Gemmata obscuriglobus

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Gemmatales; Gemmataceae; Gemmata

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3GZA3|A0A2Z3GZA3_9BACT FliMN_C domain-containing protein OS=Gemmata obscuriglobus OX=114 GN=C1280_10415 PE=3 SV=1
MM1 pKa = 7.38SSARR5 pKa = 11.84SFSHH9 pKa = 7.21RR10 pKa = 11.84APQFVPAVEE19 pKa = 4.09GLEE22 pKa = 5.01DD23 pKa = 3.51RR24 pKa = 11.84TTPAGNVTAYY34 pKa = 10.81VFDD37 pKa = 3.64KK38 pKa = 10.88VLYY41 pKa = 8.9VTGTNDD47 pKa = 3.71TDD49 pKa = 4.01QIWIAGAGEE58 pKa = 3.94NAVVIRR64 pKa = 11.84SLDD67 pKa = 3.31GATTINGQSSVYY79 pKa = 10.05IGGVKK84 pKa = 9.6QGYY87 pKa = 8.86HH88 pKa = 6.12INMGGGDD95 pKa = 4.15DD96 pKa = 3.88TLLVTGTRR104 pKa = 11.84SGGGLNVDD112 pKa = 3.51MGVGNDD118 pKa = 3.35VLGISDD124 pKa = 4.46AGHH127 pKa = 6.72KK128 pKa = 10.2GGSVLTGGAGNDD140 pKa = 3.39VFVLHH145 pKa = 7.23ASGFRR150 pKa = 11.84NGVSLNTGDD159 pKa = 5.34GDD161 pKa = 4.15DD162 pKa = 3.64QVLVSGVSAPAFALVNPSGTDD183 pKa = 3.32YY184 pKa = 11.33FDD186 pKa = 4.66APGSALGRR194 pKa = 11.84PSVVGGFVPGSPTATVPPTAAAADD218 pKa = 4.26TVAPTATVSTFSASVTSTAAVPFTVTFDD246 pKa = 3.53EE247 pKa = 4.87DD248 pKa = 3.98VTGFTAAGLTVSNGTVTAFGAVNARR273 pKa = 11.84TYY275 pKa = 9.24TFSVVPSGQGAVSVSVAAGAAQDD298 pKa = 3.61AAGNANTASNTVAVTFDD315 pKa = 3.43SVAPAVTVNSLTSSSATPTLTGTVDD340 pKa = 3.61DD341 pKa = 5.01PNATVSVTVNGQTYY355 pKa = 9.41KK356 pKa = 10.33ATVSSTTWSVTLTNPLTDD374 pKa = 3.37GTFTISVSATDD385 pKa = 3.17AAGNVGGTSLTDD397 pKa = 3.45GLVVSLAGLTVTVDD411 pKa = 3.47TLATNSTTPTITGTVSDD428 pKa = 3.96ATATVQVTVDD438 pKa = 3.39GQTYY442 pKa = 7.14TATVSGTTWSVAVTTALVEE461 pKa = 4.06GTYY464 pKa = 9.51TVSATATDD472 pKa = 3.76TNGNVATASPADD484 pKa = 3.73GLVIDD489 pKa = 4.96LTTPTVTANALTTNSATPTLTGTVDD514 pKa = 3.57DD515 pKa = 4.26STAAVQVTVDD525 pKa = 3.67GQAYY529 pKa = 6.05TATVSGTTWSVSVPTALIEE548 pKa = 4.03GTYY551 pKa = 8.93TISVTAMDD559 pKa = 3.75AAGNVGAASLTGGLVVDD576 pKa = 4.28LTVPTVTINALITNSTTPTLTGTVSDD602 pKa = 3.78STATVQVTVDD612 pKa = 3.38GQTYY616 pKa = 8.07PATVSGNTWTVAITTALVEE635 pKa = 4.34GAYY638 pKa = 8.15TASVTATDD646 pKa = 3.39VAGNVGTASLTDD658 pKa = 3.7GVVVDD663 pKa = 4.14LTVPTVAVDD672 pKa = 3.79TVPTVAGAVTGTAGDD687 pKa = 3.79TLAGVTGVAVTIYY700 pKa = 10.93DD701 pKa = 3.92SVAQAYY707 pKa = 10.3LNASRR712 pKa = 11.84TAFDD716 pKa = 3.33SATEE720 pKa = 3.9VWITAEE726 pKa = 3.99TADD729 pKa = 5.58AFATWSVTVPVAGSYY744 pKa = 9.4VVVAEE749 pKa = 4.31ATDD752 pKa = 4.06GAGNTATGNTTAVTSS767 pKa = 3.87

Molecular weight: 75.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3GUD3|A0A2Z3GUD3_9BACT SBP_bac_5 domain-containing protein OS=Gemmata obscuriglobus OX=114 GN=C1280_14195 PE=4 SV=1
MM1 pKa = 7.23VGRR4 pKa = 11.84GRR6 pKa = 11.84ARR8 pKa = 11.84VGTRR12 pKa = 11.84RR13 pKa = 11.84FVSVVLRR20 pKa = 11.84VAPVGGGRR28 pKa = 11.84GSSPRR33 pKa = 11.84VAVVGGLRR41 pKa = 11.84CVATRR46 pKa = 11.84RR47 pKa = 11.84IGLGVLRR54 pKa = 11.84PAAPVARR61 pKa = 11.84APRR64 pKa = 11.84SRR66 pKa = 11.84GAARR70 pKa = 11.84VARR73 pKa = 11.84NRR75 pKa = 11.84GGQQ78 pKa = 3.06

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6809

0

6809

2352850

26

6552

345.6

37.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.595 ± 0.048

1.06 ± 0.012

5.651 ± 0.019

5.582 ± 0.027

3.541 ± 0.014

8.759 ± 0.032

2.05 ± 0.013

3.255 ± 0.021

3.989 ± 0.031

9.93 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.655 ± 0.01

2.556 ± 0.021

6.408 ± 0.028

2.906 ± 0.018

7.501 ± 0.033

4.707 ± 0.024

5.81 ± 0.036

8.298 ± 0.024

1.52 ± 0.012

2.227 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski