Siphonobacter sp. BAB-5385

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Siphonobacter; unclassified Siphonobacter

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5095 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261Q2Q8|A0A261Q2Q8_9BACT Ribosomal protein L11 methyltransferase OS=Siphonobacter sp. BAB-5385 OX=1864822 GN=prmA PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.31LFRR5 pKa = 11.84YY6 pKa = 8.29ITDD9 pKa = 3.35EE10 pKa = 4.09DD11 pKa = 4.53VVPGILLDD19 pKa = 3.95GKK21 pKa = 10.57AYY23 pKa = 10.38SLEE26 pKa = 4.09AFFEE30 pKa = 4.33ALGEE34 pKa = 4.1EE35 pKa = 4.27TGAYY39 pKa = 10.17DD40 pKa = 3.79LDD42 pKa = 4.18EE43 pKa = 4.69IFFEE47 pKa = 4.46EE48 pKa = 4.31EE49 pKa = 3.95GLSVLEE55 pKa = 5.75DD56 pKa = 3.5YY57 pKa = 11.24LEE59 pKa = 4.19QYY61 pKa = 7.53GTSEE65 pKa = 4.18LQALEE70 pKa = 4.25GPIEE74 pKa = 4.14YY75 pKa = 9.64ASCVARR81 pKa = 11.84PSKK84 pKa = 10.33IICIGLNYY92 pKa = 9.74ADD94 pKa = 4.42HH95 pKa = 7.05AKK97 pKa = 8.13EE98 pKa = 4.07TNAPIPQEE106 pKa = 3.96PIVFFKK112 pKa = 10.61ATTALCGPNDD122 pKa = 3.9DD123 pKa = 5.17VIIPRR128 pKa = 11.84NSVKK132 pKa = 10.09TDD134 pKa = 3.12WEE136 pKa = 4.2VEE138 pKa = 4.01LAVVIGAEE146 pKa = 3.99CSYY149 pKa = 9.68VTEE152 pKa = 4.83EE153 pKa = 3.97EE154 pKa = 4.3AEE156 pKa = 4.26DD157 pKa = 4.29YY158 pKa = 10.63IAGYY162 pKa = 11.03AVMNDD167 pKa = 3.12YY168 pKa = 11.04SEE170 pKa = 6.23RR171 pKa = 11.84EE172 pKa = 3.94FQLEE176 pKa = 5.49RR177 pKa = 11.84GGQWDD182 pKa = 3.74KK183 pKa = 11.9GKK185 pKa = 11.04GCDD188 pKa = 3.33TFGPMGPYY196 pKa = 10.34LVTPDD201 pKa = 4.2EE202 pKa = 4.74LDD204 pKa = 4.38DD205 pKa = 4.38VDD207 pKa = 5.94SLPMWLKK214 pKa = 11.22VNGQTYY220 pKa = 10.36QNSTTAQMIFKK231 pKa = 9.55VPQIISYY238 pKa = 10.18LSQFMTLLPGDD249 pKa = 4.39VISTGTPPGVGLGFNPPVYY268 pKa = 10.13LKK270 pKa = 10.74PGDD273 pKa = 3.67IVEE276 pKa = 4.63LGIEE280 pKa = 4.2GLGEE284 pKa = 3.81QRR286 pKa = 11.84QVAVSWEE293 pKa = 3.79DD294 pKa = 3.31AYY296 pKa = 11.08

Molecular weight: 32.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261Q4K3|A0A261Q4K3_9BACT Uncharacterized protein (Fragment) OS=Siphonobacter sp. BAB-5385 OX=1864822 GN=BWI93_13420 PE=4 SV=1
MM1 pKa = 7.33QYY3 pKa = 9.52VAYY6 pKa = 10.48YY7 pKa = 9.91RR8 pKa = 11.84VSTLAQGKK16 pKa = 9.79SGLGLAAQQEE26 pKa = 4.41IVRR29 pKa = 11.84RR30 pKa = 11.84FVRR33 pKa = 11.84EE34 pKa = 4.09GEE36 pKa = 4.18VLLQEE41 pKa = 4.3FVEE44 pKa = 4.47VEE46 pKa = 3.99SGKK49 pKa = 10.56KK50 pKa = 8.88SEE52 pKa = 4.14RR53 pKa = 11.84PQLKK57 pKa = 10.03AAIAFAKK64 pKa = 7.72QHH66 pKa = 6.14RR67 pKa = 11.84ARR69 pKa = 11.84LLIAKK74 pKa = 9.81LDD76 pKa = 3.83RR77 pKa = 11.84LSRR80 pKa = 11.84NAEE83 pKa = 4.17FIFTLRR89 pKa = 11.84NAEE92 pKa = 3.99VDD94 pKa = 5.12FVACDD99 pKa = 3.27IPDD102 pKa = 4.11ANTLTIGIMAVLAQHH117 pKa = 5.49EE118 pKa = 4.59RR119 pKa = 11.84EE120 pKa = 4.59LISEE124 pKa = 4.58RR125 pKa = 11.84TRR127 pKa = 11.84NALQQKK133 pKa = 9.02KK134 pKa = 9.1RR135 pKa = 11.84QGYY138 pKa = 8.92SLGSPEE144 pKa = 4.85NLTSQAIDD152 pKa = 3.04KK153 pKa = 10.51GRR155 pKa = 11.84QQRR158 pKa = 11.84IANARR163 pKa = 11.84THH165 pKa = 5.02QANRR169 pKa = 11.84QAAEE173 pKa = 3.8LARR176 pKa = 11.84LYY178 pKa = 10.68RR179 pKa = 11.84NQGLTYY185 pKa = 11.09ALIAEE190 pKa = 4.53KK191 pKa = 10.93LNANGFRR198 pKa = 11.84TRR200 pKa = 11.84RR201 pKa = 11.84GQIFHH206 pKa = 6.9PMSVRR211 pKa = 11.84RR212 pKa = 11.84LILRR216 pKa = 11.84PEE218 pKa = 3.74AA219 pKa = 4.29

Molecular weight: 24.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5095

0

5095

1708927

26

2130

335.4

37.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.744 ± 0.037

0.707 ± 0.01

4.931 ± 0.027

5.957 ± 0.039

4.642 ± 0.025

6.921 ± 0.034

1.929 ± 0.017

5.787 ± 0.029

5.502 ± 0.034

10.26 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.014

4.54 ± 0.032

4.417 ± 0.022

4.743 ± 0.027

5.201 ± 0.025

6.294 ± 0.026

6.211 ± 0.032

6.686 ± 0.023

1.427 ± 0.015

4.013 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski