Shahe picorna-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KJ24|A0A1L3KJ24_9VIRU SF3 helicase domain-containing protein OS=Shahe picorna-like virus 12 OX=1923442 PE=4 SV=1
MM1 pKa = 7.6NYY3 pKa = 10.49DD4 pKa = 3.51QFLHH8 pKa = 6.55KK9 pKa = 10.21PLFIDD14 pKa = 3.33NFNWSTVDD22 pKa = 3.13AAGINIGGVQIPRR35 pKa = 11.84DD36 pKa = 3.48IFNTNSFLAIPWKK49 pKa = 10.02SSCYY53 pKa = 9.88YY54 pKa = 9.76RR55 pKa = 11.84LKK57 pKa = 10.74GKK59 pKa = 10.13LVIQVAGTVRR69 pKa = 11.84HH70 pKa = 5.85SGILLASALPFVAPTNFPLNSMLAVPHH97 pKa = 6.31AFVYY101 pKa = 10.82ANQSNPAIVEE111 pKa = 3.95IPFYY115 pKa = 10.79NPSPLRR121 pKa = 11.84QTQDD125 pKa = 2.6NDD127 pKa = 3.98RR128 pKa = 11.84TCHH131 pKa = 6.7LAIQRR136 pKa = 11.84DD137 pKa = 4.01GSDD140 pKa = 3.09SYY142 pKa = 11.86SDD144 pKa = 3.37VFIRR148 pKa = 11.84IINPLSAPTGGSTTVVVTVHH168 pKa = 6.54MILDD172 pKa = 3.66EE173 pKa = 4.65LEE175 pKa = 4.81FYY177 pKa = 10.54TPAPVDD183 pKa = 3.16IVYY186 pKa = 9.99AQSKK190 pKa = 10.41VITQAMDD197 pKa = 3.29SVASILKK204 pKa = 9.85QKK206 pKa = 10.76SSDD209 pKa = 3.7YY210 pKa = 10.39IDD212 pKa = 3.61NVRR215 pKa = 11.84GALHH219 pKa = 5.79QWTGLHH225 pKa = 6.12NPNIDD230 pKa = 3.37APLNKK235 pKa = 9.95HH236 pKa = 6.33FMQTRR241 pKa = 11.84ANPNVIDD248 pKa = 4.68AVTTYY253 pKa = 8.96DD254 pKa = 3.39TLHH257 pKa = 7.55PYY259 pKa = 10.15TMDD262 pKa = 3.29EE263 pKa = 4.35SGTTSAAFFHH273 pKa = 6.59TEE275 pKa = 3.61TDD277 pKa = 3.61EE278 pKa = 4.33MDD280 pKa = 3.3MKK282 pKa = 11.0YY283 pKa = 10.51LLSIPQVISTRR294 pKa = 11.84IPIPLNSAVGSQLFCRR310 pKa = 11.84PITPFMGVKK319 pKa = 10.48NSVTDD324 pKa = 3.18LHH326 pKa = 6.45TIKK329 pKa = 10.67SIQSKK334 pKa = 10.05LAFCASHH341 pKa = 6.63WSGDD345 pKa = 3.84MEE347 pKa = 6.1LMLQVDD353 pKa = 4.05MSNTQFLKK361 pKa = 11.04LLVVLDD367 pKa = 3.46YY368 pKa = 10.8TRR370 pKa = 11.84NINSTNLGIAGSPASFSPYY389 pKa = 10.12QGTLTHH395 pKa = 6.05TLEE398 pKa = 4.29FAGGGSIQCVTLPFMSAFRR417 pKa = 11.84QLPLTTDD424 pKa = 2.51WRR426 pKa = 11.84ANALSHH432 pKa = 5.95GLVRR436 pKa = 11.84IYY438 pKa = 10.78VLQPPVVSDD447 pKa = 3.81DD448 pKa = 3.73VAVPEE453 pKa = 4.23LNVYY457 pKa = 7.81YY458 pKa = 10.27RR459 pKa = 11.84CKK461 pKa = 10.92EE462 pKa = 3.73NFQLYY467 pKa = 8.85GFNYY471 pKa = 9.71RR472 pKa = 11.84RR473 pKa = 11.84GHH475 pKa = 5.75YY476 pKa = 9.87SATSPIPPIAAMGEE490 pKa = 4.16EE491 pKa = 4.68VLDD494 pKa = 4.06SMTTQSMAMSDD505 pKa = 3.44SSMLDD510 pKa = 3.01TTTSQEE516 pKa = 4.22CVTDD520 pKa = 3.89KK521 pKa = 11.27GPSKK525 pKa = 10.39PSVVYY530 pKa = 10.62GPGNLRR536 pKa = 11.84PIVSVRR542 pKa = 11.84DD543 pKa = 3.8IIRR546 pKa = 11.84RR547 pKa = 11.84VYY549 pKa = 9.64PVYY552 pKa = 11.2SNVLTPSVTSNVNYY566 pKa = 10.65LNLPLQGLFRR576 pKa = 11.84SRR578 pKa = 11.84QIGTNFSGTASDD590 pKa = 3.98ATPLSIISDD599 pKa = 3.49MFYY602 pKa = 11.13GFRR605 pKa = 11.84GSMKK609 pKa = 10.16LKK611 pKa = 10.7IMITGANASAVHH623 pKa = 6.56FYY625 pKa = 10.14PPNVSVTSEE634 pKa = 4.14GVNKK638 pKa = 9.64VYY640 pKa = 10.7TMTTPSTTNTTFIADD655 pKa = 3.9EE656 pKa = 4.39VGSCLPFSDD665 pKa = 4.6SFRR668 pKa = 11.84MIPGTTYY675 pKa = 11.02QEE677 pKa = 3.77MSDD680 pKa = 3.6FNRR683 pKa = 11.84RR684 pKa = 11.84PSIVHH689 pKa = 4.84TTTGVGSLADD699 pKa = 3.46SVSIHH704 pKa = 7.29DD705 pKa = 3.21IHH707 pKa = 8.23VPYY710 pKa = 9.91MSHH713 pKa = 6.85QDD715 pKa = 3.37FVTNDD720 pKa = 3.2GKK722 pKa = 11.28LLGLNTPQSSSDD734 pKa = 3.3SLGNLKK740 pKa = 9.97ISYY743 pKa = 8.78TPVIVSPAGATVVLSRR759 pKa = 11.84VHH761 pKa = 4.74ITIFVGLGDD770 pKa = 3.87DD771 pKa = 4.04ARR773 pKa = 11.84FGMLINARR781 pKa = 11.84EE782 pKa = 4.09LNPYY786 pKa = 9.24FALDD790 pKa = 3.74GVTKK794 pKa = 10.41IQLDD798 pKa = 4.74PIQHH802 pKa = 6.31ANVVVGNTTVPSLISDD818 pKa = 3.94SGAAQAYY825 pKa = 9.45IGG827 pKa = 3.94

Molecular weight:
90.43 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KJ24|A0A1L3KJ24_9VIRU SF3 helicase domain-containing protein OS=Shahe picorna-like virus 12 OX=1923442 PE=4 SV=1
MM1 pKa = 7.46VYY3 pKa = 10.61NSSTNQGDD11 pKa = 3.74RR12 pKa = 11.84KK13 pKa = 10.6NGNKK17 pKa = 9.73NFLFINNCFSNYY29 pKa = 9.93FIMANPYY36 pKa = 9.26RR37 pKa = 11.84CVRR40 pKa = 11.84SSKK43 pKa = 10.68NAFSSNEE50 pKa = 3.91WPVLKK55 pKa = 10.68CGLLLVKK62 pKa = 9.29FNPAAGSRR70 pKa = 11.84ILYY73 pKa = 10.04RR74 pKa = 11.84EE75 pKa = 3.87TFIQSVLIHH84 pKa = 5.99VDD86 pKa = 3.02RR87 pKa = 11.84MRR89 pKa = 11.84VQGLTDD95 pKa = 4.58IISDD99 pKa = 3.67MKK101 pKa = 10.57EE102 pKa = 3.7LLVSVFGVFRR112 pKa = 11.84TMVKK116 pKa = 10.15VVQKK120 pKa = 10.08CSNMKK125 pKa = 9.04TATALMDD132 pKa = 3.67WLLDD136 pKa = 4.6AILLLSDD143 pKa = 3.19VLTRR147 pKa = 11.84RR148 pKa = 11.84KK149 pKa = 10.17VYY151 pKa = 10.32GMNIIITLLRR161 pKa = 11.84VFKK164 pKa = 10.43LYY166 pKa = 10.45KK167 pKa = 10.26DD168 pKa = 4.18SPLHH172 pKa = 4.89PQSWQGLLVSLVSFGVPAAVIDD194 pKa = 3.93VVKK197 pKa = 10.78RR198 pKa = 11.84MSLLSNTKK206 pKa = 10.09VLDD209 pKa = 4.21DD210 pKa = 4.5FSLFHH215 pKa = 6.4QLISVCMDD223 pKa = 3.82TLASFIEE230 pKa = 4.46TLGFTQTPVVKK241 pKa = 10.41QIKK244 pKa = 9.9EE245 pKa = 3.9YY246 pKa = 10.86GKK248 pKa = 10.45DD249 pKa = 3.63LIQHH253 pKa = 6.64HH254 pKa = 6.54SLLADD259 pKa = 3.29IKK261 pKa = 11.35DD262 pKa = 3.39MCKK265 pKa = 9.96LVKK268 pKa = 9.5KK269 pKa = 10.24DD270 pKa = 3.35RR271 pKa = 11.84RR272 pKa = 11.84IFSEE276 pKa = 3.79SHH278 pKa = 4.68FRR280 pKa = 11.84EE281 pKa = 4.65KK282 pKa = 10.6IFTTHH287 pKa = 5.94EE288 pKa = 4.18KK289 pKa = 10.8VVGNTILLDD298 pKa = 3.46WGKK301 pKa = 8.73TSPSVANEE309 pKa = 3.12IATIKK314 pKa = 10.2EE315 pKa = 4.16LNKK318 pKa = 10.39LALAYY323 pKa = 9.28EE324 pKa = 4.33NNSRR328 pKa = 11.84QEE330 pKa = 4.02PLCMCFEE337 pKa = 4.57GPPGVFKK344 pKa = 10.49STIMSLLLKK353 pKa = 10.36ALNKK357 pKa = 9.97SVYY360 pKa = 8.41SHH362 pKa = 5.5QVKK365 pKa = 10.72SLMDD369 pKa = 3.69GKK371 pKa = 10.44DD372 pKa = 3.51FYY374 pKa = 11.56DD375 pKa = 3.62QYY377 pKa = 11.83DD378 pKa = 3.65NQDD381 pKa = 3.0IMLMDD386 pKa = 4.86DD387 pKa = 4.51LGAGGDD393 pKa = 3.63SQFRR397 pKa = 11.84FLVNLISPIKK407 pKa = 10.48YY408 pKa = 9.72PLDD411 pKa = 3.92CASEE415 pKa = 4.16KK416 pKa = 11.19LKK418 pKa = 9.8DD419 pKa = 3.55TKK421 pKa = 10.76FFTSEE426 pKa = 3.62AVLFTTNKK434 pKa = 9.56FSTIRR439 pKa = 11.84STTSGSGIVDD449 pKa = 4.0LNAVKK454 pKa = 10.41RR455 pKa = 11.84RR456 pKa = 11.84VVLIDD461 pKa = 3.88FSHH464 pKa = 6.64VKK466 pKa = 10.44RR467 pKa = 11.84KK468 pKa = 10.19GDD470 pKa = 3.59WIEE473 pKa = 4.88GILKK477 pKa = 10.37ASEE480 pKa = 4.05FSLEE484 pKa = 4.03HH485 pKa = 6.62KK486 pKa = 10.34EE487 pKa = 3.93YY488 pKa = 11.25VPLFSIDD495 pKa = 3.29ITPEE499 pKa = 3.72CDD501 pKa = 2.78VGNKK505 pKa = 9.06VKK507 pKa = 10.86LLGWLSAIYY516 pKa = 10.23EE517 pKa = 3.96MSIDD521 pKa = 3.73LKK523 pKa = 10.88KK524 pKa = 10.98SHH526 pKa = 6.51VNNNAANEE534 pKa = 4.1DD535 pKa = 3.89LLQEE539 pKa = 3.94IRR541 pKa = 11.84KK542 pKa = 8.22ATSEE546 pKa = 4.26WKK548 pKa = 9.23TKK550 pKa = 10.11AQSAYY555 pKa = 10.63DD556 pKa = 3.92PYY558 pKa = 10.23TGVGILQSYY567 pKa = 6.43MHH569 pKa = 7.03HH570 pKa = 6.9AFAICSSFVEE580 pKa = 4.41EE581 pKa = 3.89RR582 pKa = 11.84ASYY585 pKa = 9.79FSSYY589 pKa = 10.43APPVRR594 pKa = 11.84TMIGNFVSKK603 pKa = 10.83VEE605 pKa = 4.17EE606 pKa = 4.2VSSDD610 pKa = 3.53MIVWGSIIGISSILLSYY627 pKa = 11.42GLFTYY632 pKa = 9.19FFSQEE637 pKa = 3.61QDD639 pKa = 2.78EE640 pKa = 4.46MQLQKK645 pKa = 10.87EE646 pKa = 3.95QDD648 pKa = 3.02IRR650 pKa = 11.84CQVVQGSTMAEE661 pKa = 3.57AVAKK665 pKa = 8.53QTFFMEE671 pKa = 5.4FIKK674 pKa = 10.74KK675 pKa = 10.24DD676 pKa = 3.42GSKK679 pKa = 10.6EE680 pKa = 3.96KK681 pKa = 10.31ATGTVSGHH689 pKa = 6.69FILAPWHH696 pKa = 6.01AAKK699 pKa = 10.58DD700 pKa = 4.0CIRR703 pKa = 11.84ISILSKK709 pKa = 10.88VADD712 pKa = 4.1CRR714 pKa = 11.84LLDD717 pKa = 3.91NAPCTKK723 pKa = 10.24VYY725 pKa = 10.05EE726 pKa = 4.67AYY728 pKa = 10.75SSDD731 pKa = 3.67LVVLKK736 pKa = 10.55IPEE739 pKa = 4.11TLIVPFKK746 pKa = 11.06SLVKK750 pKa = 10.75VFDD753 pKa = 4.19YY754 pKa = 11.01KK755 pKa = 11.03CDD757 pKa = 3.54ALKK760 pKa = 10.86GHH762 pKa = 6.2TLITPYY768 pKa = 11.07GCLPVVKK775 pKa = 9.03RR776 pKa = 11.84TAGCSILNIYY786 pKa = 9.29GKK788 pKa = 9.83NFQLDD793 pKa = 3.77DD794 pKa = 4.51SNSFPYY800 pKa = 9.21TYY802 pKa = 11.33SMDD805 pKa = 3.9GLCGSLLFDD814 pKa = 4.1NATGVIGAHH823 pKa = 5.91IAGNEE828 pKa = 3.94QYY830 pKa = 11.11GRR832 pKa = 11.84ALKK835 pKa = 10.19WNTGVFSEE843 pKa = 5.2VMSLLKK849 pKa = 10.39GDD851 pKa = 4.06RR852 pKa = 11.84FLLEE856 pKa = 5.65LDD858 pKa = 4.07FKK860 pKa = 11.85DD861 pKa = 3.69NGKK864 pKa = 10.24LSSGTQLDD872 pKa = 4.4TNFHH876 pKa = 5.85QSTPKK881 pKa = 8.12VTKK884 pKa = 10.03IEE886 pKa = 3.99PTIFSHH892 pKa = 6.47MFPKK896 pKa = 10.43HH897 pKa = 6.13KK898 pKa = 10.7EE899 pKa = 3.76PVNLSKK905 pKa = 11.12YY906 pKa = 8.58GQHH909 pKa = 5.34TVKK912 pKa = 10.77DD913 pKa = 3.83VAKK916 pKa = 10.5KK917 pKa = 10.37SFSKK921 pKa = 10.73VKK923 pKa = 10.45EE924 pKa = 3.86ITQVEE929 pKa = 4.55LDD931 pKa = 3.74FGLDD935 pKa = 3.57YY936 pKa = 11.09LSTLMQPFSSLSEE949 pKa = 3.79QDD951 pKa = 3.34TVKK954 pKa = 10.95GFDD957 pKa = 3.24KK958 pKa = 10.67VAKK961 pKa = 10.22INMDD965 pKa = 2.85KK966 pKa = 10.08STGIGCEE973 pKa = 3.85RR974 pKa = 11.84DD975 pKa = 2.88RR976 pKa = 11.84AAYY979 pKa = 9.61INFEE983 pKa = 4.08RR984 pKa = 11.84GYY986 pKa = 8.85YY987 pKa = 8.05TQLLRR992 pKa = 11.84KK993 pKa = 8.78EE994 pKa = 4.23IKK996 pKa = 10.4DD997 pKa = 3.69IEE999 pKa = 4.76DD1000 pKa = 3.44NLLKK1004 pKa = 10.77DD1005 pKa = 3.29EE1006 pKa = 4.65HH1007 pKa = 7.45VNLNLWVAKK1016 pKa = 7.78EE1017 pKa = 3.97TLKK1020 pKa = 11.36DD1021 pKa = 3.6EE1022 pKa = 4.3VRR1024 pKa = 11.84SVSKK1028 pKa = 10.64DD1029 pKa = 3.13QEE1031 pKa = 4.03PRR1033 pKa = 11.84SFRR1036 pKa = 11.84VLRR1039 pKa = 11.84FPINVLCKK1047 pKa = 9.8QLTGEE1052 pKa = 4.2MVNNIIANKK1061 pKa = 8.93FSNGIMIGINPYY1073 pKa = 9.43SEE1075 pKa = 4.0WEE1077 pKa = 4.01TLYY1080 pKa = 9.89NTVGYY1085 pKa = 8.86KK1086 pKa = 10.27AKK1088 pKa = 10.75GIIAADD1094 pKa = 3.18IKK1096 pKa = 11.17KK1097 pKa = 10.12FDD1099 pKa = 4.01GGMLPQVQHH1108 pKa = 5.93GVVDD1112 pKa = 4.81TILSFFSGTYY1122 pKa = 9.98KK1123 pKa = 9.71EE1124 pKa = 4.46TEE1126 pKa = 3.95LLKK1129 pKa = 11.15GILHH1133 pKa = 6.14NFINNPVAVNDD1144 pKa = 3.89DD1145 pKa = 4.05VYY1147 pKa = 10.82LTTHH1151 pKa = 6.38SMPSGCYY1158 pKa = 8.15LTAIMNSMVQKK1169 pKa = 10.57VYY1171 pKa = 9.16TAMWYY1176 pKa = 10.14KK1177 pKa = 10.51HH1178 pKa = 6.37VIPDD1182 pKa = 3.64ATVTQYY1188 pKa = 11.34KK1189 pKa = 9.73QDD1191 pKa = 3.27VYY1193 pKa = 11.42DD1194 pKa = 4.46AVYY1197 pKa = 10.93GDD1199 pKa = 5.78DD1200 pKa = 4.19KK1201 pKa = 11.53LCSIKK1206 pKa = 10.89NKK1208 pKa = 10.16EE1209 pKa = 4.63DD1210 pKa = 3.13ILNAITMRR1218 pKa = 11.84DD1219 pKa = 3.59YY1220 pKa = 11.13FQLIGLDD1227 pKa = 4.07LSTADD1232 pKa = 4.35KK1233 pKa = 11.38KK1234 pKa = 11.23EE1235 pKa = 3.54ITQPFDD1241 pKa = 2.5SWEE1244 pKa = 3.99DD1245 pKa = 3.43VNFLKK1250 pKa = 10.61RR1251 pKa = 11.84KK1252 pKa = 9.0FVFHH1256 pKa = 6.8PVLKK1260 pKa = 10.35RR1261 pKa = 11.84IMCPLIDD1268 pKa = 3.51EE1269 pKa = 4.97TFYY1272 pKa = 12.01SMLAWWDD1279 pKa = 3.44TSKK1282 pKa = 11.37DD1283 pKa = 3.76FNDD1286 pKa = 4.15VIDD1289 pKa = 4.22GKK1291 pKa = 10.8LRR1293 pKa = 11.84SFTYY1297 pKa = 10.22EE1298 pKa = 3.51SYY1300 pKa = 10.98LRR1302 pKa = 11.84EE1303 pKa = 4.53DD1304 pKa = 3.31NDD1306 pKa = 4.32SNRR1309 pKa = 11.84EE1310 pKa = 3.88KK1311 pKa = 11.12LKK1313 pKa = 10.39MYY1315 pKa = 10.34LEE1317 pKa = 4.71NVAGMTWIMPSNMEE1331 pKa = 4.44MYY1333 pKa = 10.49RR1334 pKa = 11.84QFLKK1338 pKa = 11.3NEE1340 pKa = 3.71VDD1342 pKa = 3.57YY1343 pKa = 8.4TTYY1346 pKa = 11.43AA1347 pKa = 3.21

Molecular weight:
153.04 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2174

827

1347

1087.0

121.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.474 ± 0.535

1.472 ± 0.289

6.256 ± 0.328

3.864 ± 1.035

5.06 ± 0.335

5.52 ± 0.301

2.254 ± 0.233

6.624 ± 0.085

6.21 ± 2.032

9.108 ± 0.576

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.174 ± 0.017

5.244 ± 0.252

4.416 ± 1.279

3.542 ± 0.187

3.496 ± 0.145

8.832 ± 0.62

6.716 ± 0.932

7.82 ± 0.3

1.012 ± 0.233

3.91 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski