Pseudothermotoga thermarum DSM 5069

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Thermotogaceae; Pseudothermotoga; Pseudothermotoga thermarum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7YU86|F7YU86_9THEM Flagellar FliJ protein OS=Pseudothermotoga thermarum DSM 5069 OX=688269 GN=Theth_0077 PE=3 SV=1
MM1 pKa = 7.11QKK3 pKa = 9.73YY4 pKa = 9.02RR5 pKa = 11.84CIICGYY11 pKa = 10.35VYY13 pKa = 10.7DD14 pKa = 4.82PAQGDD19 pKa = 3.73PDD21 pKa = 4.34GDD23 pKa = 3.96IEE25 pKa = 4.81PGTPFEE31 pKa = 4.76EE32 pKa = 5.1LPEE35 pKa = 4.58DD36 pKa = 3.85WVCPVCGASKK46 pKa = 10.85EE47 pKa = 4.12DD48 pKa = 3.85FEE50 pKa = 4.71PVEE53 pKa = 4.27EE54 pKa = 4.22

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7YXX7|F7YXX7_9THEM CinA-like protein OS=Pseudothermotoga thermarum DSM 5069 OX=688269 GN=Theth_0691 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.4RR12 pKa = 11.84QRR14 pKa = 11.84THH16 pKa = 5.72GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TPSGRR28 pKa = 11.84KK29 pKa = 8.37VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.49GRR39 pKa = 11.84WRR41 pKa = 11.84LAVV44 pKa = 3.35

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1928

0

1928

615132

31

1647

319.1

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.785 ± 0.052

0.965 ± 0.019

4.728 ± 0.038

7.469 ± 0.061

5.16 ± 0.054

6.529 ± 0.053

1.488 ± 0.022

7.863 ± 0.047

7.97 ± 0.054

10.371 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.024

3.878 ± 0.032

3.796 ± 0.034

2.929 ± 0.031

4.44 ± 0.03

5.631 ± 0.043

4.671 ± 0.029

8.324 ± 0.044

0.997 ± 0.022

3.622 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski