Amino acid dipepetide frequency for Salpingoeca rosetta (strain ATCC 50818 / BSB-021)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.964AlaAla: 13.964 ± 0.103	1.92AlaCys: 1.92 ± 0.025	5.728AlaAsp: 5.728 ± 0.035	5.17AlaGlu: 5.17 ± 0.042	3.188AlaPhe: 3.188 ± 0.023	6.601AlaGly: 6.601 ± 0.048	2.843AlaHis: 2.843 ± 0.025	3.5AlaIle: 3.5 ± 0.025	3.825AlaLys: 3.825 ± 0.036	8.646AlaLeu: 8.646 ± 0.053	2.294AlaMet: 2.294 ± 0.019	2.939AlaAsn: 2.939 ± 0.024	5.314AlaPro: 5.314 ± 0.045	4.323AlaGln: 4.323 ± 0.034	6.268AlaArg: 6.268 ± 0.034	8.808AlaSer: 8.808 ± 0.049	7.98AlaThr: 7.98 ± 0.058	7.41AlaVal: 7.41 ± 0.046	1.063AlaTrp: 1.063 ± 0.014	1.849AlaTyr: 1.849 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	1.535CysAla: 1.535 ± 0.023	0.439CysCys: 0.439 ± 0.01	1.156CysAsp: 1.156 ± 0.023	0.878CysGlu: 0.878 ± 0.018	0.604CysPhe: 0.604 ± 0.009	1.251CysGly: 1.251 ± 0.021	0.467CysHis: 0.467 ± 0.01	0.699CysIle: 0.699 ± 0.012	0.661CysLys: 0.661 ± 0.012	1.41CysLeu: 1.41 ± 0.019	0.408CysMet: 0.408 ± 0.008	0.603CysAsn: 0.603 ± 0.014	0.942CysPro: 0.942 ± 0.036	0.586CysGln: 0.586 ± 0.013	1.062CysArg: 1.062 ± 0.015	1.309CysSer: 1.309 ± 0.023	1.314CysThr: 1.314 ± 0.026	1.411CysVal: 1.411 ± 0.021	0.193CysTrp: 0.193 ± 0.006	0.342CysTyr: 0.342 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	6.909AspAla: 6.909 ± 0.046	0.853AspCys: 0.853 ± 0.017	8.728AspAsp: 8.728 ± 0.116	4.766AspGlu: 4.766 ± 0.034	1.801AspPhe: 1.801 ± 0.019	6.111AspGly: 6.111 ± 0.052	1.461AspHis: 1.461 ± 0.015	2.311AspIle: 2.311 ± 0.018	2.194AspLys: 2.194 ± 0.025	4.548AspLeu: 4.548 ± 0.034	1.419AspMet: 1.419 ± 0.018	2.332AspAsn: 2.332 ± 0.025	2.54AspPro: 2.54 ± 0.023	1.727AspGln: 1.727 ± 0.013	2.952AspArg: 2.952 ± 0.029	3.697AspSer: 3.697 ± 0.027	4.084AspThr: 4.084 ± 0.03	5.045AspVal: 5.045 ± 0.036	0.545AspTrp: 0.545 ± 0.01	1.146AspTyr: 1.146 ± 0.014	0.0AspXaa: 0.0 ± 0.0
Glu	5.577GluAla: 5.577 ± 0.042	0.974GluCys: 0.974 ± 0.024	4.216GluAsp: 4.216 ± 0.035	6.301GluGlu: 6.301 ± 0.083	1.552GluPhe: 1.552 ± 0.018	3.552GluGly: 3.552 ± 0.027	1.859GluHis: 1.859 ± 0.02	1.593GluIle: 1.593 ± 0.02	2.467GluLys: 2.467 ± 0.035	4.874GluLeu: 4.874 ± 0.038	1.213GluMet: 1.213 ± 0.015	1.415GluAsn: 1.415 ± 0.017	2.106GluPro: 2.106 ± 0.02	3.499GluGln: 3.499 ± 0.035	3.822GluArg: 3.822 ± 0.04	2.845GluSer: 2.845 ± 0.023	2.926GluThr: 2.926 ± 0.023	3.385GluVal: 3.385 ± 0.027	0.605GluTrp: 0.605 ± 0.009	1.336GluTyr: 1.336 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	2.97PheAla: 2.97 ± 0.023	0.523PheCys: 0.523 ± 0.009	2.208PheAsp: 2.208 ± 0.023	1.83PheGlu: 1.83 ± 0.019	1.243PhePhe: 1.243 ± 0.017	2.348PheGly: 2.348 ± 0.029	0.865PheHis: 0.865 ± 0.013	1.139PheIle: 1.139 ± 0.015	1.278PheLys: 1.278 ± 0.015	2.704PheLeu: 2.704 ± 0.025	0.645PheMet: 0.645 ± 0.01	1.119PheAsn: 1.119 ± 0.016	1.4PhePro: 1.4 ± 0.015	1.102PheGln: 1.102 ± 0.013	1.631PheArg: 1.631 ± 0.015	2.339PheSer: 2.339 ± 0.025	1.886PheThr: 1.886 ± 0.021	2.508PheVal: 2.508 ± 0.025	0.34PheTrp: 0.34 ± 0.006	0.745PheTyr: 0.745 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	6.406GlyAla: 6.406 ± 0.047	1.134GlyCys: 1.134 ± 0.02	5.899GlyAsp: 5.899 ± 0.047	3.635GlyGlu: 3.635 ± 0.03	2.002GlyPhe: 2.002 ± 0.018	8.077GlyGly: 8.077 ± 0.086	1.881GlyHis: 1.881 ± 0.022	2.153GlyIle: 2.153 ± 0.02	2.585GlyLys: 2.585 ± 0.025	4.513GlyLeu: 4.513 ± 0.034	1.369GlyMet: 1.369 ± 0.018	2.382GlyAsn: 2.382 ± 0.023	2.363GlyPro: 2.363 ± 0.03	2.438GlyGln: 2.438 ± 0.027	4.274GlyArg: 4.274 ± 0.037	5.618GlySer: 5.618 ± 0.049	4.256GlyThr: 4.256 ± 0.046	4.897GlyVal: 4.897 ± 0.033	0.673GlyTrp: 0.673 ± 0.011	1.402GlyTyr: 1.402 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	3.25HisAla: 3.25 ± 0.03	0.445HisCys: 0.445 ± 0.008	1.991HisAsp: 1.991 ± 0.02	1.557HisGlu: 1.557 ± 0.018	0.863HisPhe: 0.863 ± 0.013	2.137HisGly: 2.137 ± 0.025	1.976HisHis: 1.976 ± 0.035	1.155HisIle: 1.155 ± 0.013	1.083HisLys: 1.083 ± 0.015	2.513HisLeu: 2.513 ± 0.025	0.755HisMet: 0.755 ± 0.011	1.085HisAsn: 1.085 ± 0.015	1.683HisPro: 1.683 ± 0.021	1.925HisGln: 1.925 ± 0.029	2.12HisArg: 2.12 ± 0.02	1.895HisSer: 1.895 ± 0.018	2.08HisThr: 2.08 ± 0.023	2.456HisVal: 2.456 ± 0.023	0.29HisTrp: 0.29 ± 0.007	0.589HisTyr: 0.589 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.496IleAla: 3.496 ± 0.024	0.542IleCys: 0.542 ± 0.009	2.267IleAsp: 2.267 ± 0.024	1.823IleGlu: 1.823 ± 0.021	1.112IlePhe: 1.112 ± 0.017	2.032IleGly: 2.032 ± 0.02	0.923IleHis: 0.923 ± 0.013	1.361IleIle: 1.361 ± 0.02	1.476IleLys: 1.476 ± 0.015	2.458IleLeu: 2.458 ± 0.023	0.688IleMet: 0.688 ± 0.012	1.323IleAsn: 1.323 ± 0.016	1.643IlePro: 1.643 ± 0.019	1.182IleGln: 1.182 ± 0.013	1.936IleArg: 1.936 ± 0.017	2.431IleSer: 2.431 ± 0.02	2.496IleThr: 2.496 ± 0.026	2.593IleVal: 2.593 ± 0.026	0.289IleTrp: 0.289 ± 0.006	0.717IleTyr: 0.717 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	3.836LysAla: 3.836 ± 0.037	0.545LysCys: 0.545 ± 0.011	2.209LysAsp: 2.209 ± 0.025	2.68LysGlu: 2.68 ± 0.037	1.007LysPhe: 1.007 ± 0.013	2.309LysGly: 2.309 ± 0.028	1.426LysHis: 1.426 ± 0.019	1.082LysIle: 1.082 ± 0.015	3.004LysLys: 3.004 ± 0.048	3.221LysLeu: 3.221 ± 0.029	0.814LysMet: 0.814 ± 0.01	1.084LysAsn: 1.084 ± 0.014	1.99LysPro: 1.99 ± 0.024	2.553LysGln: 2.553 ± 0.034	3.119LysArg: 3.119 ± 0.03	2.246LysSer: 2.246 ± 0.026	2.236LysThr: 2.236 ± 0.023	2.049LysVal: 2.049 ± 0.021	0.476LysTrp: 0.476 ± 0.012	0.907LysTyr: 0.907 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	8.11LeuAla: 8.11 ± 0.047	1.554LeuCys: 1.554 ± 0.015	4.859LeuAsp: 4.859 ± 0.033	4.701LeuGlu: 4.701 ± 0.042	2.83LeuPhe: 2.83 ± 0.029	4.508LeuGly: 4.508 ± 0.029	2.907LeuHis: 2.907 ± 0.029	2.6LeuIle: 2.6 ± 0.028	3.48LeuLys: 3.48 ± 0.032	8.52LeuLeu: 8.52 ± 0.059	1.675LeuMet: 1.675 ± 0.016	2.522LeuAsn: 2.522 ± 0.023	4.809LeuPro: 4.809 ± 0.038	4.63LeuGln: 4.63 ± 0.04	6.086LeuArg: 6.086 ± 0.037	6.505LeuSer: 6.505 ± 0.044	4.89LeuThr: 4.89 ± 0.049	5.506LeuVal: 5.506 ± 0.039	0.881LeuTrp: 0.881 ± 0.013	1.763LeuTyr: 1.763 ± 0.015	0.0LeuXaa: 0.0 ± 0.0
Met	2.185MetAla: 2.185 ± 0.022	0.419MetCys: 0.419 ± 0.008	1.24MetAsp: 1.24 ± 0.013	1.169MetGlu: 1.169 ± 0.014	0.656MetPhe: 0.656 ± 0.009	1.114MetGly: 1.114 ± 0.013	0.728MetHis: 0.728 ± 0.01	0.589MetIle: 0.589 ± 0.01	0.825MetLys: 0.825 ± 0.013	2.016MetLeu: 2.016 ± 0.018	0.714MetMet: 0.714 ± 0.012	0.576MetAsn: 0.576 ± 0.01	1.146MetPro: 1.146 ± 0.016	1.201MetGln: 1.201 ± 0.014	1.466MetArg: 1.466 ± 0.014	1.604MetSer: 1.604 ± 0.015	1.373MetThr: 1.373 ± 0.013	1.188MetVal: 1.188 ± 0.014	0.251MetTrp: 0.251 ± 0.006	0.472MetTyr: 0.472 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	3.225AsnAla: 3.225 ± 0.032	0.487AsnCys: 0.487 ± 0.012	2.185AsnAsp: 2.185 ± 0.028	1.515AsnGlu: 1.515 ± 0.017	0.91AsnPhe: 0.91 ± 0.013	2.628AsnGly: 2.628 ± 0.029	0.802AsnHis: 0.802 ± 0.011	1.305AsnIle: 1.305 ± 0.016	1.368AsnLys: 1.368 ± 0.018	2.343AsnLeu: 2.343 ± 0.025	0.721AsnMet: 0.721 ± 0.012	1.95AsnAsn: 1.95 ± 0.032	1.599AsnPro: 1.599 ± 0.016	1.001AsnGln: 1.001 ± 0.013	1.555AsnArg: 1.555 ± 0.017	2.343AsnSer: 2.343 ± 0.022	2.618AsnThr: 2.618 ± 0.028	2.154AsnVal: 2.154 ± 0.022	0.264AsnTrp: 0.264 ± 0.006	0.61AsnTyr: 0.61 ± 0.011	0.0AsnXaa: 0.0 ± 0.0
Pro	5.775ProAla: 5.775 ± 0.048	0.843ProCys: 0.843 ± 0.024	2.617ProAsp: 2.617 ± 0.018	2.465ProGlu: 2.465 ± 0.024	1.604ProPhe: 1.604 ± 0.017	2.87ProGly: 2.87 ± 0.033	1.705ProHis: 1.705 ± 0.021	1.477ProIle: 1.477 ± 0.018	1.75ProLys: 1.75 ± 0.019	4.227ProLeu: 4.227 ± 0.03	0.919ProMet: 0.919 ± 0.012	1.473ProAsn: 1.473 ± 0.013	5.119ProPro: 5.119 ± 0.067	2.651ProGln: 2.651 ± 0.031	3.192ProArg: 3.192 ± 0.022	5.273ProSer: 5.273 ± 0.039	4.0ProThr: 4.0 ± 0.038	3.191ProVal: 3.191 ± 0.026	0.507ProTrp: 0.507 ± 0.009	1.005ProTyr: 1.005 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	4.494GlnAla: 4.494 ± 0.034	0.716GlnCys: 0.716 ± 0.015	2.234GlnAsp: 2.234 ± 0.02	3.015GlnGlu: 3.015 ± 0.033	1.122GlnPhe: 1.122 ± 0.013	2.561GlnGly: 2.561 ± 0.022	2.851GlnHis: 2.851 ± 0.037	1.01GlnIle: 1.01 ± 0.013	1.404GlnLys: 1.404 ± 0.022	4.595GlnLeu: 4.595 ± 0.036	0.842GlnMet: 0.842 ± 0.012	0.91GlnAsn: 0.91 ± 0.011	3.227GlnPro: 3.227 ± 0.035	12.56GlnGln: 12.56 ± 0.211	4.194GlnArg: 4.194 ± 0.036	2.721GlnSer: 2.721 ± 0.022	2.14GlnThr: 2.14 ± 0.018	2.413GlnVal: 2.413 ± 0.02	0.465GlnTrp: 0.465 ± 0.008	0.962GlnTyr: 0.962 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	6.319ArgAla: 6.319 ± 0.039	1.069ArgCys: 1.069 ± 0.016	3.574ArgAsp: 3.574 ± 0.03	3.518ArgGlu: 3.518 ± 0.033	2.022ArgPhe: 2.022 ± 0.019	4.238ArgGly: 4.238 ± 0.043	2.253ArgHis: 2.253 ± 0.024	2.219ArgIle: 2.219 ± 0.02	2.796ArgLys: 2.796 ± 0.03	5.591ArgLeu: 5.591 ± 0.039	1.366ArgMet: 1.366 ± 0.014	1.896ArgAsn: 1.896 ± 0.017	3.134ArgPro: 3.134 ± 0.028	3.5ArgGln: 3.5 ± 0.03	6.357ArgArg: 6.357 ± 0.054	4.624ArgSer: 4.624 ± 0.034	3.813ArgThr: 3.813 ± 0.028	4.584ArgVal: 4.584 ± 0.034	0.707ArgTrp: 0.707 ± 0.011	1.264ArgTyr: 1.264 ± 0.013	0.0ArgXaa: 0.0 ± 0.0
Ser	7.738SerAla: 7.738 ± 0.044	1.24SerCys: 1.24 ± 0.024	4.305SerAsp: 4.305 ± 0.033	3.062SerGlu: 3.062 ± 0.025	2.416SerPhe: 2.416 ± 0.024	5.696SerGly: 5.696 ± 0.044	1.998SerHis: 1.998 ± 0.018	2.585SerIle: 2.585 ± 0.024	2.921SerLys: 2.921 ± 0.029	6.144SerLeu: 6.144 ± 0.041	1.567SerMet: 1.567 ± 0.015	2.7SerAsn: 2.7 ± 0.025	4.549SerPro: 4.549 ± 0.036	2.805SerGln: 2.805 ± 0.025	4.566SerArg: 4.566 ± 0.032	11.446SerSer: 11.446 ± 0.101	6.737SerThr: 6.737 ± 0.087	4.726SerVal: 4.726 ± 0.043	0.701SerTrp: 0.701 ± 0.011	1.275SerTyr: 1.275 ± 0.016	0.0SerXaa: 0.0 ± 0.0
Thr	8.615ThrAla: 8.615 ± 0.058	1.391ThrCys: 1.391 ± 0.028	3.226ThrAsp: 3.226 ± 0.033	2.633ThrGlu: 2.633 ± 0.022	2.181ThrPhe: 2.181 ± 0.025	4.036ThrGly: 4.036 ± 0.037	1.877ThrHis: 1.877 ± 0.021	2.451ThrIle: 2.451 ± 0.027	2.224ThrLys: 2.224 ± 0.024	5.403ThrLeu: 5.403 ± 0.044	1.359ThrMet: 1.359 ± 0.013	2.098ThrAsn: 2.098 ± 0.022	4.396ThrPro: 4.396 ± 0.04	2.602ThrGln: 2.602 ± 0.023	4.037ThrArg: 4.037 ± 0.026	6.43ThrSer: 6.43 ± 0.082	9.594ThrThr: 9.594 ± 0.24	4.144ThrVal: 4.144 ± 0.056	0.674ThrTrp: 0.674 ± 0.011	1.325ThrTyr: 1.325 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	6.677ValAla: 6.677 ± 0.043	1.507ValCys: 1.507 ± 0.025	4.482ValAsp: 4.482 ± 0.035	3.619ValGlu: 3.619 ± 0.028	2.52ValPhe: 2.52 ± 0.022	3.974ValGly: 3.974 ± 0.029	2.061ValHis: 2.061 ± 0.018	2.378ValIle: 2.378 ± 0.024	2.256ValLys: 2.256 ± 0.023	6.764ValLeu: 6.764 ± 0.048	1.396ValMet: 1.396 ± 0.014	2.046ValAsn: 2.046 ± 0.029	3.436ValPro: 3.436 ± 0.028	2.844ValGln: 2.844 ± 0.027	4.17ValArg: 4.17 ± 0.03	4.961ValSer: 4.961 ± 0.043	4.228ValThr: 4.228 ± 0.047	5.935ValVal: 5.935 ± 0.058	0.763ValTrp: 0.763 ± 0.011	1.615ValTyr: 1.615 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	0.781TrpAla: 0.781 ± 0.011	0.24TrpCys: 0.24 ± 0.006	0.566TrpAsp: 0.566 ± 0.009	0.53TrpGlu: 0.53 ± 0.009	0.394TrpPhe: 0.394 ± 0.008	0.474TrpGly: 0.474 ± 0.009	0.337TrpHis: 0.337 ± 0.007	0.366TrpIle: 0.366 ± 0.008	0.426TrpLys: 0.426 ± 0.007	1.11TrpLeu: 1.11 ± 0.015	0.277TrpMet: 0.277 ± 0.006	0.344TrpAsn: 0.344 ± 0.007	0.442TrpPro: 0.442 ± 0.015	0.536TrpGln: 0.536 ± 0.01	0.804TrpArg: 0.804 ± 0.013	0.768TrpSer: 0.768 ± 0.012	0.673TrpThr: 0.673 ± 0.01	0.582TrpVal: 0.582 ± 0.009	0.192TrpTrp: 0.192 ± 0.005	0.276TrpTyr: 0.276 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.773TyrAla: 1.773 ± 0.017	0.428TyrCys: 0.428 ± 0.014	1.479TyrAsp: 1.479 ± 0.019	1.246TyrGlu: 1.246 ± 0.018	0.795TyrPhe: 0.795 ± 0.014	1.443TyrGly: 1.443 ± 0.018	0.626TyrHis: 0.626 ± 0.009	0.818TyrIle: 0.818 ± 0.011	0.772TyrLys: 0.772 ± 0.013	1.735TyrLeu: 1.735 ± 0.019	0.49TyrMet: 0.49 ± 0.008	0.784TyrAsn: 0.784 ± 0.012	0.825TyrPro: 0.825 ± 0.011	0.773TyrGln: 0.773 ± 0.011	1.233TyrArg: 1.233 ± 0.014	1.313TyrSer: 1.313 ± 0.016	1.371TyrThr: 1.371 ± 0.023	1.456TyrVal: 1.456 ± 0.02	0.238TyrTrp: 0.238 ± 0.006	0.592TyrTyr: 0.592 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11698 proteins (7789328 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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