Macrococcus lamae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Macrococcus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2076 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6BXH3|A0A4R6BXH3_9STAP Tetratricopeptide repeat protein OS=Macrococcus lamae OX=198484 GN=ERX29_00230 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 5.25IYY4 pKa = 10.98HH5 pKa = 7.24IIDD8 pKa = 3.62KK9 pKa = 10.76VHH11 pKa = 5.88LQKK14 pKa = 10.23IEE16 pKa = 4.38QIDD19 pKa = 3.74NDD21 pKa = 3.81INDD24 pKa = 4.09ALMSEE29 pKa = 4.2DD30 pKa = 3.85HH31 pKa = 6.86EE32 pKa = 4.84GLFVLGEE39 pKa = 3.92TLYY42 pKa = 10.88QYY44 pKa = 11.8GLVPQGMKK52 pKa = 10.33VFEE55 pKa = 4.54ALYY58 pKa = 9.23HH59 pKa = 5.35QYY61 pKa = 10.58PEE63 pKa = 4.41EE64 pKa = 5.21IEE66 pKa = 4.12LLVYY70 pKa = 10.48YY71 pKa = 10.11IEE73 pKa = 4.6GLIDD77 pKa = 3.34EE78 pKa = 4.5NQIDD82 pKa = 3.81KK83 pKa = 11.05ALEE86 pKa = 4.17ILHH89 pKa = 6.5HH90 pKa = 6.32APKK93 pKa = 8.32TTEE96 pKa = 3.92RR97 pKa = 11.84LLLEE101 pKa = 4.45ADD103 pKa = 4.33VYY105 pKa = 10.15QQQDD109 pKa = 4.0MIEE112 pKa = 4.12VALDD116 pKa = 3.43KK117 pKa = 11.14LKK119 pKa = 10.92SAEE122 pKa = 4.09EE123 pKa = 4.03LAPDD127 pKa = 4.11DD128 pKa = 5.59LIITFALAEE137 pKa = 4.35LFYY140 pKa = 11.06FDD142 pKa = 5.07AQYY145 pKa = 11.06LQAARR150 pKa = 11.84RR151 pKa = 11.84YY152 pKa = 7.21EE153 pKa = 3.9TLLNSGEE160 pKa = 4.02QVINHH165 pKa = 5.4VNVNSRR171 pKa = 11.84LADD174 pKa = 3.43AMLQSGDD181 pKa = 3.67YY182 pKa = 8.96EE183 pKa = 3.91QAEE186 pKa = 4.58IYY188 pKa = 10.71FNTLEE193 pKa = 4.15EE194 pKa = 4.25QEE196 pKa = 4.25MTSDD200 pKa = 3.49DD201 pKa = 4.28FFRR204 pKa = 11.84KK205 pKa = 10.26AIALQKK211 pKa = 10.28NDD213 pKa = 4.33HH214 pKa = 6.43VPDD217 pKa = 5.7AIQVLNTLLEE227 pKa = 4.2KK228 pKa = 11.06DD229 pKa = 3.91PDD231 pKa = 3.84YY232 pKa = 11.06MHH234 pKa = 7.79AYY236 pKa = 9.82LLLVNLYY243 pKa = 9.16EE244 pKa = 4.62AEE246 pKa = 4.07RR247 pKa = 11.84LYY249 pKa = 11.32DD250 pKa = 3.65EE251 pKa = 6.14AILIGKK257 pKa = 8.97QGLALNEE264 pKa = 4.07FFKK267 pKa = 11.14EE268 pKa = 3.98LLVDD272 pKa = 3.82TARR275 pKa = 11.84VMLHH279 pKa = 6.07TKK281 pKa = 9.05NQEE284 pKa = 3.6AEE286 pKa = 4.01QYY288 pKa = 10.37LIQALTIDD296 pKa = 3.85PSYY299 pKa = 11.35TEE301 pKa = 4.1AALLLSDD308 pKa = 4.22YY309 pKa = 11.07YY310 pKa = 11.22KK311 pKa = 10.91EE312 pKa = 3.9NDD314 pKa = 3.33MFEE317 pKa = 4.01EE318 pKa = 4.42SVRR321 pKa = 11.84LFSLIDD327 pKa = 3.7EE328 pKa = 4.62EE329 pKa = 6.78DD330 pKa = 3.12IDD332 pKa = 6.51PIVKK336 pKa = 9.53WNLGYY341 pKa = 10.44SYY343 pKa = 10.89QQLEE347 pKa = 4.08RR348 pKa = 11.84DD349 pKa = 3.49NEE351 pKa = 4.06AKK353 pKa = 10.41HH354 pKa = 6.42FYY356 pKa = 8.7EE357 pKa = 4.4QAYY360 pKa = 10.68ADD362 pKa = 4.42LSDD365 pKa = 4.77NLDD368 pKa = 3.45FLYY371 pKa = 10.44DD372 pKa = 3.49YY373 pKa = 11.02HH374 pKa = 8.32DD375 pKa = 4.11YY376 pKa = 11.12LLEE379 pKa = 5.96IGEE382 pKa = 4.61DD383 pKa = 3.54TTKK386 pKa = 10.3VRR388 pKa = 11.84SQLTALDD395 pKa = 4.35PGFDD399 pKa = 3.42PYY401 pKa = 11.38EE402 pKa = 3.92RR403 pKa = 5.25

Molecular weight: 47.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6BTK1|A0A4R6BTK1_9STAP MFS transporter OS=Macrococcus lamae OX=198484 GN=ERX29_07815 PE=4 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.91VHH17 pKa = 5.68GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 9.66NGRR29 pKa = 11.84RR30 pKa = 11.84VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2076

0

2076

610545

21

1418

294.1

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.874 ± 0.054

0.644 ± 0.016

5.747 ± 0.045

7.027 ± 0.058

4.509 ± 0.049

6.378 ± 0.059

2.342 ± 0.03

8.137 ± 0.056

6.714 ± 0.053

9.573 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.037 ± 0.025

4.73 ± 0.036

3.368 ± 0.029

3.74 ± 0.032

4.055 ± 0.038

5.793 ± 0.038

5.723 ± 0.04

6.967 ± 0.043

0.781 ± 0.016

3.86 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski