Bradymonas sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Bradymonadales; Bradymonadaceae; Bradymonas

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4FJB3|A0A2Z4FJB3_9DELT Uncharacterized protein OS=Bradymonas sediminis OX=1548548 GN=DN745_06080 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.83SEE5 pKa = 4.71CPTDD9 pKa = 4.38RR10 pKa = 11.84VCGDD14 pKa = 3.57EE15 pKa = 4.61GVCVAPDD22 pKa = 4.05DD23 pKa = 4.57EE24 pKa = 6.03PDD26 pKa = 4.7AGDD29 pKa = 4.13VDD31 pKa = 5.47NNDD34 pKa = 3.73PDD36 pKa = 5.78AGDD39 pKa = 3.81VDD41 pKa = 5.26NNDD44 pKa = 3.89PDD46 pKa = 4.31GGSDD50 pKa = 3.75TLCPAPTITPAGAQNDD66 pKa = 4.09SSSASALPGSTIRR79 pKa = 11.84LDD81 pKa = 3.46GRR83 pKa = 11.84RR84 pKa = 11.84DD85 pKa = 3.32DD86 pKa = 4.71DD87 pKa = 5.22AVVTYY92 pKa = 7.45QWRR95 pKa = 11.84FVDD98 pKa = 5.2FPYY101 pKa = 10.75SEE103 pKa = 4.83TIAEE107 pKa = 4.46LEE109 pKa = 4.32PKK111 pKa = 10.31LVAHH115 pKa = 7.17ANGTADD121 pKa = 4.96FKK123 pKa = 11.78AEE125 pKa = 3.86ALGLYY130 pKa = 8.33TVEE133 pKa = 5.92LEE135 pKa = 4.41VKK137 pKa = 9.49DD138 pKa = 4.23ANGASVCPPTTAQVLVEE155 pKa = 4.57TDD157 pKa = 3.22DD158 pKa = 3.64QIYY161 pKa = 10.28VEE163 pKa = 5.15LVWEE167 pKa = 4.7TPGQTDD173 pKa = 3.67KK174 pKa = 11.41DD175 pKa = 3.64ASGPDD180 pKa = 3.37LEE182 pKa = 5.33LHH184 pKa = 5.95YY185 pKa = 10.47KK186 pKa = 9.5HH187 pKa = 7.14PNGTWAQWDD196 pKa = 3.93PPLDD200 pKa = 3.59IFWYY204 pKa = 10.59VPTADD209 pKa = 3.33WGIPGDD215 pKa = 4.09SSDD218 pKa = 4.15DD219 pKa = 3.39PVMRR223 pKa = 11.84RR224 pKa = 11.84FIDD227 pKa = 3.52HH228 pKa = 6.83GPGPEE233 pKa = 4.38AIAHH237 pKa = 6.16KK238 pKa = 10.53KK239 pKa = 10.11ADD241 pKa = 3.85PLVYY245 pKa = 10.34SVGVYY250 pKa = 10.33SYY252 pKa = 11.11DD253 pKa = 3.88DD254 pKa = 3.72KK255 pKa = 11.95DD256 pKa = 4.01FGPSFATVRR265 pKa = 11.84VYY267 pKa = 11.34LDD269 pKa = 3.69GEE271 pKa = 4.39LADD274 pKa = 3.9EE275 pKa = 4.55VPNIEE280 pKa = 4.24MPEE283 pKa = 4.08TNHH286 pKa = 6.09FYY288 pKa = 10.91EE289 pKa = 4.77VLTIDD294 pKa = 4.87AGQQTTEE301 pKa = 4.02YY302 pKa = 10.4LDD304 pKa = 3.27NHH306 pKa = 6.36YY307 pKa = 11.24EE308 pKa = 4.44DD309 pKa = 4.45FLEE312 pKa = 4.21MM313 pKa = 5.02

Molecular weight: 34.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4FLK3|A0A2Z4FLK3_9DELT Uncharacterized protein OS=Bradymonas sediminis OX=1548548 GN=DN745_10170 PE=4 SV=1
MM1 pKa = 7.08TRR3 pKa = 11.84TRR5 pKa = 11.84ALPPTPTTPPTPTPATRR22 pKa = 11.84PTPATRR28 pKa = 11.84PTPTTPPTPTTPPTPTTPRR47 pKa = 11.84AARR50 pKa = 11.84RR51 pKa = 11.84TVLTPSPWKK60 pKa = 8.95TPPSGPPTRR69 pKa = 11.84LRR71 pKa = 11.84RR72 pKa = 11.84PFWAKK77 pKa = 8.87LTARR81 pKa = 11.84AKK83 pKa = 10.08PAAA86 pKa = 4.19

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3697

0

3697

1449336

18

4148

392.0

42.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.932 ± 0.054

1.185 ± 0.029

6.555 ± 0.036

6.998 ± 0.048

3.786 ± 0.023

7.922 ± 0.043

2.003 ± 0.018

4.961 ± 0.028

3.661 ± 0.032

9.706 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.018

3.056 ± 0.024

4.984 ± 0.028

3.608 ± 0.022

6.467 ± 0.039

6.1 ± 0.027

5.107 ± 0.028

6.927 ± 0.031

1.241 ± 0.015

2.56 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski