Klebsiella phage KMI6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NBB6|A0A5B9NBB6_9CAUD Uncharacterized protein OS=Klebsiella phage KMI6 OX=2601617 GN=KMI6_26 PE=4 SV=1
MM1 pKa = 7.26VNVFNIIVTSAMLVLGNDD19 pKa = 3.45VNNPVPYY26 pKa = 9.36CTVQLQQPAEE36 pKa = 4.13QEE38 pKa = 3.99PQPRR42 pKa = 11.84PEE44 pKa = 3.92YY45 pKa = 10.91DD46 pKa = 3.21LFEE49 pKa = 6.24DD50 pKa = 4.38PEE52 pKa = 4.87GGCKK56 pKa = 9.76EE57 pKa = 3.82LGARR61 pKa = 11.84ILAAVQEE68 pKa = 4.55QYY70 pKa = 11.4PNAAVTLTVDD80 pKa = 3.9GKK82 pKa = 11.4SNNDD86 pKa = 2.72II87 pKa = 4.2

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NF64|A0A5B9NF64_9CAUD Uncharacterized protein OS=Klebsiella phage KMI6 OX=2601617 GN=KMI6_22 PE=4 SV=1
MM1 pKa = 8.03KK2 pKa = 10.36YY3 pKa = 9.7RR4 pKa = 11.84EE5 pKa = 4.05KK6 pKa = 10.7LAQQFAGLEE15 pKa = 4.1KK16 pKa = 10.83LSGSEE21 pKa = 3.56LRR23 pKa = 11.84KK24 pKa = 10.13RR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 3.28ALNRR31 pKa = 11.84AGYY34 pKa = 8.53MRR36 pKa = 11.84TKK38 pKa = 10.51QSATFSTNVRR48 pKa = 11.84GKK50 pKa = 8.48TKK52 pKa = 10.34TKK54 pKa = 10.18GSSKK58 pKa = 10.59APQGWYY64 pKa = 8.26TAGQFGRR71 pKa = 4.16

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

14097

60

1232

247.3

27.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.258 ± 0.508

1.447 ± 0.229

6.023 ± 0.215

5.71 ± 0.3

3.05 ± 0.163

7.604 ± 0.247

1.929 ± 0.185

4.526 ± 0.174

4.675 ± 0.288

8.64 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.214

4.157 ± 0.219

3.838 ± 0.233

4.81 ± 0.371

6.072 ± 0.264

6.406 ± 0.223

5.753 ± 0.239

6.98 ± 0.285

1.397 ± 0.104

3.802 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski