Kryptolebias marmoratus (Mangrove killifish) (Rivulus marmoratus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Ne

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q2ZA23|A0A3Q2ZA23_KRYMA Transcriptional regulator OS=Kryptolebias marmoratus OX=37003 PE=4 SV=1
MM1 pKa = 7.11KK2 pKa = 9.59TLALLCVCALLSVCWSMGAVEE23 pKa = 4.69PEE25 pKa = 4.42VVVDD29 pKa = 4.48PAADD33 pKa = 3.69SAVDD37 pKa = 3.41PAAAATHH44 pKa = 6.27SSSSSSSEE52 pKa = 3.8SDD54 pKa = 3.34SASAAASDD62 pKa = 4.25SSSSDD67 pKa = 2.82SSASDD72 pKa = 3.26SSSSSDD78 pKa = 3.72SSASDD83 pKa = 3.6SNSEE87 pKa = 3.98SSEE90 pKa = 3.87SSEE93 pKa = 3.89SSEE96 pKa = 3.95SSEE99 pKa = 4.22SAEE102 pKa = 4.07SSSAEE107 pKa = 4.02SSEE110 pKa = 4.47SASSEE115 pKa = 4.13SDD117 pKa = 3.04SSASDD122 pKa = 3.38SAASSSSSSSSSSSSEE138 pKa = 4.04SASAEE143 pKa = 3.8ASHH146 pKa = 7.63VVMKK150 pKa = 10.59RR151 pKa = 11.84DD152 pKa = 3.39LASVLLRR159 pKa = 11.84RR160 pKa = 11.84GRR162 pKa = 11.84AATGGTLSPLQLEE175 pKa = 4.47SLKK178 pKa = 10.41EE179 pKa = 3.97VCEE182 pKa = 4.28LNVACDD188 pKa = 3.65EE189 pKa = 4.31MADD192 pKa = 3.76TEE194 pKa = 4.87GIVAAYY200 pKa = 6.34TAYY203 pKa = 10.51YY204 pKa = 10.39GPVPFF209 pKa = 5.61

Molecular weight: 20.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q3BHQ3|A0A3Q3BHQ3_KRYMA Transmembrane protein 255B OS=Kryptolebias marmoratus OX=37003 PE=3 SV=1
WW1 pKa = 6.64RR2 pKa = 11.84KK3 pKa = 10.18KK4 pKa = 10.25RR5 pKa = 11.84MRR7 pKa = 11.84LKK9 pKa = 10.19RR10 pKa = 11.84KK11 pKa = 8.23RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.46MRR16 pKa = 11.84QRR18 pKa = 11.84SKK20 pKa = 11.41

Molecular weight: 2.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21873

7813

29686

17386265

17

8587

585.7

65.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.513 ± 0.013

2.25 ± 0.011

5.193 ± 0.01

6.832 ± 0.017

3.753 ± 0.01

6.271 ± 0.016

2.628 ± 0.007

4.36 ± 0.011

5.663 ± 0.014

9.575 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.006

3.895 ± 0.008

5.738 ± 0.017

4.717 ± 0.012

5.656 ± 0.012

8.852 ± 0.016

5.547 ± 0.009

6.34 ± 0.011

1.15 ± 0.005

2.721 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski