLLYY241 pKa = 11.01VYY243 pKa = 9.3DD244 pKa = 3.75QPRR247 pKa = 11.84FDD249 pKa = 6.0AIQRR253 pKa = 11.84WMRR256 pKa = 11.84SIGHH260 pKa = 6.48GSMNFEE266 pKa = 3.75RR267 pKa = 11.84EE268 pKa = 3.52QLIRR272 pKa = 11.84HH273 pKa = 5.5LQGLRR278 pKa = 11.84TKK280 pKa = 10.48RR281 pKa = 11.84RR282 pKa = 11.84FVGLSRR288 pKa = 11.84LDD290 pKa = 3.26IRR292 pKa = 5.05

Molecular weight: 34.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1106

292

814

553.0

62.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.6 ± 1.158

0.995 ± 0.167

4.882 ± 0.137

6.42 ± 0.507

3.436 ± 0.917

6.962 ± 0.061

2.712 ± 0.938

5.154 ± 0.176

6.51 ± 0.556

7.957 ± 0.88

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.521 ± 0.332

3.526 ± 1.163

4.521 ± 0.222

5.244 ± 0.312

5.515 ± 1.089

9.132 ± 1.785

5.154 ± 0.563

5.606 ± 0.671

1.899 ± 0.823

3.255 ± 0.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski