Bacillus phage vB_BmeM-Goe8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Goettingenvirus; Bacillus virus vBBmeMGoe8

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KML7|A0A516KML7_9CAUD Uncharacterized protein OS=Bacillus phage vB_BmeM-Goe8 OX=2593638 GN=Goe8_c00540 PE=4 SV=1
MM1 pKa = 7.66FGLTDD6 pKa = 3.77QEE8 pKa = 4.38MEE10 pKa = 4.34SYY12 pKa = 10.89RR13 pKa = 11.84SFAQNFAVEE22 pKa = 4.34EE23 pKa = 4.08LTPQVVTDD31 pKa = 4.3SEE33 pKa = 4.45LLAPVLVKK41 pKa = 10.73GEE43 pKa = 3.98KK44 pKa = 9.03MYY46 pKa = 11.0PDD48 pKa = 3.69MPFVNQLVTDD58 pKa = 4.52LEE60 pKa = 4.75TIVTITTTTMAYY72 pKa = 10.26DD73 pKa = 3.85YY74 pKa = 11.42AHH76 pKa = 6.75LQAEE80 pKa = 4.47SDD82 pKa = 3.67LMEE85 pKa = 5.57AEE87 pKa = 4.78IIEE90 pKa = 4.23KK91 pKa = 10.38LHH93 pKa = 6.04TKK95 pKa = 10.44FEE97 pKa = 4.61TFVFNQFLRR106 pKa = 11.84YY107 pKa = 8.87GVSFSPEE114 pKa = 3.91AITRR118 pKa = 11.84IVSQTILEE126 pKa = 4.34LPYY129 pKa = 9.67MYY131 pKa = 10.57AAIMGDD137 pKa = 3.53EE138 pKa = 4.7EE139 pKa = 4.41FNEE142 pKa = 4.23DD143 pKa = 3.17AFLEE147 pKa = 4.33EE148 pKa = 5.18RR149 pKa = 11.84LQAYY153 pKa = 10.27NDD155 pKa = 3.7YY156 pKa = 10.86LDD158 pKa = 4.61SNFGEE163 pKa = 4.89DD164 pKa = 5.14DD165 pKa = 3.42LTEE168 pKa = 4.14EE169 pKa = 4.25DD170 pKa = 3.97EE171 pKa = 4.83EE172 pKa = 4.96YY173 pKa = 10.7FDD175 pKa = 5.65EE176 pKa = 4.65EE177 pKa = 4.75DD178 pKa = 4.65EE179 pKa = 5.0EE180 pKa = 4.54EE181 pKa = 4.42LDD183 pKa = 3.97HH184 pKa = 7.56

Molecular weight: 21.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KN44|A0A516KN44_9CAUD Uncharacterized protein OS=Bacillus phage vB_BmeM-Goe8 OX=2593638 GN=Goe8_c02330 PE=4 SV=1
MM1 pKa = 6.87NTNSTDD7 pKa = 4.29LINKK11 pKa = 8.77LRR13 pKa = 11.84GVQFCTCGSEE23 pKa = 4.33QKK25 pKa = 10.5KK26 pKa = 9.81VLQNVTTRR34 pKa = 11.84YY35 pKa = 8.53KK36 pKa = 10.43GKK38 pKa = 9.67HH39 pKa = 4.6IRR41 pKa = 11.84IEE43 pKa = 4.3SVPSLVCLRR52 pKa = 11.84NDD54 pKa = 3.18THH56 pKa = 5.7VTHH59 pKa = 6.88TMRR62 pKa = 11.84TRR64 pKa = 11.84RR65 pKa = 11.84NIRR68 pKa = 11.84NLLSIAYY75 pKa = 8.54MNDD78 pKa = 2.7WTHH81 pKa = 5.53ILYY84 pKa = 10.47RR85 pKa = 11.84EE86 pKa = 4.15MGTVV90 pKa = 3.01

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

46406

32

1279

204.4

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.717 ± 0.176

0.715 ± 0.07

6.079 ± 0.121

7.844 ± 0.276

4.017 ± 0.109

6.598 ± 0.263

1.81 ± 0.099

6.456 ± 0.153

7.723 ± 0.208

7.751 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.098

5.45 ± 0.15

3.351 ± 0.14

3.84 ± 0.207

4.357 ± 0.13

6.042 ± 0.186

6.478 ± 0.239

6.833 ± 0.164

1.024 ± 0.067

4.228 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski