Lactobacillus nasuensis JCM 17158

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus nasuensis

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1JRH5|A0A0R1JRH5_9LACO R3H domain-containing protein OS=Lactobacillus nasuensis JCM 17158 OX=1291734 GN=FD02_GL001716 PE=4 SV=1
MM1 pKa = 6.42ATARR5 pKa = 11.84IVYY8 pKa = 10.71ASMTGNNEE16 pKa = 3.78EE17 pKa = 3.73IAGIVEE23 pKa = 4.26EE24 pKa = 4.2EE25 pKa = 4.19LEE27 pKa = 4.39KK28 pKa = 11.5YY29 pKa = 10.27GVTVTTTEE37 pKa = 4.02VSQAMAEE44 pKa = 4.17DD45 pKa = 4.34FQTVDD50 pKa = 2.68ICIVATYY57 pKa = 8.89TYY59 pKa = 10.73SDD61 pKa = 3.4VGDD64 pKa = 3.68GVLPDD69 pKa = 3.75EE70 pKa = 5.41AVDD73 pKa = 4.49FYY75 pKa = 11.8EE76 pKa = 5.85DD77 pKa = 3.96LQEE80 pKa = 5.34LDD82 pKa = 3.78LTGKK86 pKa = 10.65VYY88 pKa = 10.67GCCGSGDD95 pKa = 3.48KK96 pKa = 10.85FYY98 pKa = 11.28DD99 pKa = 3.89DD100 pKa = 4.58FATSVDD106 pKa = 4.04DD107 pKa = 4.06FDD109 pKa = 4.79HH110 pKa = 7.05AFAATGATKK119 pKa = 10.23GAPSVKK125 pKa = 9.09IDD127 pKa = 4.77LAPEE131 pKa = 4.77DD132 pKa = 4.78DD133 pKa = 6.05DD134 pKa = 4.59IAALEE139 pKa = 4.55AFTKK143 pKa = 10.71AIVEE147 pKa = 4.33KK148 pKa = 10.53QQSLL152 pKa = 3.59

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1JW37|A0A0R1JW37_9LACO DUF4097 domain-containing protein OS=Lactobacillus nasuensis JCM 17158 OX=1291734 GN=FD02_GL001550 PE=4 SV=1
MM1 pKa = 7.32VGPLTKK7 pKa = 10.38LGRR10 pKa = 11.84KK11 pKa = 8.33LRR13 pKa = 11.84KK14 pKa = 9.47APAKK18 pKa = 9.87HH19 pKa = 4.47SHH21 pKa = 6.11RR22 pKa = 11.84VLGIWVGKK30 pKa = 7.7SRR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 9.59EE35 pKa = 3.89MNRR38 pKa = 11.84QAANNRR44 pKa = 11.84NQPSGVLLSRR54 pKa = 11.84SGG56 pKa = 3.2

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2092

0

2092

663238

49

2404

317.0

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.824 ± 0.1

0.38 ± 0.013

5.626 ± 0.062

4.375 ± 0.049

3.825 ± 0.039

7.375 ± 0.056

2.339 ± 0.025

5.565 ± 0.054

4.408 ± 0.062

10.029 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.025

3.555 ± 0.04

4.428 ± 0.033

4.671 ± 0.04

4.647 ± 0.05

5.059 ± 0.083

7.343 ± 0.077

7.566 ± 0.047

1.222 ± 0.021

3.262 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski