Staphylococcus phage P282

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus P282

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9H054|A0A1X9H054_9CAUD Uncharacterized protein OS=Staphylococcus phage P282 OX=1735645 GN=P282_60 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.45VEE20 pKa = 3.82YY21 pKa = 10.92QHH23 pKa = 6.88FDD25 pKa = 3.86DD26 pKa = 6.2VDD28 pKa = 3.79DD29 pKa = 5.21EE30 pKa = 4.86KK31 pKa = 11.62DD32 pKa = 3.23ALADD36 pKa = 3.54YY37 pKa = 10.65LYY39 pKa = 11.0NNPNEE44 pKa = 3.8ILEE47 pKa = 3.94YY48 pKa = 11.1DD49 pKa = 3.47NLKK52 pKa = 9.73IRR54 pKa = 11.84NVNIEE59 pKa = 3.84VEE61 pKa = 4.2

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9H0J2|A0A1X9H0J2_9CAUD Uncharacterized protein OS=Staphylococcus phage P282 OX=1735645 GN=P282_11 PE=4 SV=1
MM1 pKa = 7.13VNKK4 pKa = 9.9INKK7 pKa = 8.26FNQYY11 pKa = 6.91VARR14 pKa = 11.84FIPYY18 pKa = 9.11IATKK22 pKa = 10.35IPIKK26 pKa = 10.71KK27 pKa = 10.0NIAPMIKK34 pKa = 9.64QKK36 pKa = 11.1SDD38 pKa = 3.15AEE40 pKa = 4.16LLKK43 pKa = 11.22NEE45 pKa = 4.09VFRR48 pKa = 11.84VRR50 pKa = 11.84KK51 pKa = 9.44

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12187

45

1509

179.2

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.621 ± 0.47

0.542 ± 0.105

6.171 ± 0.353

8.296 ± 0.567

4.25 ± 0.366

5.67 ± 0.627

1.641 ± 0.158

8.074 ± 0.307

9.773 ± 0.384

7.705 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.2

6.54 ± 0.287

2.626 ± 0.171

3.528 ± 0.232

4.3 ± 0.257

5.744 ± 0.289

5.432 ± 0.259

5.744 ± 0.231

1.116 ± 0.158

4.652 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski