Lachnoclostridium sp. An298

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4B3Z5|A0A1Y4B3Z5_9FIRM Flavin reductase OS=Lachnoclostridium sp. An298 OX=1965627 GN=B5F86_10060 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84KK3 pKa = 9.52SKK5 pKa = 10.74LLICALFMSLILTALPGMEE24 pKa = 4.55SYY26 pKa = 11.11AAGEE30 pKa = 4.37TGEE33 pKa = 4.76SAAQTDD39 pKa = 4.13LQDD42 pKa = 3.65EE43 pKa = 4.32NGQRR47 pKa = 11.84LEE49 pKa = 4.34TVTAMDD55 pKa = 3.89EE56 pKa = 3.98NGNIYY61 pKa = 10.36EE62 pKa = 4.49VGDD65 pKa = 3.43SDD67 pKa = 4.04GTVGEE72 pKa = 4.62SAAEE76 pKa = 4.37DD77 pKa = 3.97GSEE80 pKa = 4.02EE81 pKa = 4.21GTDD84 pKa = 3.5AEE86 pKa = 4.61SGISLFSARR95 pKa = 11.84SAAVSMKK102 pKa = 10.08VVNFNTKK109 pKa = 10.12SNATTDD115 pKa = 3.86FTYY118 pKa = 10.71SGGSGYY124 pKa = 9.47TNGDD128 pKa = 3.35YY129 pKa = 11.2GADD132 pKa = 3.39AAYY135 pKa = 10.6LGTDD139 pKa = 3.39GSGDD143 pKa = 3.38IRR145 pKa = 11.84FMLAGVTGTVKK156 pKa = 10.35KK157 pKa = 10.93SEE159 pKa = 4.24VEE161 pKa = 4.15VVDD164 pKa = 3.97YY165 pKa = 11.45SSVADD170 pKa = 3.41NVSYY174 pKa = 11.44YY175 pKa = 10.19EE176 pKa = 3.96VSGGQLIHH184 pKa = 6.54YY185 pKa = 9.89ISYY188 pKa = 10.55NLNEE192 pKa = 4.88GPSSSINNGPAPSYY206 pKa = 10.31LSEE209 pKa = 3.88GVKK212 pKa = 10.03YY213 pKa = 10.51FSYY216 pKa = 10.45DD217 pKa = 2.58GHH219 pKa = 7.06YY220 pKa = 10.29FYY222 pKa = 11.28EE223 pKa = 4.91DD224 pKa = 3.89YY225 pKa = 10.04KK226 pKa = 11.7TMLNDD231 pKa = 3.51YY232 pKa = 10.74QSGSNGASAVNCGNAFYY249 pKa = 10.93NYY251 pKa = 10.01FQFLDD256 pKa = 3.89MNSSTNYY263 pKa = 10.63SGDD266 pKa = 3.6EE267 pKa = 3.96LDD269 pKa = 5.37SILSSKK275 pKa = 10.27ISSSSSKK282 pKa = 10.92LLGTGTLFVKK292 pKa = 9.97YY293 pKa = 8.71QNKK296 pKa = 9.83YY297 pKa = 9.8SVNALLSLGIAVNEE311 pKa = 4.51SAWGTSSICMDD322 pKa = 3.57KK323 pKa = 11.51NNLFGLNAVDD333 pKa = 3.94TSPGQSANYY342 pKa = 7.86FASVEE347 pKa = 4.04DD348 pKa = 4.6CIRR351 pKa = 11.84EE352 pKa = 4.03FMSEE356 pKa = 3.74WMADD360 pKa = 3.67GYY362 pKa = 11.15LSSSDD367 pKa = 2.97WRR369 pKa = 11.84NHH371 pKa = 6.07GEE373 pKa = 3.81LLGNKK378 pKa = 9.9GEE380 pKa = 4.83GINVSYY386 pKa = 11.09ASDD389 pKa = 4.16PYY391 pKa = 10.24WGEE394 pKa = 3.58KK395 pKa = 9.27AAAIAWEE402 pKa = 4.15LDD404 pKa = 3.46AAGGSHH410 pKa = 7.91DD411 pKa = 4.16YY412 pKa = 11.27LGISSGSGNEE422 pKa = 3.96SSEE425 pKa = 4.78DD426 pKa = 3.54NTDD429 pKa = 3.51TPSEE433 pKa = 4.3DD434 pKa = 3.43NMPPSDD440 pKa = 5.13AGGTDD445 pKa = 3.59DD446 pKa = 6.21ANDD449 pKa = 3.34SSAADD454 pKa = 3.81NDD456 pKa = 4.03SDD458 pKa = 3.85TDD460 pKa = 3.88DD461 pKa = 4.55PSEE464 pKa = 4.14EE465 pKa = 4.64DD466 pKa = 3.52SDD468 pKa = 4.79SEE470 pKa = 4.34ADD472 pKa = 3.64SSVDD476 pKa = 3.14TGNGSEE482 pKa = 4.55TEE484 pKa = 4.23NPTDD488 pKa = 3.69ANEE491 pKa = 4.35PSDD494 pKa = 4.0TNDD497 pKa = 3.15PTDD500 pKa = 3.68ANEE503 pKa = 4.47PSDD506 pKa = 4.08TNDD509 pKa = 3.2PTDD512 pKa = 3.48TNEE515 pKa = 4.24PSDD518 pKa = 3.35TGTPSEE524 pKa = 4.67EE525 pKa = 4.34EE526 pKa = 4.0NDD528 pKa = 3.8SEE530 pKa = 4.45TDD532 pKa = 3.32GGVDD536 pKa = 3.48EE537 pKa = 5.25EE538 pKa = 4.69NGSEE542 pKa = 5.09AEE544 pKa = 4.76DD545 pKa = 3.42PTDD548 pKa = 3.88ANEE551 pKa = 4.4PSDD554 pKa = 4.72ANDD557 pKa = 3.69PADD560 pKa = 3.74TNEE563 pKa = 5.1PIDD566 pKa = 3.69TNKK569 pKa = 10.28PVDD572 pKa = 3.58TDD574 pKa = 3.87TPSDD578 pKa = 3.56EE579 pKa = 4.59SSNSGADD586 pKa = 3.58KK587 pKa = 10.96KK588 pKa = 10.81PADD591 pKa = 4.04TPADD595 pKa = 3.99RR596 pKa = 11.84QLADD600 pKa = 3.28IDD602 pKa = 4.23HH603 pKa = 6.39GVSVSGRR610 pKa = 11.84FSEE613 pKa = 4.95DD614 pKa = 2.71AALNVQAVEE623 pKa = 4.32PNTDD627 pKa = 2.76QYY629 pKa = 10.79ATYY632 pKa = 9.26VTADD636 pKa = 3.57PVKK639 pKa = 9.42GKK641 pKa = 10.47VVLGVYY647 pKa = 10.31DD648 pKa = 3.44ITLTGTLEE656 pKa = 4.41GEE658 pKa = 4.09AQLTFRR664 pKa = 11.84VGTEE668 pKa = 3.68YY669 pKa = 10.89NGRR672 pKa = 11.84NVIILHH678 pKa = 5.59YY679 pKa = 10.25ADD681 pKa = 4.84DD682 pKa = 4.62GSYY685 pKa = 8.47EE686 pKa = 4.18TYY688 pKa = 10.35KK689 pKa = 11.01AAVEE693 pKa = 4.04NGQVTISVKK702 pKa = 10.36GFSPYY707 pKa = 10.53VIALDD712 pKa = 4.32DD713 pKa = 4.86ANSSSSIEE721 pKa = 3.94NKK723 pKa = 10.26APQTGDD729 pKa = 3.03TAEE732 pKa = 4.22PGIWIALMGMAIVLGAAAIFKK753 pKa = 10.16KK754 pKa = 10.39RR755 pKa = 11.84AVRR758 pKa = 3.75

Molecular weight: 80.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4AQ20|A0A1Y4AQ20_9FIRM Uncharacterized protein OS=Lachnoclostridium sp. An298 OX=1965627 GN=B5F86_15180 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2948

0

2948

916666

36

2524

310.9

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.644 ± 0.045

1.52 ± 0.02

5.928 ± 0.04

7.892 ± 0.049

3.941 ± 0.035

7.294 ± 0.039

1.749 ± 0.02

7.278 ± 0.043

6.282 ± 0.043

8.781 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.128 ± 0.023

3.954 ± 0.026

3.411 ± 0.026

3.143 ± 0.025

5.035 ± 0.035

5.698 ± 0.036

5.355 ± 0.029

6.871 ± 0.038

0.922 ± 0.017

4.172 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski