Rhizobium sp. BK251

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6346 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1RUM2|A0A4R1RUM2_9RHIZ DNA-binding transcriptional LysR family regulator OS=Rhizobium sp. BK251 OX=2512125 GN=EV286_108267 PE=3 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAVVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.32PEE32 pKa = 4.04PVEE35 pKa = 4.04YY36 pKa = 11.08VRR38 pKa = 11.84VCDD41 pKa = 4.69AYY43 pKa = 9.35GTGYY47 pKa = 10.5FYY49 pKa = 10.55IPGTEE54 pKa = 3.7TCLQIGGWVRR64 pKa = 11.84FQVNYY69 pKa = 10.44GRR71 pKa = 11.84DD72 pKa = 3.25IAEE75 pKa = 4.28GSDD78 pKa = 3.45ANSSGRR84 pKa = 11.84GYY86 pKa = 10.87FYY88 pKa = 10.98LQTKK92 pKa = 10.32NDD94 pKa = 3.79TEE96 pKa = 4.44YY97 pKa = 9.76GTLTGFIAVEE107 pKa = 4.29GDD109 pKa = 3.48VSGQDD114 pKa = 3.29ASVNPYY120 pKa = 9.82FDD122 pKa = 3.28EE123 pKa = 6.48AYY125 pKa = 10.38LQLGGFKK132 pKa = 10.65AGWFYY137 pKa = 11.62NWWDD141 pKa = 3.19KK142 pKa = 11.36GINGEE147 pKa = 4.42QDD149 pKa = 3.37TLNSGGNEE157 pKa = 3.94WVSVSYY163 pKa = 9.79TYY165 pKa = 9.66EE166 pKa = 3.7TDD168 pKa = 3.05NFSIGGAVDD177 pKa = 3.33EE178 pKa = 5.0LDD180 pKa = 3.7RR181 pKa = 11.84DD182 pKa = 3.95GLSTVFAPGDD192 pKa = 4.23PINDD196 pKa = 3.72SNVVVEE202 pKa = 5.34DD203 pKa = 3.65SFDD206 pKa = 4.42GIDD209 pKa = 3.35YY210 pKa = 10.73GFEE213 pKa = 4.07GLITATFGGVSFDD226 pKa = 4.98LLGSYY231 pKa = 11.35DD232 pKa = 3.6NFNEE236 pKa = 3.88EE237 pKa = 3.85GAIRR241 pKa = 11.84ALMSAEE247 pKa = 4.52LGPGTLQAALMWASGQNVYY266 pKa = 10.43FDD268 pKa = 3.63EE269 pKa = 5.05SEE271 pKa = 3.83WSLAASYY278 pKa = 10.42AYY280 pKa = 9.5KK281 pKa = 11.02VNDD284 pKa = 4.36KK285 pKa = 9.84LTITPGGQYY294 pKa = 10.13FWQSSLDD301 pKa = 3.67DD302 pKa = 4.83DD303 pKa = 5.19GSFEE307 pKa = 6.38GGDD310 pKa = 3.16TWDD313 pKa = 3.77AGVTVDD319 pKa = 4.18YY320 pKa = 10.74RR321 pKa = 11.84ITEE324 pKa = 4.21GLTSKK329 pKa = 10.65ISVQYY334 pKa = 11.15NDD336 pKa = 5.45LGDD339 pKa = 4.14SDD341 pKa = 5.46DD342 pKa = 5.16DD343 pKa = 4.16SVSGFFRR350 pKa = 11.84LQRR353 pKa = 11.84TFF355 pKa = 3.23

Molecular weight: 38.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1RGA3|A0A4R1RGA3_9RHIZ Response regulator receiver domain-containing protein OS=Rhizobium sp. BK251 OX=2512125 GN=EV286_11453 PE=4 SV=1
MM1 pKa = 7.19SRR3 pKa = 11.84IHH5 pKa = 6.66AATRR9 pKa = 11.84LKK11 pKa = 10.02STLRR15 pKa = 11.84RR16 pKa = 11.84CLPSGGPCVGRR27 pKa = 11.84CRR29 pKa = 11.84GIRR32 pKa = 11.84DD33 pKa = 3.29ILVFPVMSAAEE44 pKa = 4.49GEE46 pKa = 4.46VSSPIMAGMSFRR58 pKa = 11.84MRR60 pKa = 11.84LHH62 pKa = 6.55GEE64 pKa = 3.66NKK66 pKa = 9.67GRR68 pKa = 11.84STFIRR73 pKa = 11.84SASPRR78 pKa = 11.84RR79 pKa = 11.84SFGRR83 pKa = 11.84KK84 pKa = 6.98QRR86 pKa = 11.84TGGIAFLSIFGRR98 pKa = 11.84ATLNLPDD105 pKa = 4.79RR106 pKa = 11.84GCGPLRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84DD115 pKa = 3.64GEE117 pKa = 4.47PIFAEE122 pKa = 4.41PWHH125 pKa = 5.76AQIMAIADD133 pKa = 4.04LLIGSGPIPQSQWTQTLGKK152 pKa = 8.75EE153 pKa = 3.85LRR155 pKa = 11.84LARR158 pKa = 11.84LADD161 pKa = 3.84SADD164 pKa = 3.72DD165 pKa = 3.34TSTYY169 pKa = 8.29YY170 pKa = 10.42TAVLSALEE178 pKa = 4.72RR179 pKa = 11.84ILDD182 pKa = 3.7AGGNVSCVEE191 pKa = 3.76LARR194 pKa = 11.84RR195 pKa = 11.84RR196 pKa = 11.84EE197 pKa = 4.1EE198 pKa = 3.38WRR200 pKa = 11.84QAYY203 pKa = 9.72LHH205 pKa = 5.7THH207 pKa = 6.52HH208 pKa = 6.62GQPVEE213 pKa = 4.08LEE215 pKa = 3.92SRR217 pKa = 11.84FAKK220 pKa = 10.24TSDD223 pKa = 2.94

Molecular weight: 24.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6346

0

6346

1948081

29

2834

307.0

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.847 ± 0.04

0.79 ± 0.008

5.654 ± 0.027

5.844 ± 0.028

3.925 ± 0.018

8.348 ± 0.033

2.039 ± 0.014

5.653 ± 0.024

3.54 ± 0.025

10.002 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.014

2.847 ± 0.018

4.929 ± 0.022

3.084 ± 0.018

6.806 ± 0.027

5.863 ± 0.023

5.269 ± 0.02

7.391 ± 0.026

1.31 ± 0.012

2.328 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski