Ruminococcaceae bacterium D16

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4XGT0|F4XGT0_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium D16 OX=552398 GN=HMPREF0866_02831 PE=4 SV=1
MM1 pKa = 7.21FTPKK5 pKa = 10.31NIQGALEE12 pKa = 4.07EE13 pKa = 5.47LYY15 pKa = 10.79DD16 pKa = 4.61LCDD19 pKa = 3.77PDD21 pKa = 5.15YY22 pKa = 10.8MVDD25 pKa = 3.24MLVNYY30 pKa = 10.23SEE32 pKa = 4.6EE33 pKa = 4.12FDD35 pKa = 5.3DD36 pKa = 5.77ISPALLAKK44 pKa = 10.25SFQKK48 pKa = 9.71NAEE51 pKa = 4.23MISEE55 pKa = 4.04YY56 pKa = 10.38RR57 pKa = 11.84VLSSAGEE64 pKa = 4.39GIDD67 pKa = 3.7YY68 pKa = 10.26QGKK71 pKa = 9.06VLLNSRR77 pKa = 11.84AVRR80 pKa = 11.84LLSYY84 pKa = 10.81VEE86 pKa = 5.31DD87 pKa = 3.63MSDD90 pKa = 3.49DD91 pKa = 3.72EE92 pKa = 4.67KK93 pKa = 11.61VRR95 pKa = 11.84TIQSKK100 pKa = 7.86EE101 pKa = 3.44LWLAEE106 pKa = 4.02DD107 pKa = 3.22MTFYY111 pKa = 11.0VVSCMSTITMDD122 pKa = 3.5KK123 pKa = 11.1EE124 pKa = 3.65EE125 pKa = 4.92AICLNEE131 pKa = 3.85HH132 pKa = 6.46RR133 pKa = 11.84SVVTTVEE140 pKa = 4.18CEE142 pKa = 3.67DD143 pKa = 5.83DD144 pKa = 3.43IFFDD148 pKa = 3.85MGSLICEE155 pKa = 4.64LDD157 pKa = 4.38DD158 pKa = 3.63ICLFEE163 pKa = 5.52LLADD167 pKa = 4.03VDD169 pKa = 3.72ATIYY173 pKa = 10.61EE174 pKa = 4.33LL175 pKa = 4.55

Molecular weight: 19.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4XCC5|F4XCC5_9FIRM HTH tetR-type domain-containing protein OS=Ruminococcaceae bacterium D16 OX=552398 GN=HMPREF0866_01197 PE=4 SV=2
MM1 pKa = 7.89AGRR4 pKa = 11.84ASDD7 pKa = 3.62STRR10 pKa = 11.84RR11 pKa = 11.84TALGGVLAAGSLAVMWLACFFPTGKK36 pKa = 10.56LGLTAAAGLFPIGAVIAAGRR56 pKa = 11.84PAGLLCWAAASVLGLLLLPDD76 pKa = 3.76KK77 pKa = 10.94GVALMYY83 pKa = 10.72LCFLGLYY90 pKa = 6.42PTVKK94 pKa = 10.38SRR96 pKa = 11.84MEE98 pKa = 3.97NVKK101 pKa = 10.38SRR103 pKa = 11.84VVEE106 pKa = 4.06WVGKK110 pKa = 9.45FAFLNLALSICWFVFRR126 pKa = 11.84SLFLPALPQWIGEE139 pKa = 4.43KK140 pKa = 8.87TWLVYY145 pKa = 10.61LVGNVVFLIYY155 pKa = 10.5DD156 pKa = 3.61IGLSRR161 pKa = 11.84LIGAVMVRR169 pKa = 11.84LRR171 pKa = 11.84PGRR174 pKa = 11.84GRR176 pKa = 3.42

Molecular weight: 18.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2987

0

2987

924795

30

2776

309.6

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.075 ± 0.053

1.582 ± 0.022

5.559 ± 0.038

6.807 ± 0.05

3.775 ± 0.032

7.721 ± 0.047

1.815 ± 0.021

5.644 ± 0.036

4.999 ± 0.047

10.196 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.023

3.386 ± 0.035

4.159 ± 0.033

4.099 ± 0.03

5.338 ± 0.046

5.601 ± 0.034

5.583 ± 0.034

7.203 ± 0.042

1.13 ± 0.017

3.501 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski