Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps; Claviceps purpurea

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8814 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1WD09|M1WD09_CLAP2 Serine/threonine-protein phosphatase OS=Claviceps purpurea (strain 20.1) OX=1111077 GN=CPUR_08432 PE=3 SV=1
DDD2 pKa = 4.8EEE4 pKa = 4.36DD5 pKa = 4.28EEE7 pKa = 5.25DD8 pKa = 4.49EEE10 pKa = 5.42DD11 pKa = 4.53EEE13 pKa = 5.42DD14 pKa = 4.53EEE16 pKa = 5.42DD17 pKa = 4.53EEE19 pKa = 5.42DD20 pKa = 4.53EEE22 pKa = 5.42DD23 pKa = 4.53EEE25 pKa = 5.42DD26 pKa = 4.53EEE28 pKa = 5.42DD29 pKa = 4.53EEE31 pKa = 5.42DD32 pKa = 4.53EEE34 pKa = 5.42DD35 pKa = 4.53EEE37 pKa = 5.33DD38 pKa = 4.17EEE40 pKa = 5.8DD41 pKa = 3.63LVPTLLTLLWFLLKKK56 pKa = 10.05HH57 pKa = 5.88YYY59 pKa = 7.59TEEE62 pKa = 3.86LDDD65 pKa = 4.27FAKKK69 pKa = 9.84GCSTALTTSTLPADDD84 pKa = 3.95AAYYY88 pKa = 10.12SSLGLTVNRR97 pKa = 11.84YYY99 pKa = 8.26LCVTEEE105 pKa = 4.13EE106 pKa = 4.28FMGLAPLLAKKK117 pKa = 10.45EE118 pKa = 3.91DDD120 pKa = 4.08KK121 pKa = 10.78VVIHHH126 pKa = 6.6CDDD129 pKa = 3.0PYYY132 pKa = 10.5IRR134 pKa = 11.84RR135 pKa = 11.84TQEEE139 pKa = 3.32SHHH142 pKa = 5.28YY143 pKa = 9.85LVGEEE148 pKa = 4.62YYY150 pKa = 10.2HHH152 pKa = 6.04VMNGEEE158 pKa = 4.06TEEE161 pKa = 4.04GPEEE165 pKa = 3.84HHH167 pKa = 7.32DDD169 pKa = 3.27ILV

Molecular weight: 18.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1WET8|M1WET8_CLAP2 Uncharacterized protein OS=Claviceps purpurea (strain 20.1) OX=1111077 GN=CPUR_06956 PE=4 SV=1
MM1 pKa = 7.74LCTTCRR7 pKa = 11.84AARR10 pKa = 11.84QRR12 pKa = 11.84ASASASASASLLHH25 pKa = 6.68LPRR28 pKa = 11.84HH29 pKa = 5.07ITPVLRR35 pKa = 11.84RR36 pKa = 11.84PTTTTTQYY44 pKa = 11.17TSFTRR49 pKa = 11.84PTTPRR54 pKa = 11.84FFYY57 pKa = 9.34HH58 pKa = 5.2TTPPQRR64 pKa = 11.84QPQQASPEE72 pKa = 4.12QPSMSPAEE80 pKa = 3.83EE81 pKa = 4.59SIAEE85 pKa = 4.19RR86 pKa = 11.84LRR88 pKa = 11.84KK89 pKa = 9.07EE90 pKa = 4.06LQPTEE95 pKa = 4.07LLVRR99 pKa = 11.84DD100 pKa = 3.84VSGGCGSMYY109 pKa = 10.56AIDD112 pKa = 3.6IASPAFKK119 pKa = 10.2GLSMLKK125 pKa = 7.78QQRR128 pKa = 11.84MVNAALGEE136 pKa = 4.19LMKK139 pKa = 10.48GWHH142 pKa = 5.65GVQLNTRR149 pKa = 11.84VSKK152 pKa = 11.06

Molecular weight: 16.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8814

0

8814

4252061

10

8077

482.4

53.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.899 ± 0.024

1.277 ± 0.01

6.032 ± 0.02

6.198 ± 0.028

3.528 ± 0.014

6.78 ± 0.024

2.582 ± 0.012

4.568 ± 0.017

4.854 ± 0.024

8.796 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.009

3.48 ± 0.014

5.938 ± 0.026

4.205 ± 0.023

6.441 ± 0.022

8.341 ± 0.031

5.805 ± 0.018

6.023 ± 0.019

1.399 ± 0.008

2.564 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski