Escherichia phage SSL-2009a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dhillonvirus; Escherichia virus SSL2009a

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2DRR6|T2DRR6_9CAUD Putative holin-like class I protein OS=Escherichia phage SSL-2009a OX=2681619 PE=4 SV=1
MM1 pKa = 7.32TPALMAGFSQKK12 pKa = 10.29FISVDD17 pKa = 3.0ACIADD22 pKa = 3.62YY23 pKa = 11.33EE24 pKa = 4.26MDD26 pKa = 5.54GIATVCDD33 pKa = 4.37GDD35 pKa = 3.97TKK37 pKa = 10.66TADD40 pKa = 3.69EE41 pKa = 4.54IEE43 pKa = 4.33EE44 pKa = 4.44TEE46 pKa = 4.01QQ47 pKa = 3.81

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T2DR30|T2DR30_9CAUD BIG2 domain-containing protein OS=Escherichia phage SSL-2009a OX=2681619 PE=4 SV=1
MM1 pKa = 7.46SATTRR6 pKa = 11.84DD7 pKa = 3.61MLQHH11 pKa = 5.81NRR13 pKa = 11.84FEE15 pKa = 4.13IGGRR19 pKa = 11.84VFRR22 pKa = 11.84NYY24 pKa = 9.44FVARR28 pKa = 11.84AWARR32 pKa = 11.84HH33 pKa = 4.64IGKK36 pKa = 9.03PEE38 pKa = 3.66GAIRR42 pKa = 11.84CYY44 pKa = 10.59TMM46 pKa = 4.87

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14246

29

1139

212.6

23.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.663 ± 0.519

1.186 ± 0.162

6.402 ± 0.282

6.037 ± 0.284

3.713 ± 0.184

7.764 ± 0.274

1.832 ± 0.18

5.398 ± 0.269

6.128 ± 0.371

7.378 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.184

4.219 ± 0.222

3.861 ± 0.214

3.854 ± 0.267

6.114 ± 0.245

4.668 ± 0.218

6.198 ± 0.253

7.272 ± 0.273

1.614 ± 0.148

2.885 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski