[Clostridium] polysaccharolyticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9Z6V7|A0A1H9Z6V7_9FIRM Uracil permease OS=[Clostridium] polysaccharolyticum OX=29364 GN=SAMN04487772_10329 PE=3 SV=1
MM1 pKa = 7.67DD2 pKa = 5.14LEE4 pKa = 4.21NRR6 pKa = 11.84VLCASSAYY14 pKa = 8.91EE15 pKa = 3.49EE16 pKa = 4.35KK17 pKa = 10.94YY18 pKa = 10.59YY19 pKa = 11.01FNEE22 pKa = 4.88FYY24 pKa = 10.97SGIPTAIQEE33 pKa = 4.53EE34 pKa = 4.44LQIMCVTFTSEE45 pKa = 3.7VGGILSLEE53 pKa = 3.99YY54 pKa = 10.72DD55 pKa = 3.62EE56 pKa = 5.6EE57 pKa = 6.18GNLSLSVAADD67 pKa = 3.84EE68 pKa = 5.64GDD70 pKa = 3.6LLYY73 pKa = 11.14DD74 pKa = 4.65EE75 pKa = 5.59IGSGLKK81 pKa = 9.82IKK83 pKa = 10.2EE84 pKa = 3.72MQRR87 pKa = 11.84NKK89 pKa = 10.86NEE91 pKa = 3.88LFEE94 pKa = 5.03ALEE97 pKa = 4.22TYY99 pKa = 10.78YY100 pKa = 11.13KK101 pKa = 10.94VFILKK106 pKa = 9.65QDD108 pKa = 3.72LSSLIEE114 pKa = 3.92EE115 pKa = 4.72DD116 pKa = 3.33

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0F3P5|A0A1I0F3P5_9FIRM Multiple sugar transport system permease protein OS=[Clostridium] polysaccharolyticum OX=29364 GN=SAMN04487772_12730 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSSNGRR28 pKa = 11.84KK29 pKa = 8.71VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.14QLSAA44 pKa = 3.9

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3018

0

3018

1022225

16

3480

338.7

38.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.677 ± 0.048

1.494 ± 0.015

5.397 ± 0.031

7.712 ± 0.065

4.242 ± 0.031

6.632 ± 0.041

1.664 ± 0.018

7.787 ± 0.042

8.02 ± 0.044

8.651 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.908 ± 0.022

4.992 ± 0.033

2.948 ± 0.022

3.438 ± 0.029

3.788 ± 0.03

6.164 ± 0.04

5.25 ± 0.04

6.918 ± 0.036

0.883 ± 0.017

4.435 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski