Cucurbit yellow mosaic alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; unclassified Begomovirus-associated alphasatellites

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A145PS99|A0A145PS99_9VIRU Alpha-V2 OS=Cucurbit yellow mosaic alphasatellite OX=1819678 PE=4 SV=1
MM1 pKa = 6.91SHH3 pKa = 5.9SRR5 pKa = 11.84NWCFTIFNYY14 pKa = 9.97VLPLFTTLPEE24 pKa = 3.74WANYY28 pKa = 10.17LIYY31 pKa = 10.33QEE33 pKa = 4.96EE34 pKa = 4.52EE35 pKa = 4.21CPTTKK40 pKa = 9.85KK41 pKa = 10.48RR42 pKa = 11.84HH43 pKa = 4.56IQGYY47 pKa = 9.82VNLKK51 pKa = 9.19RR52 pKa = 11.84NQRR55 pKa = 11.84FAFLKK60 pKa = 10.51KK61 pKa = 9.93KK62 pKa = 10.72LPDD65 pKa = 3.6GTHH68 pKa = 6.52IEE70 pKa = 4.1ACKK73 pKa = 10.49GSSSSNRR80 pKa = 11.84DD81 pKa = 3.1YY82 pKa = 11.17CRR84 pKa = 11.84KK85 pKa = 10.24DD86 pKa = 3.2DD87 pKa = 3.89TRR89 pKa = 11.84TDD91 pKa = 3.98GPWEE95 pKa = 3.98FGVFAEE101 pKa = 4.51TGSNKK106 pKa = 9.69RR107 pKa = 11.84KK108 pKa = 7.94TMEE111 pKa = 4.3RR112 pKa = 11.84FQEE115 pKa = 4.28DD116 pKa = 4.08PEE118 pKa = 4.17EE119 pKa = 4.61LRR121 pKa = 11.84LADD124 pKa = 3.63PKK126 pKa = 10.93LYY128 pKa = 10.25RR129 pKa = 11.84RR130 pKa = 11.84CLATKK135 pKa = 10.38VNTEE139 pKa = 3.77FGGLVLPVPDD149 pKa = 5.19RR150 pKa = 11.84PWQLVAQKK158 pKa = 10.87VLDD161 pKa = 3.75QGPDD165 pKa = 3.04DD166 pKa = 4.0RR167 pKa = 11.84TIIWVYY173 pKa = 10.5GSEE176 pKa = 4.1GNEE179 pKa = 4.89GKK181 pKa = 7.14TTWAKK186 pKa = 10.08TKK188 pKa = 10.47IQDD191 pKa = 2.55GWFYY195 pKa = 11.41SRR197 pKa = 11.84GGKK200 pKa = 9.87GEE202 pKa = 4.13NIKK205 pKa = 10.35YY206 pKa = 10.12QYY208 pKa = 10.88AEE210 pKa = 4.27HH211 pKa = 6.93LGHH214 pKa = 7.3CIFDD218 pKa = 3.49IPRR221 pKa = 11.84QVEE224 pKa = 4.24DD225 pKa = 3.31NLQYY229 pKa = 10.79TVLEE233 pKa = 4.5EE234 pKa = 3.95IKK236 pKa = 10.78DD237 pKa = 3.69RR238 pKa = 11.84LIRR241 pKa = 11.84SSKK244 pKa = 10.56YY245 pKa = 10.02EE246 pKa = 4.51PIDD249 pKa = 3.78FNCSDD254 pKa = 3.55KK255 pKa = 11.12VHH257 pKa = 6.12VVVLSNFLPCLDD269 pKa = 3.67SEE271 pKa = 4.74YY272 pKa = 10.93NNRR275 pKa = 11.84GEE277 pKa = 4.38LVKK280 pKa = 10.8KK281 pKa = 10.14PLLSRR286 pKa = 11.84DD287 pKa = 3.15RR288 pKa = 11.84VFLINIDD295 pKa = 3.84EE296 pKa = 4.66SVCGHH301 pKa = 7.31PDD303 pKa = 3.47DD304 pKa = 4.95LTAFDD309 pKa = 5.59LYY311 pKa = 11.09LSPEE315 pKa = 3.86

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A145PS99|A0A145PS99_9VIRU Alpha-V2 OS=Cucurbit yellow mosaic alphasatellite OX=1819678 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 6.61PILKK6 pKa = 9.07PARR9 pKa = 11.84VHH11 pKa = 6.43HH12 pKa = 6.07PAIEE16 pKa = 4.62IIAEE20 pKa = 4.06RR21 pKa = 11.84MIQGLTVHH29 pKa = 6.78GSLEE33 pKa = 4.06FSRR36 pKa = 11.84KK37 pKa = 8.9QGATRR42 pKa = 11.84GRR44 pKa = 11.84RR45 pKa = 11.84WNVFRR50 pKa = 11.84KK51 pKa = 7.48TLKK54 pKa = 10.67NLDD57 pKa = 3.94WPTLNCIVAAWRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84LIRR74 pKa = 11.84SSVVWYY80 pKa = 10.5SLYY83 pKa = 10.2LTDD86 pKa = 6.14LGSSWLRR93 pKa = 11.84RR94 pKa = 11.84YY95 pKa = 6.34WTRR98 pKa = 11.84AQMIEE103 pKa = 4.04LSYY106 pKa = 11.76GCMALKK112 pKa = 10.53AMKK115 pKa = 10.05AKK117 pKa = 10.23PHH119 pKa = 5.53GLRR122 pKa = 11.84PRR124 pKa = 11.84FRR126 pKa = 11.84MGGSTQGEE134 pKa = 4.28EE135 pKa = 4.05RR136 pKa = 11.84EE137 pKa = 4.4KK138 pKa = 10.81II139 pKa = 3.64

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

454

139

315

227.0

26.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.405 ± 1.484

2.423 ± 0.523

5.507 ± 2.164

7.048 ± 0.688

3.965 ± 0.961

6.388 ± 0.429

2.423 ± 0.242

5.286 ± 0.632

6.828 ± 0.571

9.471 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 1.359

4.185 ± 1.078

5.066 ± 0.399

3.304 ± 0.227

8.59 ± 2.319

5.727 ± 0.398

5.507 ± 0.25

5.286 ± 0.516

2.863 ± 0.773

3.965 ± 0.578
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski