Microbacterium lacticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y3UNQ1|A0A4Y3UNQ1_9MICO Uncharacterized protein OS=Microbacterium lacticum OX=33885 GN=FHX68_0982 PE=4 SV=1
MM1 pKa = 6.61NTLRR5 pKa = 11.84RR6 pKa = 11.84ALTVVAALAVASLTLSGCLYY26 pKa = 10.79AAIPPEE32 pKa = 4.47TSATPAPSRR41 pKa = 11.84EE42 pKa = 4.0PDD44 pKa = 3.59LDD46 pKa = 3.66GAPAGFEE53 pKa = 3.89EE54 pKa = 6.39FYY56 pKa = 11.11GQTLSWTPCSGSDD69 pKa = 3.72AGSYY73 pKa = 10.96DD74 pKa = 3.99CTDD77 pKa = 2.82VAAPLDD83 pKa = 3.76WSDD86 pKa = 3.68PASGTIEE93 pKa = 3.81LAVIRR98 pKa = 11.84RR99 pKa = 11.84AATSGDD105 pKa = 3.46ALGSLLTNPGGPGASGYY122 pKa = 11.21DD123 pKa = 3.53LVADD127 pKa = 3.77SASFAVSEE135 pKa = 4.5PVLDD139 pKa = 4.62AYY141 pKa = 10.66DD142 pKa = 3.77VIGFDD147 pKa = 3.45PRR149 pKa = 11.84GVGRR153 pKa = 11.84STAVACLDD161 pKa = 3.68AAGMDD166 pKa = 3.32HH167 pKa = 6.78MLYY170 pKa = 10.34DD171 pKa = 3.93IPADD175 pKa = 3.6PRR177 pKa = 11.84GSQGWTDD184 pKa = 3.76EE185 pKa = 3.93LTQRR189 pKa = 11.84NTEE192 pKa = 3.94FVEE195 pKa = 4.35ACEE198 pKa = 4.23ANSAGILPFVTTDD211 pKa = 2.84NAARR215 pKa = 11.84DD216 pKa = 3.92MDD218 pKa = 4.55LLRR221 pKa = 11.84AVLGDD226 pKa = 3.2KK227 pKa = 9.37TLNYY231 pKa = 10.2LGYY234 pKa = 10.53SYY236 pKa = 10.59GTFLGATYY244 pKa = 10.93AKK246 pKa = 10.27LFPEE250 pKa = 4.08RR251 pKa = 11.84VGRR254 pKa = 11.84LVLDD258 pKa = 4.15GAIDD262 pKa = 4.02PSASGLDD269 pKa = 3.35VGTTQAIGFEE279 pKa = 4.41SALRR283 pKa = 11.84AYY285 pKa = 9.48MADD288 pKa = 3.54CLDD291 pKa = 3.67GRR293 pKa = 11.84GCPFTGTVDD302 pKa = 3.33QAMADD307 pKa = 3.86LGTLLASVDD316 pKa = 4.04AQPLPADD323 pKa = 4.33DD324 pKa = 4.74GRR326 pKa = 11.84MLGADD331 pKa = 3.45TLMTGIIAALYY342 pKa = 10.83AEE344 pKa = 5.31DD345 pKa = 3.39SWPYY349 pKa = 8.98LTQALSQALQGDD361 pKa = 4.23PTTALLLADD370 pKa = 5.19FYY372 pKa = 11.65NGRR375 pKa = 11.84NSDD378 pKa = 2.92GTYY381 pKa = 10.34QDD383 pKa = 3.48NSTEE387 pKa = 3.7AFRR390 pKa = 11.84AYY392 pKa = 11.17NCMDD396 pKa = 3.63YY397 pKa = 11.03PLDD400 pKa = 3.9TSQADD405 pKa = 3.28QDD407 pKa = 3.83AANALIAQKK416 pKa = 10.8APTIAPYY423 pKa = 8.53WQGVDD428 pKa = 3.44VCEE431 pKa = 4.11VWPYY435 pKa = 10.35PPTGVRR441 pKa = 11.84EE442 pKa = 4.28RR443 pKa = 11.84IAADD447 pKa = 3.63GAAPIVVVGTTNDD460 pKa = 3.63PATPYY465 pKa = 10.25AWSVSLAEE473 pKa = 4.11QLSSGVLVTRR483 pKa = 11.84EE484 pKa = 4.46GEE486 pKa = 4.08GHH488 pKa = 5.7TGYY491 pKa = 11.19NKK493 pKa = 10.73GNTCVDD499 pKa = 3.71RR500 pKa = 11.84AVEE503 pKa = 4.54DD504 pKa = 3.87YY505 pKa = 11.05LVDD508 pKa = 3.52GTVPQDD514 pKa = 3.54GLSCC518 pKa = 4.38

Molecular weight: 54.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543KRX0|A0A543KRX0_9MICO Phosphoglycolate phosphatase OS=Microbacterium lacticum OX=33885 GN=FHX68_1824 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2765

0

2765

875069

31

2270

316.5

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.175 ± 0.076

0.501 ± 0.012

6.397 ± 0.045

5.554 ± 0.052

2.954 ± 0.027

8.9 ± 0.048

2.067 ± 0.024

4.407 ± 0.033

2.025 ± 0.033

9.847 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.714 ± 0.018

1.824 ± 0.021

5.399 ± 0.033

2.791 ± 0.028

7.713 ± 0.059

5.294 ± 0.034

6.119 ± 0.044

8.839 ± 0.046

1.487 ± 0.02

1.994 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski