Mycobacterium phage Yuna

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TG24|A0A5J6TG24_9CAUD Uncharacterized protein OS=Mycobacterium phage Yuna OX=2599885 GN=16 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.35APSTIEE8 pKa = 4.15VPCPACGEE16 pKa = 4.48PIVLAIGFEE25 pKa = 4.41VEE27 pKa = 4.29MPEE30 pKa = 4.52PGADD34 pKa = 3.34SAPVRR39 pKa = 11.84ITTPDD44 pKa = 3.27LGDD47 pKa = 3.15QARR50 pKa = 11.84AHH52 pKa = 6.51GEE54 pKa = 4.09VCSVLTAGGAA64 pKa = 3.3

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TL66|A0A5J6TL66_9CAUD Uncharacterized protein OS=Mycobacterium phage Yuna OX=2599885 GN=90 PE=4 SV=1
MM1 pKa = 7.44SKK3 pKa = 10.51ARR5 pKa = 11.84NATGQMSLDD14 pKa = 3.73GLDD17 pKa = 3.22AGMMFVAEE25 pKa = 4.36PATIRR30 pKa = 11.84RR31 pKa = 11.84PRR33 pKa = 11.84PAVVAAPAKK42 pKa = 7.53PAHH45 pKa = 6.04MAMSEE50 pKa = 3.89ARR52 pKa = 11.84RR53 pKa = 11.84MTEE56 pKa = 3.43RR57 pKa = 11.84LLAEE61 pKa = 4.75HH62 pKa = 6.91GLIGWTVTFDD72 pKa = 3.33NARR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84AGVCRR82 pKa = 11.84YY83 pKa = 7.22TPKK86 pKa = 10.62QIGLSKK92 pKa = 10.53PLMAQRR98 pKa = 11.84SYY100 pKa = 11.83DD101 pKa = 3.63DD102 pKa = 3.51TVQTITHH109 pKa = 7.22EE110 pKa = 4.09IAHH113 pKa = 6.58ALVGHH118 pKa = 5.84KK119 pKa = 9.86HH120 pKa = 4.99GHH122 pKa = 5.78DD123 pKa = 3.52AVWAAKK129 pKa = 9.58HH130 pKa = 5.99RR131 pKa = 11.84SLGGNGKK138 pKa = 9.52RR139 pKa = 11.84CFDD142 pKa = 4.56HH143 pKa = 8.3LDD145 pKa = 3.54EE146 pKa = 4.72SAPWMGTCQHH156 pKa = 6.24GKK158 pKa = 9.01QFARR162 pKa = 11.84YY163 pKa = 7.65RR164 pKa = 11.84QPKK167 pKa = 10.3SMTGWACKK175 pKa = 9.91CSAGSTPITWRR186 pKa = 11.84RR187 pKa = 11.84NRR189 pKa = 3.5

Molecular weight: 20.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

19170

40

1329

193.6

20.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.14 ± 0.466

1.158 ± 0.152

6.463 ± 0.248

5.832 ± 0.348

2.608 ± 0.179

9.009 ± 0.412

2.165 ± 0.173

3.49 ± 0.15

3.031 ± 0.189

8.701 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.099

2.697 ± 0.23

5.477 ± 0.213

3.417 ± 0.153

7.355 ± 0.346

5.008 ± 0.163

5.681 ± 0.186

8.07 ± 0.248

2.144 ± 0.107

2.311 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski