Arthrobacter phage Franzy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marthavirus; unclassified Marthavirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222Z0P6|A0A222Z0P6_9CAUD Uncharacterized protein OS=Arthrobacter phage Franzy OX=2024007 GN=SEA_FRANZY_24 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 3.48GVLSFPFRR11 pKa = 11.84LDD13 pKa = 3.07TTGAIATVADD23 pKa = 4.2GSDD26 pKa = 3.18AYY28 pKa = 10.8VDD30 pKa = 3.56EE31 pKa = 5.3MIAKK35 pKa = 10.07LVLTNIGEE43 pKa = 4.61CPMSPEE49 pKa = 4.09YY50 pKa = 10.59GVPDD54 pKa = 3.61PTFAALHH61 pKa = 6.3IGDD64 pKa = 4.04VQAGLSTFGPAGVRR78 pKa = 11.84VTAVEE83 pKa = 4.0MTPYY87 pKa = 10.97SDD89 pKa = 3.67TQSVASIAWAYY100 pKa = 10.0EE101 pKa = 3.61NTLGVTNNGG110 pKa = 3.33

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222Z0V9|A0A222Z0V9_9CAUD Uncharacterized protein OS=Arthrobacter phage Franzy OX=2024007 GN=SEA_FRANZY_71 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.45KK3 pKa = 10.16EE4 pKa = 3.66IAIGLGVMLLAASTFALSAHH24 pKa = 5.54QSVTVVLLIVLVSSLTVLTVYY45 pKa = 10.47YY46 pKa = 9.58MRR48 pKa = 11.84RR49 pKa = 11.84ARR51 pKa = 11.84WKK53 pKa = 8.3QYY55 pKa = 7.99TSGRR59 pKa = 11.84VFLYY63 pKa = 9.67QLWAFDD69 pKa = 3.77ALIVYY74 pKa = 8.37WLFSRR79 pKa = 11.84LITDD83 pKa = 3.43RR84 pKa = 11.84DD85 pKa = 3.01IRR87 pKa = 11.84IFVFNVLIAGLIAAFWLITGTFWKK111 pKa = 9.74AQKK114 pKa = 9.76HH115 pKa = 4.88ARR117 pKa = 11.84TEE119 pKa = 3.87RR120 pKa = 11.84LRR122 pKa = 11.84RR123 pKa = 11.84AEE125 pKa = 3.99TTEE128 pKa = 3.88KK129 pKa = 10.98KK130 pKa = 9.87EE131 pKa = 4.07LKK133 pKa = 10.44

Molecular weight: 15.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

15706

42

876

218.1

23.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.377 ± 0.558

0.611 ± 0.105

6.233 ± 0.226

6.424 ± 0.379

2.782 ± 0.176

7.691 ± 0.398

1.579 ± 0.111

4.642 ± 0.243

4.38 ± 0.236

8.099 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.121

3.234 ± 0.185

5.304 ± 0.308

3.686 ± 0.198

6.488 ± 0.384

5.431 ± 0.247

6.845 ± 0.364

7.793 ± 0.317

1.534 ± 0.148

2.515 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski