Hubei narna-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KIL8|A0A1L3KIL8_9VIRU Uncharacterized protein OS=Hubei narna-like virus 21 OX=1922952 PE=4 SV=1
MM1 pKa = 7.2GRR3 pKa = 11.84EE4 pKa = 3.97EE5 pKa = 3.88TVKK8 pKa = 10.39RR9 pKa = 11.84VKK11 pKa = 10.36QYY13 pKa = 11.64AEE15 pKa = 3.8YY16 pKa = 10.59CRR18 pKa = 11.84ARR20 pKa = 11.84SIDD23 pKa = 3.28ARR25 pKa = 11.84QAEE28 pKa = 4.53AGPPRR33 pKa = 11.84GFPFHH38 pKa = 6.95ALEE41 pKa = 4.76GGSFKK46 pKa = 11.1ALLRR50 pKa = 11.84CPVQGPAALAQVARR64 pKa = 11.84LGRR67 pKa = 11.84SMPLPTHH74 pKa = 6.13KK75 pKa = 10.49QQGRR79 pKa = 11.84ALIEE83 pKa = 4.14HH84 pKa = 6.71CNEE87 pKa = 3.73LTAGPHH93 pKa = 5.17EE94 pKa = 5.03EE95 pKa = 4.37IPVEE99 pKa = 3.89LLDD102 pKa = 4.84AFYY105 pKa = 11.09YY106 pKa = 7.15WTRR109 pKa = 11.84TWGAGKK115 pKa = 9.99GAALRR120 pKa = 11.84DD121 pKa = 3.65EE122 pKa = 4.46VTVNFSSAAAEE133 pKa = 4.28SVSRR137 pKa = 11.84ARR139 pKa = 11.84GGQRR143 pKa = 11.84EE144 pKa = 4.27EE145 pKa = 4.35LRR147 pKa = 11.84LLAAPHH153 pKa = 6.26FEE155 pKa = 5.0AIRR158 pKa = 11.84EE159 pKa = 4.06ALAIDD164 pKa = 5.25DD165 pKa = 3.19IDD167 pKa = 3.93FRR169 pKa = 11.84VEE171 pKa = 3.59EE172 pKa = 4.34GDD174 pKa = 3.52YY175 pKa = 11.25AGIYY179 pKa = 9.96SEE181 pKa = 5.16EE182 pKa = 4.84DD183 pKa = 3.16DD184 pKa = 5.91LEE186 pKa = 6.46IITQAAIEE194 pKa = 4.21RR195 pKa = 11.84VRR197 pKa = 11.84ADD199 pKa = 2.57IRR201 pKa = 11.84AGRR204 pKa = 11.84RR205 pKa = 11.84PDD207 pKa = 2.92VRR209 pKa = 11.84AAVVPEE215 pKa = 4.7LGAKK219 pKa = 9.58SRR221 pKa = 11.84IVTAGEE227 pKa = 3.83AHH229 pKa = 6.23WVVIGDD235 pKa = 5.38AIRR238 pKa = 11.84KK239 pKa = 7.73CLWPALEE246 pKa = 4.35AEE248 pKa = 4.25PRR250 pKa = 11.84IDD252 pKa = 3.27LSGRR256 pKa = 11.84RR257 pKa = 11.84DD258 pKa = 3.58VAGTAAHH265 pKa = 6.36LHH267 pKa = 6.55DD268 pKa = 5.03EE269 pKa = 4.37VAKK272 pKa = 10.78ALTGAEE278 pKa = 3.67AVEE281 pKa = 5.55FYY283 pKa = 11.1SADD286 pKa = 3.28LTAATDD292 pKa = 4.14LMPHH296 pKa = 7.0DD297 pKa = 6.14LIQAIWRR304 pKa = 11.84GLMEE308 pKa = 4.63GLGVSDD314 pKa = 5.23DD315 pKa = 4.87DD316 pKa = 5.53LMFTAGLALLGPVNLHH332 pKa = 6.81HH333 pKa = 7.73PDD335 pKa = 4.22LDD337 pKa = 4.18DD338 pKa = 4.92LDD340 pKa = 5.53RR341 pKa = 11.84PGKK344 pKa = 8.52YY345 pKa = 10.16AYY347 pKa = 10.14GVTHH351 pKa = 7.35KK352 pKa = 10.34GCMMGFPLSWYY363 pKa = 9.76ILNLYY368 pKa = 9.93NLACADD374 pKa = 3.99LALSAEE380 pKa = 4.29RR381 pKa = 11.84GRR383 pKa = 11.84FLAEE387 pKa = 3.44VAPRR391 pKa = 11.84LVGAAPAVVRR401 pKa = 11.84GDD403 pKa = 4.28DD404 pKa = 3.66LCSAHH409 pKa = 6.23TPAEE413 pKa = 3.78ADD415 pKa = 3.25RR416 pKa = 11.84YY417 pKa = 9.66EE418 pKa = 4.58RR419 pKa = 11.84IIRR422 pKa = 11.84ATGGRR427 pKa = 11.84ANLSKK432 pKa = 10.56SYY434 pKa = 10.5RR435 pKa = 11.84SRR437 pKa = 11.84KK438 pKa = 9.69GFILAEE444 pKa = 4.03RR445 pKa = 11.84TFLVTTARR453 pKa = 11.84TKK455 pKa = 10.8CSAARR460 pKa = 11.84SRR462 pKa = 11.84LGTVVVRR469 pKa = 11.84PGVHH473 pKa = 6.08PRR475 pKa = 11.84APLLTRR481 pKa = 11.84APDD484 pKa = 3.58AFEE487 pKa = 4.83SGALGYY493 pKa = 10.53RR494 pKa = 11.84SIDD497 pKa = 3.09PRR499 pKa = 11.84SRR501 pKa = 11.84RR502 pKa = 11.84RR503 pKa = 11.84GAATVVGLTMLSDD516 pKa = 3.65VPVRR520 pKa = 11.84HH521 pKa = 6.4LMPSPDD527 pKa = 3.94SEE529 pKa = 4.24GLPAYY534 pKa = 8.35VTLPPAASAVVDD546 pKa = 4.54EE547 pKa = 5.01IKK549 pKa = 10.52DD550 pKa = 3.37IKK552 pKa = 10.46RR553 pKa = 11.84RR554 pKa = 11.84EE555 pKa = 4.24AICYY559 pKa = 9.76GILSVNAPLVRR570 pKa = 11.84QFNRR574 pKa = 11.84FKK576 pKa = 10.92IPIFYY581 pKa = 9.95PRR583 pKa = 11.84EE584 pKa = 3.71LGGAGFPHH592 pKa = 7.06PKK594 pKa = 10.38GFGSAIRR601 pKa = 11.84SAGGMGHH608 pKa = 7.38LRR610 pKa = 11.84ATLAMTTFTRR620 pKa = 11.84RR621 pKa = 11.84ARR623 pKa = 11.84QAYY626 pKa = 9.46QLDD629 pKa = 4.18RR630 pKa = 11.84DD631 pKa = 3.62PWICDD636 pKa = 3.05RR637 pKa = 11.84QVEE640 pKa = 4.64FEE642 pKa = 4.12RR643 pKa = 11.84SRR645 pKa = 11.84AQTGLIAEE653 pKa = 4.42EE654 pKa = 3.82RR655 pKa = 11.84RR656 pKa = 11.84AAEE659 pKa = 4.12SGQLGLATSVPLEE672 pKa = 4.26DD673 pKa = 4.27AVAARR678 pKa = 11.84AAEE681 pKa = 4.24GQLWAEE687 pKa = 4.13VMLPQSRR694 pKa = 11.84EE695 pKa = 3.72LASARR700 pKa = 11.84RR701 pKa = 11.84GRR703 pKa = 11.84RR704 pKa = 11.84GPRR707 pKa = 11.84LSSVRR712 pKa = 11.84KK713 pKa = 9.52GLSDD717 pKa = 3.96VFNALAKK724 pKa = 10.03VHH726 pKa = 6.71AKK728 pKa = 10.08FDD730 pKa = 4.19PKK732 pKa = 10.62FLANPRR738 pKa = 11.84IDD740 pKa = 3.38KK741 pKa = 7.32EE742 pKa = 4.25TWMDD746 pKa = 3.62RR747 pKa = 11.84VAMAKK752 pKa = 10.27GSSTVYY758 pKa = 9.97RR759 pKa = 11.84VVRR762 pKa = 11.84PSGRR766 pKa = 11.84RR767 pKa = 11.84VVMHH771 pKa = 7.19GIRR774 pKa = 11.84DD775 pKa = 3.56THH777 pKa = 7.54RR778 pKa = 11.84EE779 pKa = 3.47IRR781 pKa = 11.84AEE783 pKa = 3.9RR784 pKa = 11.84EE785 pKa = 3.78MLKK788 pKa = 10.82GGWTFGGQRR797 pKa = 11.84DD798 pKa = 3.88LRR800 pKa = 11.84QNTAAGLEE808 pKa = 4.25GGEE811 pKa = 4.17IAVPVPTQRR820 pKa = 11.84AGATLWDD827 pKa = 4.06VLSVSCKK834 pKa = 9.19RR835 pKa = 11.84ARR837 pKa = 11.84KK838 pKa = 8.94RR839 pKa = 11.84KK840 pKa = 9.47KK841 pKa = 10.33GNPRR845 pKa = 11.84LL846 pKa = 3.73

Molecular weight:
92.67 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KIL8|A0A1L3KIL8_9VIRU Uncharacterized protein OS=Hubei narna-like virus 21 OX=1922952 PE=4 SV=1
MM1 pKa = 7.64LLQAGVAFLPFPSALTAHH19 pKa = 6.37TEE21 pKa = 4.08YY22 pKa = 10.49IPEE25 pKa = 4.52CSSSSLCRR33 pKa = 11.84YY34 pKa = 10.14RR35 pKa = 11.84NGNLPSFQTCRR46 pKa = 11.84GVLPQVALAPKK57 pKa = 10.08CPPTLQHH64 pKa = 6.71LSLRR68 pKa = 11.84TDD70 pKa = 4.2FPVRR74 pKa = 11.84IPNSMHH80 pKa = 6.59NDD82 pKa = 2.98APAARR87 pKa = 11.84SNNPINGTRR96 pKa = 11.84TLRR99 pKa = 11.84HH100 pKa = 6.31CNAVHH105 pKa = 7.08PGLLVNPRR113 pKa = 11.84IGKK116 pKa = 9.15KK117 pKa = 10.12LRR119 pKa = 11.84IEE121 pKa = 4.03LRR123 pKa = 11.84VDD125 pKa = 4.13FSEE128 pKa = 4.69GVEE131 pKa = 4.27HH132 pKa = 6.76VRR134 pKa = 11.84EE135 pKa = 4.19PLPHH139 pKa = 6.35RR140 pKa = 11.84RR141 pKa = 11.84EE142 pKa = 3.98ARR144 pKa = 11.84TATPAPRR151 pKa = 11.84GGQLAGLRR159 pKa = 11.84QHH161 pKa = 6.58NLGPEE166 pKa = 3.98LSLRR170 pKa = 11.84RR171 pKa = 11.84SRR173 pKa = 11.84GHH175 pKa = 6.66RR176 pKa = 11.84VLQRR180 pKa = 11.84HH181 pKa = 5.18RR182 pKa = 11.84RR183 pKa = 11.84GEE185 pKa = 4.19SEE187 pKa = 4.27LSAFSGAALFRR198 pKa = 11.84DD199 pKa = 3.94QSGLRR204 pKa = 11.84AATFKK209 pKa = 11.2FNLTVAYY216 pKa = 9.05PRR218 pKa = 11.84IAIEE222 pKa = 3.92LVRR225 pKa = 11.84LSRR228 pKa = 11.84SAGEE232 pKa = 3.89GRR234 pKa = 11.84HH235 pKa = 5.45CKK237 pKa = 9.85RR238 pKa = 11.84RR239 pKa = 11.84SQMTHH244 pKa = 4.86TSRR247 pKa = 11.84GADD250 pKa = 3.27GASEE254 pKa = 4.37SLRR257 pKa = 11.84MRR259 pKa = 11.84KK260 pKa = 9.3PGSPEE265 pKa = 3.3LSRR268 pKa = 11.84IEE270 pKa = 4.3DD271 pKa = 3.56RR272 pKa = 11.84NLEE275 pKa = 4.64PIEE278 pKa = 4.91LPHH281 pKa = 6.01QRR283 pKa = 11.84SVYY286 pKa = 7.05TQNPITDD293 pKa = 4.47RR294 pKa = 11.84LPPLYY299 pKa = 10.18ILDD302 pKa = 4.15FVHH305 pKa = 7.18HH306 pKa = 6.02STSCRR311 pKa = 11.84RR312 pKa = 11.84KK313 pKa = 9.84RR314 pKa = 11.84DD315 pKa = 3.28IRR317 pKa = 11.84WQPFRR322 pKa = 11.84VWRR325 pKa = 11.84RR326 pKa = 11.84HH327 pKa = 4.43QMPDD331 pKa = 2.9RR332 pKa = 11.84NVAQHH337 pKa = 6.71RR338 pKa = 11.84EE339 pKa = 3.69AHH341 pKa = 5.81NRR343 pKa = 11.84GCAPPTAARR352 pKa = 11.84IDD354 pKa = 3.55RR355 pKa = 11.84TVPKK359 pKa = 8.76GTRR362 pKa = 11.84FEE364 pKa = 4.6RR365 pKa = 11.84IRR367 pKa = 11.84RR368 pKa = 11.84ARR370 pKa = 11.84QQGSPRR376 pKa = 11.84MNPRR380 pKa = 11.84SHH382 pKa = 7.72DD383 pKa = 3.86DD384 pKa = 3.55CPQTGSCRR392 pKa = 11.84GTFGPRR398 pKa = 11.84CGHH401 pKa = 5.7QEE403 pKa = 4.0GPLSKK408 pKa = 10.92DD409 pKa = 3.26EE410 pKa = 5.16AFTTPVRR417 pKa = 11.84LAEE420 pKa = 4.24VRR422 pKa = 11.84SSPCCSDD429 pKa = 3.9DD430 pKa = 3.46PLIAVRR436 pKa = 11.84LCRR439 pKa = 11.84RR440 pKa = 11.84VRR442 pKa = 11.84RR443 pKa = 11.84AQIISPDD450 pKa = 3.37NCRR453 pKa = 11.84SSAHH457 pKa = 5.27QPRR460 pKa = 11.84SYY462 pKa = 10.89LRR464 pKa = 11.84EE465 pKa = 3.52KK466 pKa = 10.58AAALGRR472 pKa = 11.84QSQVSTGKK480 pKa = 9.94VVEE483 pKa = 4.42VKK485 pKa = 10.57NIPRR489 pKa = 11.84KK490 pKa = 10.0RR491 pKa = 11.84EE492 pKa = 3.63AHH494 pKa = 6.06HH495 pKa = 6.2AAFMGNPIGIFTRR508 pKa = 11.84TIQIVEE514 pKa = 3.77VRR516 pKa = 11.84MMQVHH521 pKa = 6.76RR522 pKa = 11.84SEE524 pKa = 4.04QSKK527 pKa = 9.97PSCEE531 pKa = 4.08HH532 pKa = 5.02QVVVGNAQTFHH543 pKa = 6.64EE544 pKa = 4.75PSPNGLNKK552 pKa = 9.64VMRR555 pKa = 11.84HH556 pKa = 4.48EE557 pKa = 4.04VRR559 pKa = 11.84GRR561 pKa = 11.84GQVCRR566 pKa = 11.84VEE568 pKa = 4.58LDD570 pKa = 3.35SFRR573 pKa = 11.84AGQGFGNLIVKK584 pKa = 8.19MSRR587 pKa = 11.84RR588 pKa = 11.84SSDD591 pKa = 2.41IATSRR596 pKa = 11.84EE597 pKa = 3.87VNPWLRR603 pKa = 11.84LQSGPQTLPDD613 pKa = 4.08RR614 pKa = 11.84VTDD617 pKa = 3.61DD618 pKa = 4.08HH619 pKa = 6.83PVCFTRR625 pKa = 11.84SDD627 pKa = 3.6NAGLCSQLRR636 pKa = 11.84NHH638 pKa = 7.45RR639 pKa = 11.84GPHH642 pKa = 5.68IGATACADD650 pKa = 3.14IRR652 pKa = 11.84ADD654 pKa = 3.74ALDD657 pKa = 3.74GGLRR661 pKa = 11.84YY662 pKa = 10.04DD663 pKa = 4.21FKK665 pKa = 11.14IILLRR670 pKa = 11.84VNAGVVSFFDD680 pKa = 3.61PKK682 pKa = 10.95VDD684 pKa = 3.56VVDD687 pKa = 4.06RR688 pKa = 11.84QGLSDD693 pKa = 3.48RR694 pKa = 11.84FKK696 pKa = 11.17VRR698 pKa = 11.84RR699 pKa = 11.84CKK701 pKa = 10.4QPEE704 pKa = 4.02FLALTTPGPAHH715 pKa = 7.04RR716 pKa = 11.84FGSRR720 pKa = 11.84GRR722 pKa = 11.84EE723 pKa = 4.22VNCHH727 pKa = 7.04LIAEE731 pKa = 4.71RR732 pKa = 11.84CSLTCPPCSRR742 pKa = 11.84PVIEE746 pKa = 4.83RR747 pKa = 11.84VEE749 pKa = 4.02EE750 pKa = 3.91FHH752 pKa = 7.38WNFLVGSGGEE762 pKa = 4.27FIAVLDD768 pKa = 3.87EE769 pKa = 4.34CAPLLLMCRR778 pKa = 11.84QRR780 pKa = 11.84HH781 pKa = 5.59RR782 pKa = 11.84SPQSGNLSEE791 pKa = 5.46GRR793 pKa = 11.84GALNRR798 pKa = 11.84TPKK801 pKa = 10.45EE802 pKa = 3.98SLKK805 pKa = 10.71RR806 pKa = 11.84PSLEE810 pKa = 3.8CVEE813 pKa = 4.86RR814 pKa = 11.84EE815 pKa = 4.06TPRR818 pKa = 11.84RR819 pKa = 11.84TRR821 pKa = 11.84LGLPGVDD828 pKa = 3.07RR829 pKa = 11.84ARR831 pKa = 11.84ATVLCVLFNTLDD843 pKa = 3.85GFFSAHH849 pKa = 6.9KK850 pKa = 10.52GPGLFQPFAEE860 pKa = 4.56LQEE863 pKa = 4.61SLCSSPMARR872 pKa = 11.84IRR874 pKa = 11.84QFPSAPLQGWEE885 pKa = 4.12KK886 pKa = 10.77RR887 pKa = 11.84EE888 pKa = 3.7YY889 pKa = 10.09DD890 pKa = 3.81QPEE893 pKa = 4.37TVPNTVDD900 pKa = 3.21CVEE903 pKa = 4.49IFDD906 pKa = 5.21AGWAQICRR914 pKa = 11.84LLGAFRR920 pKa = 11.84GQSRR924 pKa = 11.84GGGRR928 pKa = 11.84TPLLLVGPLRR938 pKa = 11.84GDD940 pKa = 3.24QNRR943 pKa = 11.84PVSKK947 pKa = 10.8SRR949 pKa = 11.84WW950 pKa = 3.45

Molecular weight:
106.61 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1796

846

950

898.0

99.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.134 ± 2.463

2.394 ± 0.753

4.677 ± 0.523

5.902 ± 0.412

3.452 ± 0.342

7.962 ± 0.459

3.062 ± 0.481

3.842 ± 0.203

2.951 ± 0.247

8.964 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.615 ± 0.19

2.561 ± 0.787

6.849 ± 0.809

3.563 ± 0.826

12.194 ± 0.664

6.347 ± 0.87

4.51 ± 0.013

6.514 ± 0.154

1.002 ± 0.205

1.503 ± 0.511

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski