Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium fujikuroi species complex; Fusarium fujikuroi

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0DWK4|S0DWK4_GIBF5 Uncharacterized protein OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OX=1279085 GN=FFUJ_13049 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.43SVLTFAGLAFASFAAAGPCRR22 pKa = 11.84PRR24 pKa = 11.84TTTTAAAVSSSTEE37 pKa = 3.94IASSTATEE45 pKa = 4.03TAAVSTSTGYY55 pKa = 10.21SVPVVSEE62 pKa = 4.14SSTTLATVIVSEE74 pKa = 4.53TSATEE79 pKa = 3.85TSAGEE84 pKa = 4.03TSATGTTTAEE94 pKa = 3.87TSAAGTTTADD104 pKa = 3.26TSAVDD109 pKa = 3.8TTTTAADD116 pKa = 3.5TTTTVEE122 pKa = 4.24GTTTTVADD130 pKa = 3.79TTTAAEE136 pKa = 4.32DD137 pKa = 3.96TTTTAADD144 pKa = 3.65TTTTAEE150 pKa = 4.14GTTTAEE156 pKa = 4.16GTTTEE161 pKa = 4.3GTTTAAEE168 pKa = 4.3TTTTAEE174 pKa = 4.3GTTTTAEE181 pKa = 4.13DD182 pKa = 3.65TTTAEE187 pKa = 4.15TTTAEE192 pKa = 4.42GTTTAADD199 pKa = 3.66TTTTAEE205 pKa = 4.33GTTTTAEE212 pKa = 4.18GTTTADD218 pKa = 3.2ATTSTSAEE226 pKa = 3.9PTADD230 pKa = 3.44QSCDD234 pKa = 3.45NAGLEE239 pKa = 4.1YY240 pKa = 10.43AIYY243 pKa = 10.63KK244 pKa = 7.96HH245 pKa = 5.67TFYY248 pKa = 11.24NSDD251 pKa = 3.58PPHH254 pKa = 6.65FSSFDD259 pKa = 3.19PTFFHH264 pKa = 6.67TATPTYY270 pKa = 9.93QGEE273 pKa = 4.39TTRR276 pKa = 11.84IGIPPGTASDD286 pKa = 3.67TSFAIYY292 pKa = 10.25DD293 pKa = 3.97GSPSQLWQYY302 pKa = 10.82KK303 pKa = 9.4AVNHH307 pKa = 6.28RR308 pKa = 11.84AFLYY312 pKa = 10.7APEE315 pKa = 4.48TGDD318 pKa = 3.43YY319 pKa = 10.1TVTIPNSDD327 pKa = 4.63EE328 pKa = 3.6ITLIWFGEE336 pKa = 4.16KK337 pKa = 10.04AIEE340 pKa = 4.12GWTRR344 pKa = 11.84ANADD348 pKa = 4.03LEE350 pKa = 4.0QDD352 pKa = 4.0YY353 pKa = 10.87PGGTSKK359 pKa = 9.61TFTIHH364 pKa = 5.93LTAGTYY370 pKa = 9.46TPFRR374 pKa = 11.84LLWANAQGDD383 pKa = 4.42LNFIAEE389 pKa = 4.19VQAPDD394 pKa = 3.22GKK396 pKa = 11.2VIVNGDD402 pKa = 3.32GSDD405 pKa = 2.8NKK407 pKa = 10.48YY408 pKa = 10.16FVRR411 pKa = 11.84FACDD415 pKa = 2.79EE416 pKa = 4.32STPSFPDD423 pKa = 3.52FGYY426 pKa = 11.13GGG428 pKa = 3.49

Molecular weight: 43.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0E214|S0E214_GIBF5 Related to transcriptional coactivator HFI1 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OX=1279085 GN=FFUJ_07552 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APVHH26 pKa = 4.79SHH28 pKa = 5.43RR29 pKa = 11.84HH30 pKa = 4.01TTTTTTTTTKK40 pKa = 9.5PRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GMFGGGASRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 5.59ATATAPVHH64 pKa = 4.93HH65 pKa = 6.66HH66 pKa = 5.18QRR68 pKa = 11.84RR69 pKa = 11.84PSMKK73 pKa = 10.02DD74 pKa = 2.95KK75 pKa = 11.41VSGALLKK82 pKa = 11.04LKK84 pKa = 10.68GSLTRR89 pKa = 11.84RR90 pKa = 11.84PGVKK94 pKa = 9.89AAGTRR99 pKa = 11.84RR100 pKa = 11.84MRR102 pKa = 11.84GTDD105 pKa = 3.02GRR107 pKa = 11.84GARR110 pKa = 11.84HH111 pKa = 5.87HH112 pKa = 7.23RR113 pKa = 11.84YY114 pKa = 9.44

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14792

0

14792

7170111

23

7581

484.7

53.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.3 ± 0.019

1.331 ± 0.008

5.862 ± 0.016

6.207 ± 0.019

3.803 ± 0.014

6.748 ± 0.019

2.4 ± 0.008

5.16 ± 0.013

5.039 ± 0.019

8.866 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.008

3.776 ± 0.009

5.86 ± 0.023

4.004 ± 0.014

5.829 ± 0.016

8.121 ± 0.025

6.009 ± 0.026

6.092 ± 0.013

1.552 ± 0.007

2.818 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski