Sinorhizobium phage phiM9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6THB0|A0A0F6THB0_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiM9 OX=1636182 GN=Sm_phiM9_232 PE=4 SV=1
MM1 pKa = 7.69IYY3 pKa = 10.2SKK5 pKa = 10.67KK6 pKa = 10.37AAIRR10 pKa = 11.84LIEE13 pKa = 4.7ANLEE17 pKa = 4.13MIEE20 pKa = 4.29NVPFDD25 pKa = 4.28LDD27 pKa = 4.06RR28 pKa = 11.84PHH30 pKa = 6.83TLAKK34 pKa = 10.09VLRR37 pKa = 11.84AYY39 pKa = 10.56GLIEE43 pKa = 4.14EE44 pKa = 4.78GAYY47 pKa = 10.0DD48 pKa = 3.61QYY50 pKa = 11.64LEE52 pKa = 4.26EE53 pKa = 4.71YY54 pKa = 9.23ILRR57 pKa = 11.84GMCEE61 pKa = 3.9DD62 pKa = 3.34SAVRR66 pKa = 11.84SEE68 pKa = 4.6AEE70 pKa = 4.17CIYY73 pKa = 10.3DD74 pKa = 3.22QLMRR78 pKa = 11.84YY79 pKa = 9.26GFMYY83 pKa = 10.39VVEE86 pKa = 4.46DD87 pKa = 4.01QEE89 pKa = 4.47GDD91 pKa = 3.16EE92 pKa = 4.56DD93 pKa = 3.94

Molecular weight: 10.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6THH9|A0A0F6THH9_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiM9 OX=1636182 GN=Sm_phiM9_103 PE=4 SV=1
MM1 pKa = 7.61NIGEE5 pKa = 4.49CVEE8 pKa = 4.01VRR10 pKa = 11.84DD11 pKa = 4.31VPIRR15 pKa = 11.84HH16 pKa = 5.51QFDD19 pKa = 3.23VNRR22 pKa = 11.84RR23 pKa = 11.84YY24 pKa = 10.67VYY26 pKa = 10.25GPRR29 pKa = 11.84ALRR32 pKa = 11.84YY33 pKa = 9.18RR34 pKa = 11.84DD35 pKa = 3.76RR36 pKa = 11.84PSDD39 pKa = 3.92AIGRR43 pKa = 11.84VWRR46 pKa = 11.84LSKK49 pKa = 9.42NTYY52 pKa = 9.48RR53 pKa = 11.84FEE55 pKa = 4.28IYY57 pKa = 9.93RR58 pKa = 11.84SSVRR62 pKa = 11.84YY63 pKa = 9.34KK64 pKa = 10.55FRR66 pKa = 11.84NASYY70 pKa = 11.29KK71 pKa = 10.29DD72 pKa = 3.47VCGKK76 pKa = 8.98ATTFSSALSEE86 pKa = 4.33LIRR89 pKa = 11.84RR90 pKa = 11.84YY91 pKa = 10.21RR92 pKa = 11.84GG93 pKa = 3.02

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

271

0

271

47955

15

1737

177.0

20.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.406 ± 0.165

1.009 ± 0.078

6.602 ± 0.143

7.538 ± 0.222

4.973 ± 0.127

6.116 ± 0.22

2.008 ± 0.11

6.429 ± 0.127

6.873 ± 0.217

7.874 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.097

4.807 ± 0.132

3.497 ± 0.1

3.057 ± 0.087

5.468 ± 0.143

6.708 ± 0.193

5.962 ± 0.206

7.102 ± 0.189

1.314 ± 0.07

3.664 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski